Christina loosestrife herb polysaccharide with immunity-strengthening effect and preparation method and application thereof

A technology of polysaccharide and immune function of S. chinensis is applied in the field of biomedicine, which can solve the problems of obvious adverse reactions and inaccurate effects, and achieve the effects of small adverse reactions, simple operation and simple preparation method.

Inactive Publication Date: 2017-08-04
成都众宜坊农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: to provide a desmodium polysaccharide that has the function of increasing immuni

Method used

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  • Christina loosestrife herb polysaccharide with immunity-strengthening effect and preparation method and application thereof
  • Christina loosestrife herb polysaccharide with immunity-strengthening effect and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of desmodium polysaccharide.

[0029] The preparation method of desmodium polysaccharide comprises the following steps:

[0030] A: Degreasing: Take Desmodium, add 5 times the amount of 95% ethanol, heat and reflux for extraction for 2 hours, filter, evaporate the ethanol from Desmodium, and dry at 60°C to obtain Degreased Desmodium;

[0031] B: water extraction: add water to the degreased Desmodium and heat extraction, the condition of adding water and heating to extract is 90°C and add water to extract twice, each extraction is 1.5 hours, and the amount of water added each time is 6 times the quality of the Desmodium, The extract is filtered, and the filtrate is concentrated into a concentrated solution with a density of 1.05 (60°C);

[0032] C: Alcohol precipitation: Add 95% ethanol to the concentrated solution to make the alcohol content 75%, refrigerate at 4°C, let it stand for 24 hours, centrifuge, and take the precipitate;

[0033] D: After dissolvi...

Embodiment 2

[0037] Preparation of desmodium polysaccharide.

[0038] The preparation method of desmodium polysaccharide comprises the following steps:

[0039] A: Degreasing: Take Desmodium, add 5 times the amount of 95% ethanol, heat and reflux for extraction for 2 hours, filter, evaporate the ethanol from Desmodium, and dry at 50°C to obtain Degreased Desmodium;

[0040] B: water extraction: add water to the degreased Desmodium and heat extraction, the condition of adding water and heating to extract is 90°C and add water to extract twice, each extraction is 1.5 hours, and the amount of water added each time is 6 times the quality of the Desmodium, The extract is filtered, and the filtrate is concentrated into a concentrated solution with a density of 1.05 (45°C);

[0041] C: Alcohol precipitation: Add 80% ethanol to the concentrated solution to make the alcohol content 60%, refrigerate at 0°C, let it stand for 48 hours, centrifuge, and take the precipitate;

[0042] D: After dissolvi...

Embodiment 3

[0046] Preparation of desmodium polysaccharide.

[0047] The preparation method of desmodium polysaccharide comprises the following steps:

[0048] A: Degreasing: Take Desmodium, add 5 times the amount of 95% ethanol, heat and reflux for extraction for 2 hours, filter, evaporate the ethanol from Desmodium, and dry at 50°C to obtain Degreased Desmodium;

[0049] B: water extraction: add water to the degreased Desmodium and heat extraction, the condition of adding water and heating to extract is 90°C and add water to extract twice, each extraction is 1.5 hours, and the amount of water added each time is 6 times the quality of the Desmodium, The extract is filtered, and the filtrate is concentrated into a concentrated solution with a density of 1.05 (50°C);

[0050] C: Alcohol precipitation: Add 90% ethanol to the concentrated solution to make the alcohol content 68%, refrigerate at 6°C, let it stand for 24 hours, centrifuge, and take the precipitate;

[0051] D: After dissolvi...

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Abstract

The invention discloses a christina loosestrife herb polysaccharide with an immunity-strengthening effect and solves the problems that a drug for strengthening the human immunity is obvious in adverse reaction and inaccurate in effect in the prior art. The christina loosestrife herb polysaccharide is prepared through degreasing christina loosestrife herb, carrying out water extraction and alcohol precipitation, intercepting a component of which the molecular weight is greater than 10,000Dalton through dialysis and separating through macroporous weak-base anion-exchange resin. The preparation method comprises the following steps of degreasing, water extraction, alcohol precipitation, dialysis, macroporous weakly basic anion exchange resin column chromatography and drying. The christina loosestrife herb polysaccharide is applied to preparation of foods and health foods or drugs with the immunity-strengthening effect. The christina loosestrife herb polysaccharide is accurate in immunity-strengthening effect, small in adverse reaction, simple in preparation method, simple in operation and suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a desmodium polysaccharide with the function of increasing immunity, its preparation method and application. Background technique [0002] Desmodium is the dry whole plant of Lysimachia christinae Hance of Primulaceae. Harvest in summer and autumn, remove impurities and dry in the sun. Desmodium is sweet and salty in taste, slightly cold in nature. Return liver, gallbladder, kidney, urinary bladder channel. It has the effects of promoting dampness and reducing jaundice, promoting diuresis and removing stranguria, detoxifying and reducing swelling. It is used for damp-heat jaundice, gallbladder distension and hypochondriac pain, stone stranguria, hot stranguria, astringent pain in urination, carbuncle and furuncle, and snake bites. [0003] Modern studies have shown that the whole herb of Desmodium contains flavonoids: quercetin, isoquercitrin, quercetin-3-O-gluc...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P37/04A23L33/125
CPCC08B37/0003A23V2002/00A61K31/715C08B37/006A23V2200/324A23V2250/51
Inventor 孔利文辜晓平
Owner 成都众宜坊农业开发有限公司
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