Tremella heteropolysaccharide and its extract, preparation method and application

A technology of tremella heteropolysaccharides and extracts, which is applied in the direction of pharmaceutical formulas, cosmetic preparations, cosmetic preparations, etc., can solve the problems of dark color and low purity of tremella heteropolysaccharides, so as to increase water storage capacity and promote good health. Fibroblast proliferation effect, excellent skin permeability effect

Active Publication Date: 2019-11-08
SHANGHAI HUIWEN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a tremella heteropolysaccharide and its extract and preparation method in order to overcome the problems of low purity and dark color in the method of obtaining tremella heteropolysaccharide by alkali hydrolysis of glycosidic bonds in the prior art and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Take white fungus fruit body raw materials, soak them in pure water and wash them; after washing the raw materials, add 6% sodium hydroxide aqueous solution (mL) with a concentration of 20 times the weight of raw materials (g), and extract by alkaline hydrolysis at 90°C to 98°C for 4 hours After the alkaline hydrolysis, adjust the pH value of the feed solution to 6.0 to 7.0 with 6% dilute hydrochloric acid; carry out conventional decompression fine filtration, and collect the filtrate; add 8ml of 1% acidic flocculant solution in every 100ml of filtrate. Stir under the conditions to fully flocculate to form a precipitated complex; filter to collect the precipitated complex; and discard the filtrate after harmless treatment. Disperse the above precipitated complex in a pure aqueous solution (mL) of 20 times the volume of the raw material weight (g), and use 5% sodium hydroxide solution to adjust the pH value of the feed solution to alkaline 9.0-10.0. Thermal dissociation ...

Embodiment 2

[0040] Take white fungus fruit body raw materials, soak them in pure water and wash them; after washing the raw materials, add 5% sodium hydroxide aqueous solution (mL) with a concentration of 5% sodium hydroxide solution (mL) 25 times the weight of raw materials (g), and extract by alkaline hydrolysis at 90°C to 98°C for 3 hours , after alkaline hydrolysis, adjust the pH value of the feed solution to 6.0-7.0 with dilute hydrochloric acid with a mass concentration of 6%; carry out conventional fine filtration and collect the filtrate; add 5ml of 2% acidic flocculant solution in every 100ml of filtrate, under normal temperature conditions Stir under low pressure to fully flocculate to form a precipitated complex; filter to collect the precipitated complex; and discard the filtrate after harmless treatment. Disperse the above precipitated complex in a pure aqueous solution (mL) of 15 times the volume of the raw material weight (g), and use 5% sodium hydroxide solution to adjust t...

Embodiment 3

[0042] Take white fungus fruit body raw materials, soak them in pure water and wash them; after cleaning the raw materials, add 8% sodium hydroxide aqueous solution (mL) with a concentration of 15 times the volume of the raw materials weight (g), and extract them by alkaline hydrolysis at 90°C to 98°C 6h; After the alkaline hydrolysis, use 6% dilute hydrochloric acid to adjust the pH value of the feed solution to 6.0-7.0; carry out conventional fine filtration and collect the filtrate; add 10 mL of 0.5% acidic flocculant solution to each 100 mL of filtrate, Stir under low pressure to fully flocculate to form a precipitated complex; filter to collect the precipitated complex; and discard the filtrate after harmless treatment. Disperse the above-mentioned precipitated complex in a pure aqueous solution (mL) of 10 times the volume of the raw material weight (g), and adjust the pH value of the feed solution to alkaline 8.0-9.0 with 5% sodium hydroxide solution. Thermal dissociatio...

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Abstract

The invention discloses tremella fuciformis sporocarp extract as well as extract, a preparation method and application thereof. The preparation method of the tremella fuciformis sporocarp extract, disclosed by the invention, comprises the following steps: mixing an acidic flocculating agent with a tremella alkalie extracting solution which is regulated to be weak acidic or neutral by utilizing acid; flocculating and carrying out solid-liquid separation to obtain a sediment compound, wherein the acidic flocculating agent is an acidic water solution of the flocculating agent and the flocculating agent is chitosan. By utilizing the preparation method disclosed by the invention, the tremella fuciformis sporocarp extract with high purity and different molecular weight ranges can be prepared, the yield can reach 20 percent or more and the purity can reach 93 percent or more; the tremella fuciformis sporocarp extract with the molecular weight of 0.1 to 0.2 million Daltons, which is prepared by the preparation method, has a good fibroblast proliferation promoting effect and a capability of prompting fibroblasts to secrete hyaluronic acid; meanwhile, the tremella fuciformis sporocarp extract also has excellent percutaneous permeability.

Description

technical field [0001] The invention relates to tremella heteropolysaccharide and its extract, preparation method and application. Background technique [0002] Tremella heteropolysaccharide, as a functional polysaccharide, can be used in medicine, health products, food and cosmetics, and has various functions. Patent CN100402555C discloses a tremella heteropolysaccharide with a molecular weight of 850,000 to 1,600,000 Daltons extracted under high temperature conditions in aqueous solution, and discloses that it has moisturizing and lubricating skin and mucous membranes, enhanced lubricating feeling, anti-oxidative aging, anti-allergic, anti-inflammatory Thick stability and other effects. Patent CN102161710A discloses a method for preparing low-molecular-weight tremella heteropolysaccharides by acid hydrolysis, and discloses the use of tremella heteropolysaccharides with a molecular weight of 500-10,000 Daltons in the preparation of drugs for treating chronic atrophic gastr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K8/73A61K8/9728A61Q19/00A61Q19/08
CPCA61K8/73A61Q19/00A61Q19/08C08B37/0003C08B37/006
Inventor 骆峰周自华杨海延
Owner SHANGHAI HUIWEN BIO TECH
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