Preparation method of β-configuration decitabine precursor
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A decitabine and configuration technology, applied in the field of preparation of decitabine precursors, can solve problems such as unfavorable enrichment of β-type products, and achieve the effects of simple post-processing, improved selectivity and good repeatability
Inactive Publication Date: 2018-04-24
ZHENGZHOU TECHN COLLEGE +1
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[0006] In short, the prior art of directly using methoxy for direct coupling all teaches that it is not conducive to the enrichment of β-type products. Most of the literature is to convert the C1-position group, usually to a halogen or acyl group, and then perform coupling
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Embodiment 1
[0018] 60 mmol of 2,4-bis-(trimethylsilyl)-5-azacytosine with 50 mmol of 1-(2-butenyl)oxy-2-deoxy-3,5-di-O-acetyl -D-ribofuranose was dissolved in 500 mL of acetonitrile, the temperature was controlled to 0 °C, 10 mmol of trifluoromethanesulfonic acid was added, and 0 °C was continued, and the reaction was stirred for 2 hours, and the reaction was completed by HPLC monitoring. Quickly quench the reaction and wash the reaction mixture with 500 mL of saturated aqueous sodium bicarbonate solution, separate the organic layer, evaporate it to dryness under vacuum, and then recrystallize with toluene to obtain 14.75 g of solid, which is identified as 1-(2- Deoxy-3,5-di-O-acetyl-D-ribose)-4-amino-1,3,5-s-triazin-2-one, its purity detected by HPLC is 90%, and its β configuration is the same as α The content ratio of the configuration was 2.5.
[0021] Use 1-allyloxy-2-deoxy-3,5-di-O-acetyl-D-ribofuranose to directly carry out the coupling reaction. For each condition, refer to the example cytosine raw material 1,2,4-di-( The amount of trimethylsilyl)-5-azacytosine is different, and the results are summarized as follows:
[0022]
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Abstract
The invention provides a preparation method of a beta-configuration decitabine precursor. The preparation method comprises the following step: under the action of a Lewis acid catalyst, directly performing a coupling reaction on acylated sugar adopting the structure of 1-alkenylmethoxy-2-deoxy-3, 5-di-O-acetyl-D-ribofuranose and a silicon ether compound of 5-azacytosine to obtain beta-configuration 1-(2-deoxy-3,5-di-O-acetyl-D-ribose)-4-amino-1,3,5-s-triazine-2-one. The beta-configuration decitabine precursor is high in selectivity, good in repeatability and very high in molar yield and purity.
Description
technical field [0001] The present invention relates to a preparation method of decitabine precursor. Background technique [0002] The preparation process of decitabine is disclosed in many documents, and is roughly divided into three categories: isocyanate method, azacitidine deoxygenation method, and glycosidation method. The characteristic of glycosidation method is that the pyrimidine part is coupled with the ribose part, and the coupling reaction is obtained. The product of hydroxy-protected decitabine (referred to as decitabine precursor in this application) is finally deprotected (usually hydrolyzed) to obtain decitabine. As we all know, decitabine is a beta isomer, but it is usually obtained as a mixture of alpha and beta during preparation. Obviously, the content ratio of beta decitabine in the product and its purification complexity Both were directly affected by the proportion of beta form in the decitabine precursor before hydrolysis. [0003] Before coupling,...
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