Method for detecting 7 sweeteners in fruit juice by means of super-efficient liquid chromatography-tandem mass spectrometry

A technology of ultra-high performance liquid chromatography and tandem mass spectrometry, which is applied in the field of detection of sweeteners in fruit juices, can solve the problem of single detection parameters, and achieve the effects of simple operation, good extraction effect and accurate results

Inactive Publication Date: 2017-07-28
FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been some reports using this method to detect sweeteners in food, but these reports have a single detection parameter, and most of them are aimed at wine products, dairy products and condiments, etc., cyclamate (SC) and sodium saccharin ( The simultaneous detection of seven sweeteners including SA), acesulfame-K (AK), sucralose (SL), aspartame (ASP), neotame (NTM) and stevioside (STV) has not been reported yet.

Method used

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  • Method for detecting 7 sweeteners in fruit juice by means of super-efficient liquid chromatography-tandem mass spectrometry
  • Method for detecting 7 sweeteners in fruit juice by means of super-efficient liquid chromatography-tandem mass spectrometry
  • Method for detecting 7 sweeteners in fruit juice by means of super-efficient liquid chromatography-tandem mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049]Utilize ultra-high performance liquid chromatography-tandem mass spectrometry to detect the method for 7 kinds of sweeteners in fruit juice simultaneously, comprise the steps:

[0050] 1) Preparation of test samples

[0051] Accurately draw 5mL of apple juice sample into a 50mL centrifuge tube, use methanol / water (v:v=1:1) to extract the solvent and dilute to 10mL, shake well, centrifuge at 9000r / min for 3min, and draw the supernatant through a 0.22μm polymer Ethersulfone filter membrane, prepared test sample;

[0052] 2) Detection by ultra-high performance liquid chromatography-tandem mass spectrometry

[0053] The chromatographic conditions are as follows:

[0054] Chromatographic column: HSS T3 column (100mm×2.1mm, 1.8μm); column temperature 40°C, mobile phase: A is methanol, B is ultrapure water; flow rate: 0.4mL / min; injection volume 5μL. Electrospray (ESI) ion source; negative ion mode; MRM (Multiple Reaction Monitoring); ion source temperature 150°C; desolvatio...

experiment example

[0074] 1 Materials and methods

[0075] 1.1 Instruments and reagents

[0076] UPLC-MS / MS XEVO TQ ultra-high performance liquid chromatography-tandem mass spectrometer: Waters Company of the United States; vertical large-capacity high-speed centrifuge: HITACHI Company of Japan; Milli-Q pure water / ultrapure water integrated intelligent system: Millipore Company of the United States .

[0077] Methanol, ammonium acetate (HPLC grade): American Thermo Fisher Scientific Company; acetic acid (HPLC grade): American J.T.Baker Company; test water: prepared by Milli-Q ultrapure water system.

[0078] Water for seven standard products of cyclamate (SC), sodium saccharin (SA), acesulfame potassium (AK), sucralose (SL), aspartame (ASP), neotame (NTM), and stevioside (STV) Prepare a stock solution of 1000 mg / kg, prepare a mixed standard working solution of appropriate concentration according to needs, and store it in a -20°C refrigerator.

[0079] 1.2 Test conditions

[0080] Table 1 Mas...

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Abstract

The invention provides a method for detecting 7 sweeteners in fruit juice by means of super-efficient liquid chromatography-tandem mass spectrometry and belongs to the field of food detection and analysis. The method comprises: preparing a sample for test and detecting by means of super-efficient liquid chromatography-tandem mass spectrometry; the 7 sweeteners include sodium cyclamate, saccharin sodium, acesulfame potassium, sucralose, aspartame, neotame and stevioside, wherein an extracting solvent for preparing a sample for test is methanol / water (v:v=1 / 1), acetonitrile / water (v:v=1 / 1) or purified water. The super-efficient liquid chromatography-tandem mass spectrometry method for detecting 7 sweeteners in fruit juice at the same time is established by optimizing mobile phase, column temperature and extracting solvent conditions; the method is quick, simple, efficient, economical and accurate; methodology verification for precision, accuracy and the like shows that the method is applicable to the detection of 7 sweeteners in fruit juice.

Description

technical field [0001] The invention relates to a method for detecting sweeteners in fruit juice, and belongs to the technical field of food detection and analysis. Background technique [0002] In recent years, the fruit juice beverage industry has developed rapidly in my country as an important way to solve the difficulty of selling fruit and increase the income of fruit farmers. In order to increase its economic benefits, some manufacturers add a variety of sweeteners to the fruit juice to improve the taste, but the amount of addition directly affects the health of consumers, and the safety problems brought about are increasingly prominent. Food testing and monitoring Sweeteners in food have become one of the important tasks of the safety monitoring department. Therefore, it is necessary to develop a rapid, accurate and sensitive analytical method for the determination of sweeteners in fruit juices to ensure their quality and safety. [0003] At present, the detection m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88G01N2030/884
Inventor 闫震聂继云李志霞李静沈友明李海飞毋永龙匡立学程杨关棣锴李明强徐国锋
Owner FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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