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Isomalto-oligosaccharide refining production process

A technology of isomaltooligosaccharide and production process, which is applied in the direction of oligosaccharides, sugar derivatives, sugar derivatives, etc., and can solve problems such as difficult separation

Inactive Publication Date: 2017-08-11
安得膜分离技术工程(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the increasing market demand for isomaltooligosaccharide, in order to increase the added value of the product, it is sometimes necessary to separate polysaccharides with different carbon numbers in the crude isomaltooligosaccharide, but it is difficult to separate the crude isomaltooligosaccharide by chromatographic separation technology Polysaccharides with different carbon numbers

Method used

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  • Isomalto-oligosaccharide refining production process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The refined production process of isomalto-oligosaccharides includes the following steps:

[0040] a. Add water to the raw starch to adjust the slurry concentration, adjust the pH and add high-temperature resistant α-amylase to make the starch slurry. After liquefaction, a liquefied liquid is obtained; after the liquefied liquid is cooled, the pH is adjusted to 6.0-6.5, and then malt is added Trisaccharide-producing enzyme and pullulanase. After saccharification is kept for 36-56 hours, the enzyme is inactivated at 80-90°C to prepare a saccharification solution; α-glucosidyltransferase is added to the saccharification solution at 55-60°C Reaction and inactivation of enzymes to obtain crude isomaltose oligosaccharide solution;

[0041] b. The crude oligomeric isomaltose liquid is filtered through a plate and frame to remove particulate impurities, colloids and suspended solids in the water, and then pass through an ultrafiltration membrane to obtain a permeate and a retentate...

Embodiment 2

[0054] The refined production process of isomalto-oligosaccharides includes the following steps:

[0055] a. Add water to the raw starch to adjust the slurry concentration, adjust the pH and add high-temperature resistant α-amylase to make the starch slurry. After liquefaction, a liquefied liquid is obtained; after the liquefied liquid is cooled, the pH is adjusted to 6.0-6.5, and then malt is added Trisaccharide-producing enzyme and pullulanase. After saccharification is kept for 36-56 hours, the enzyme is inactivated at 80-90°C to prepare a saccharification solution; α-glucosidyltransferase is added to the saccharification solution at 55-60°C Reaction and inactivation of enzymes to obtain crude isomaltose oligosaccharide solution;

[0056] b. The crude oligomeric isomaltose liquid is filtered through a plate and frame to remove particulate impurities, colloids and suspended solids in the water, and then pass through an ultrafiltration membrane to obtain a permeate and a retentate...

Embodiment 3

[0069] The refined production process of isomalt oligosaccharide is characterized by comprising the following steps:

[0070] a. Add water to the raw starch to adjust the slurry concentration, adjust the pH and add high-temperature resistant α-amylase to make the starch slurry. After liquefaction, a liquefied liquid is obtained; after the liquefied liquid is cooled, adjust the pH to 6.0-6.5, and then add malt Trisaccharide-generating enzyme and pullulanase. After saccharification is kept for 36-56 hours, the enzyme is inactivated at 80-90°C to prepare a saccharification solution; α-glucosidyltransferase is added to the saccharification solution at 55-60°C Reaction and inactivation of enzymes to obtain crude isomaltose oligosaccharides;

[0071] b. The crude oligomeric isomaltose liquid is filtered through a plate and frame to remove particulate impurities, colloids and suspended solids in the water, and then pass through an ultrafiltration membrane to obtain a permeate and a retent...

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Abstract

The invention belongs to the field of isomalto-oligosaccharide and particularly relates to an isomalto-oligosaccharide refining production process. The isomalto-oligosaccharide refining production process includes steps: firstly, sequentially subjecting starch to liquefying, saccharifying and glucoside conversion reaction to obtain isomalto-oligosaccharide crude liquid; secondly, sequentially subjecting the isomalto-oligosaccharide crude liquid to microfiltration, ultrafiltration, primary nanofiltration, secondary nanofiltration, tertiary nanofiltration and reverse osmosis. According to the refining production process, saccharides, with different carbon numbers, in the isomalto-oligosaccharide crude liquid are separated by means of membrane separation to obtain corresponding saccharide concentrated liquid. The isomalto-oligosaccharide refining production process is great in separation effect and low in energy consumption, and the saccharide concentrated liquid obtained by separation can be used for preparation of saccharide products with corresponding carbon numbers for selling, so that additional values of products are greatly increased.

Description

Technical field [0001] The invention belongs to the field of isomalt oligosaccharides, and particularly relates to a refined production process of isomalt oligosaccharides. Background technique [0002] Isomaltooligosaccharides (IMO) are also known as isomalto-oligosaccharides, isomalto-oligosaccharides, and branched oligosaccharides. It is a kind of starch sugar, and its main component is isomaltose, panose, isomaltose, and oligosaccharides above tetrasaccharide bound by α-1,6 glycosidic bonds between glucose molecules. Because of its stable properties, isomalt oligosaccharide is safe and non-toxic as a food additive, is not digested by enzymes in the gastrointestinal tract, and has no residue in the human body. It is widely used in food, dairy products, and health products. It can improve the intestinal microflora, promote intestinal development, regulate protein and lipid metabolism, promote mineral absorption, enhance immunity, have the effect of antibiotics, can delay aging...

Claims

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Application Information

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IPC IPC(8): C12P19/20C12P19/18C12P19/16C12P19/14C12P19/12C12P19/02C12P19/00C07H3/06C07H1/06C07H3/04C07H3/02
CPCC07H1/06C07H3/02C07H3/04C07H3/06C12P19/00C12P19/02C12P19/12C12P19/14C12P19/16C12P19/18C12P19/20
Inventor 岳英李政雄陈建华杜德志
Owner 安得膜分离技术工程(北京)有限公司