Raman coding microspheres and preparation method thereof

Abstract

The invention discloses Raman coding microspheres and a preparation method thereof, wherein the Raman coding microspheres are obtained by coating multi-layer metal nanoparticles on the surfaces of inner core nanoparticles through a layer-by-layer self-assembly method and marking with a Raman active substance. The preparation method and raw materials of the Raman coding microspheres are simple, and the strength of Raman signals of a marker can be regulated and controlled through the number of assembly layers of the metal nanoparticles; compared with the existing Raman making technology, the Raman coding microspheres according to the invention are strong in the Raman signals, uniform in aggregation state of the particles and good in repeatability of the Raman signals.

Description

1. Technical field

[0001] The invention relates to the technical fields of nanometer material science and laser Raman detection, in particular to a Raman coded microsphere and a preparation method thereof. 2. Background technology

[0002] Surface-enhanced Raman scattering (SERS) is a fingerprint spectroscopy technique with high sensitivity, which can identify and detect species adsorbed on the surface of nanostructures at the molecular level, and its enhancement ability can reach 10 14 -10 15 . SERS technology is not disturbed by the water environment, nor will photobleaching and self-quenching occur. SERS active substrates with different shapes and sizes have been widely used in the detection and imaging of biological species. The simplest way is to use the enhancement of the vibration signal of the molecule to be tested for label-free detection, but in some cases, it is also necessary to introduce a SERS active label or reporter group into the detection system to produc...

Images