Method for breeding rape recessive nuclear sterile temporary maintainer line and homozygous sterile line with same genetic background
A technique of recessive sterility and temporary protection, applied in the field of Brassica napus breeding, can solve problems such as poor uniformity and large genetic differences, and achieve the effect of ensuring purity and simplifying production procedures
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Embodiment 1
[0027] Example 1 Under the condition of heat shock treatment, the sterile plants of rapeseed recessive genital sterility realize selfing and reproduction
[0028] (1), the two types of HYZ1-AB (genotype is Ms3ms3ms4 b ms4 b +ms3ms3ms4 b ms4 b ) and full sterile line HYZ1A (genotype ms3ms3ms4 b ms4 c ) is planted in the field under normal conditions;
[0029] (2), in the initial stage of budding (such as figure 1 shown), select sterile plants from HYZ1-AB and HYZ1A, dig out the soil with a shovel, transplant them into pots, and then move them into the greenhouse;
[0030] (3) After 3 days of transplantation, the sterile plants survived, and they were placed in an artificial climate chamber to undergo heat shock treatment under the following conditions: 16 hours of light, 8 hours of darkness, 4 hours of treatment at 40°C under light conditions, and the rest The time and temperature are controlled at 20°C; continuous treatment for 10 days under the above light and temperat...
Embodiment 2
[0035] The selected line of embodiment 2 is Ms3Ms3ms4 c ms4 c Breeding process at the time of genotype
[0036] Transplantation process such as Figure 4 As shown, the specific steps are as follows:
[0037] (1), with temporary insurance system (ms3ms3ms4) c ms4 c ) and the sterile line in the homozygous dimorphic line HYZ1-AB (ms3ms3ms4 b ms4 b ) is the female parent and Zhongshuang 11 is crossed, and F is harvested 1 seed;
[0038] (2), field planting F 1 , select a single plant at the initial flowering stage for bagging isolation selfing, and obtain F 2 seed;
[0039] (3), field planting F 2 Groups, inspect each F in the early stage of budding 2 Fertility segregation of populations, F produced by crossing Zhongshuang11 with homozygous sterile strains 2 The segregation ratio of sterile and fertile plants in the population is close to 3:13, and the F 2 The population is fully fertile, so the genotype of Zhongshuang11 is preliminarily determined to be Ms3Ms3Ms4 ...
Embodiment 3
[0050] The selected line of embodiment 3 is Ms3Ms3ms4 b ms4 b Breeding process at the time of genotype
[0051] Transplantation process such as Figure 4 As shown, the specific steps are as follows:
[0052] (1), with temporary insurance system (ms3ms3ms4) c ms4 c ) and the sterile line in the homozygous dimorphic line HYZ1-AB (ms3ms3ms4 b ms4 b ) is the female parent and Xiangyou 15, and the F 1 seed;
[0053] (2), field planting F 1 , select a single plant at the initial flowering stage for bagging isolation selfing, and obtain F 2 seed;
[0054] (3), field planting F 2 Groups, inspect each F in the early stage of budding 2 Fertility segregation of populations, F 2 The segregation ratio of sterile plants and fertile plants in the population is 1:3, and the F produced by crossing with the temporary insurance line 2 The segregation ratio of sterile and fertile plants in the population was 3:13, so the genotype of Xiangyou 15 was determined to be Ms3Ms3ms4 b ms4 ...
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