Method for separating and refining 1, 2, 4-butantriol from microbial fermentation liquid

A refining method, butanetriol technology, applied in the fields of bioengineering and chemical engineering, can solve the problems of low efficiency, slow speed, complex components of fermentation products, etc., and achieve the effect of high separation efficiency and simple process

Inactive Publication Date: 2017-08-08
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some studies have used resins with boric acid groups as adsorbents for the adsorption of polyols, but the problem is that desorption is required after adsorption, and the desorbed eluent needs to be further separated after desorption, coupled with resin adsorption and Elution itself is slow and therefore not efficient
[0007] In addition, due to the complex composition of the fermentation product, the final high-purity product still needs to be achieved by means of distillation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating and refining 1, 2, 4-butantriol from microbial fermentation liquid
  • Method for separating and refining 1, 2, 4-butantriol from microbial fermentation liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The BT fermented liquid in which the BT concentration is about 3-6 g / L is heat-sterilized and plate-and-frame filtered to remove bacteria and flocculated solid protein in the fermented liquid.

[0025] Add the ethyl acetate solution of n-tetradecylboronic acid to the fermented broth that removes the thalline after filtration, the total volume ratio of ethyl acetate and aqueous solution is about 1:1, make described n-tetradecylboronic acid and fermented broth The molar ratio of BT is controlled at about 1:1, forming two phases with aqueous solution. Among them, under the condition of 60°C, add 0.46g of n-tetradecylboronic acid complexing agent into 10ml of ethyl acetate, stir and dissolve, add 10ml of 0.1mol / L sodium hydroxide solution, and adjust the pH value to about 13 , then add 10ml of BT fermentation broth, stir slowly moderately, and use the complexation between the boric acid reagent in the organic phase and the BT in the fermentation broth to perform selective c...

Embodiment 2

[0028] The concentration of BT in the BT fermentation liquid is about 3-6g / L, and the fermentation liquid is sterilized by heat and filtered by plate and frame to remove the bacteria and flocculated solid protein in the fermentation liquid.

[0029] Add ethyl acetate solution of phenylboronic acid to the fermented broth after filtering to remove the bacteria, the total volume ratio of ethyl acetate to aqueous solution is about 1:1, so that the molar ratio of BT in the phenylboronic acid to the fermented broth is controlled At about 1:1, form two phases with the aqueous solution, moderately and slowly stir, use the complexation between the boric acid reagent in the organic phase and the BT in the fermentation broth to perform selective complexation extraction, after stirring for 30min, to the fermentation broth The concentration of BT in the medium is less than 0.5g / L, separate the aqueous phase and the organic solvent phase, and repeat this 3 times, each time using the same amo...

Embodiment 3

[0032] The BT product was obtained in the same manner as in Example 1 except that 2-phenylethylboronic acid was used as the organic boric acid.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a microbial fermentation liquid separation technique, in particular to a method for separating and refining 1, 2, 4-butantriol from a microbial fermentation liquid. The method includes use of a specific organic boric acid selected from C10-C18 alkyl boronic acid or C6-C18 aryl boric acid for extraction of a fermentation liquid containing the 1, 2, 4-butantriol and separation by molecular distillation so as to efficiently obtain the 1, 2, 4-butantriol. The method for separating and refining combines the complexation extraction and the molecular distillation to separate and purify a BT produced by a fermentation process, the process is simple, the separation efficiency is high, and the method is favorable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical fields of bioengineering and chemical engineering, and relates to a separation technology of microbial fermentation liquid, in particular to a method for separating and refining 1,2,4-butanetriol from microbial fermentation liquid. Background technique [0002] 1,2,4-Butanetriol (hereinafter referred to as BT) is an important intermediate, which is widely used in the fields of medicine, tobacco, cosmetics, papermaking, agriculture and polymer materials. No natural BT biosynthesis pathway has been found in nature. At present, the industrial production of butanetriol mainly relies on chemical synthesis, which has many by-products, low yield, and is harmful to the environment. The clean and efficient biosynthesis pathway of butanetriol has gradually attracted research. attention. [0003] At present, the concentration of BT produced by biological fermentation is relatively low, generally at the level of a few grams ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07C29/88C07C29/80C07C29/74C07C31/22
CPCC07C29/74C07C29/80C07C29/88C07C31/22
Inventor 王建勋侯同刚咸漠董晴晴王乔
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap