Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of laminarin

The technology of kelp polysaccharide and kelp is applied in the field of kelp polysaccharide preparation, which can solve the problem that the kelp polysaccharide cannot be fully utilized by the human body, and achieve the effect of increasing the content.

Active Publication Date: 2021-01-29
泰安合生世纪生物科技有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above deficiencies, the present invention provides a method for preparing laminarin polysaccharides. Using the method, the nutritional components in laminarin polysaccharides obtained in laminarium are converted into edible fungus polysaccharides by edible fungi, which are easily absorbed by the human body, and the potency of laminarin polysaccharides is improved. The utilization rate overcomes the defect that laminarin cannot be fully utilized by the human body

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for preparing laminarin, comprising the steps of:

[0037] (1) Preparation of liquid medium, wash potatoes, peel, slice, weigh 25g, weigh 5g bran, add appropriate amount of water, boil for 20-30 minutes, filter, add 1g to the filtrate Sucrose, 0.015g MgSO 4 and 0.075g of KH 2 PO 4 , add water to the filtrate to make up to 1L, then put the culture medium into suction filter bottles, and carry out high-pressure sterilization in a pressure cooker with a sterilization pressure of 0.12MPa, a temperature of 121.3°C, and a sterilization time of 30 minutes.

[0038] (2) Inoculation Inoculate the strains of Ganoderma lucidum, Cordyceps militaris, Grifola frondosa and Poria cocos in the liquid medium prepared in the previous step. 25-28°C.

[0039] (3) Preparation of kelp culture medium

[0040] First, weigh the ingredients of the medium according to the formula of the medium, kelp granules 93Kg, kelp granules use desalted dry granules, sucrose 1Kg, CaCO 3 1Kg, KH...

Embodiment 2

[0047] (1) Preparation of liquid medium: wash potatoes, peel them, slice them, weigh 30g, weigh 10g of bran, add appropriate amount of water, boil for 20-30 minutes, filter, add 3g to the filtrate Sucrose, 0.015g MgSO 4 and 0.075g of KH 2 PO 4 , add water to the filtrate to make up to 1L, then put the culture medium into suction filter bottles, and carry out high-pressure sterilization in a pressure cooker with a sterilization pressure of 0.12MPa, a temperature of 121.3°C, and a sterilization time of 30 minutes.

[0048] (2) Inoculation Inoculate Ganoderma lucidum, Cordyceps militaris, Grifola frondosa, and Poria cocos strains in the liquid medium prepared in the previous step, and carry out liquid culture for 5-7 days at a temperature of 25-28°C.

[0049] (3) Preparation of kelp culture medium

[0050] First, according to the medium formula, weigh the ingredients of the medium, kelp granules 96Kg, kelp granules use desalted dry granules, sucrose 3Kg, CaCO 3 3Kg, KH 2 PO...

Embodiment 3

[0057] (1) Preparation of liquid medium, wash potatoes, peel, slice, weigh 28g, weigh 8g bran, add appropriate amount of water, boil for 20-30 minutes, filter, add 2g to the filtrate Sucrose, 0.015g MgSO 4 and 0.075g of KH 2 PO 4 , add water to the filtrate to make up to 1L, then put the medium into suction filter bottles, and carry out high-pressure sterilization in a pressure cooker with a sterilization pressure of 0.12MPa, a temperature of 121.3°C, and a sterilization time of 30 minutes.

[0058] (2) Inoculation Inoculate the strains of Ganoderma lucidum, Cordyceps militaris, Grifola frondosa and Poria cocos in the liquid medium prepared in the previous step, and carry out liquid culture for 5-7 days at a temperature of 25-28°C.

[0059] (3) Preparation of kelp culture medium

[0060] First, according to the medium formula, weigh the ingredients of the medium, kelp granules 95Kg, kelp granules use desalted dry granules, sucrose 2Kg, CaCO 3 2Kg, KH 2 PO 4 0.05Kg, MgSO...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of kelp polysaccharide, which comprises the following steps: (1) preparation of liquid medium (2) inoculation (3) preparation of kelp medium (4) inoculation (5) cultivation (6) removal (7) Enzymatic hydrolysis and extraction (8) drying, puffing, and pulverizing the inactivated material to finally obtain laminarin. The laminarin obtained by this method is easily absorbed by the human body, and the laminarin obtained by this method is not easily absorbed by the human body. absorb. Through the method, the content of laminarin can be increased by nearly 4 times.

Description

technical field [0001] The invention relates to the field of preparation of laminarin, in particular to a preparation method of laminarin. Background technique [0002] Kelp polysaccharide refers to the polysaccharide components purified from kelp, including kelp oligosaccharides, fucoidan, and kelp starch. [0003] There are also many scholars who have studied and discussed the preparation methods of laminarin polysaccharides. The traditional methods are mainly acid extraction, alkali extraction, enzyme extraction and so on. There are certain defects in these preparation methods. Acid extraction can cause polysaccharide hydrolysis, and alkali extraction can cause polysaccharide biological activity to decrease. The enzyme extraction method is time-consuming, and industrial production requires a large amount of enzyme and high cost. The method of hot water extraction and alcohol precipitation is simple, but time-consuming and energy-consuming. The methods for removing pro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04
CPCC12P19/04
Inventor 周刚毅张继春
Owner 泰安合生世纪生物科技有限公司