A silk fibroin/gelatin stent material based on three-dimensional printing and a preparing method thereof

A fibroin, three-dimensional printing technology, applied in the fields of additive processing, medical science, prosthesis, etc., can solve the problems of biocompatibility and green degradable properties of scaffold materials, increase process difficulty and material cost, etc., and achieve excellent Biocompatibility and bioactivity, good effect of biocompatibility and bioactivity

Inactive Publication Date: 2017-08-15
YANCHENG INST OF IND TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen from the above existing technologies that at present, there are many studies on the preparation of scaffold materials using silk protein and gelatin as raw materials combined with 3D printing technology or freeze-drying technology, but most of them need to add cross-linking agents or curing agents to promote the curing and molding of materials. The presence of additives and curing agents not only increases the process difficulty and material cost, but also affects the biocompatibility and green degradability of scaffold materials.

Method used

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  • A silk fibroin/gelatin stent material based on three-dimensional printing and a preparing method thereof
  • A silk fibroin/gelatin stent material based on three-dimensional printing and a preparing method thereof
  • A silk fibroin/gelatin stent material based on three-dimensional printing and a preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Add the degummed silk fibers into the calcium chloride-ethanol-water ternary solution with a molar ratio of 1:2:8, heat and stir at 70°C until completely dissolved, filter, dialyze and concentrate to obtain a mass fraction of 3% fibroin solution, add gelatin to the fibroin solution, mix evenly to form a fibroin / gelatin composite hydrogel, wherein the mass ratio of the fibroin solution to the gelatin in the fibroin / gelatin mixed solution is 100:0.

[0039] (2) In the MIMICS software, a scaffold model with a cyclic cross network structure was established, and a polystyrene petri dish was used as a scaffold receiving device. The fibroin / gelatin composite hydrogel was placed in the syringe of the 3D printer, and the needle was adjusted. The distance between the needle in the cylinder and the receiving device is 0.5mm, place the receiving device on the temperature control device, adjust the temperature of the temperature control device to 4°C, and set it aside.

[0040] ...

Embodiment 2

[0043] (1) Add the degummed silk fibers into the calcium chloride-ethanol-water ternary solution with a molar ratio of 1:2:8, heat and stir at 80°C until completely dissolved, filter, dialyze and concentrate to obtain a mass fraction of 8% fibroin solution, add gelatin to the fibroin solution, mix evenly to form a fibroin / gelatin composite hydrogel, wherein the mass ratio of the fibroin solution to the gelatin in the fibroin / gelatin mixed solution is 70:30.

[0044] (2) In the MIMICS software, a scaffold model with a cyclic cross network structure was established, and a polystyrene petri dish was used as a scaffold receiving device. The fibroin / gelatin composite hydrogel was placed in the syringe of the 3D printer, and the needle was adjusted. The distance between the needle in the cylinder and the receiving device is 0.5mm, place the receiving device on the temperature control device, adjust the temperature of the temperature control device to 4°C, and set it aside.

[0045] ...

Embodiment 3

[0048] (1) Add the degummed silk fibers into the calcium chloride-ethanol-water ternary solution with a molar ratio of 1:2:8, heat and stir at 75°C until completely dissolved, filter, dialyze and concentrate to obtain a mass fraction of 5% fibroin solution, add gelatin to the fibroin solution, mix evenly to form a fibroin / gelatin composite hydrogel, wherein the mass ratio of the fibroin solution to the gelatin in the fibroin / gelatin mixed solution is 50:50.

[0049] (2) In the MIMICS software, a scaffold model with a cyclic cross network structure was established, and a polystyrene petri dish was used as a scaffold receiving device. The fibroin / gelatin composite hydrogel was placed in the syringe of the 3D printer, and the needle was adjusted. The distance between the needle in the cylinder and the receiving device is 0.5mm, place the receiving device on the temperature control device, adjust the temperature of the temperature control device to 4°C, and set it aside.

[0050] ...

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Abstract

A silk fibroin/gelatin stent material based on three-dimensional printing and a preparing method thereof are provided. The method includes adding gelatin into a silk fibroin solution to form silk fibroin/gelatin composite hydrogel, extruding the silk fibroin/gelatin composite hydrogel from a syringe needle of a three-dimensional printer, printing according to a stent model having a periodically cyclical cross-shaped network structure with the hydrogel directly curing and molding in a receiving device the temperature of which is 4 DEG C at the same time, and finally performing freeze-drying treatment. The method is simple. Printing and curing are performed simultaneously. The production efficiency is high. No chemical crosslinking agent or curing agent is used. The method and the material are green. Inherent characteristics of raw materials are not influenced. The porosity of the prepared stent material is high and the pore size is proper. The stent material is suitable for cell proliferation and differentiation, has good biocompatibility and biological activity, and meets using requirements of stents.

Description

technical field [0001] The invention belongs to the technical field of textile materials, and in particular relates to a three-dimensional printing-based silk protein / gelatin scaffold material and a preparation method thereof. Background technique [0002] Since the 1970s, porous scaffold tissue engineering has developed into an important part of the biomedical field. The increased Zengcheng tissue cells are planted on the porous scaffold to form a cell scaffold complex, which can undergo cell proliferation and differentiation in the patient's body, degrade the scaffold, and form tissues or organs that adapt to its own function and shape after a period of time , so as to achieve the purpose of repairing tissues and organs. With the continuous development of science and technology, people have more and more requirements for the complex structure and specific properties of porous scaffolds. Traditional processing methods are difficult to meet the requirements of manufacturing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/56A61L27/58A61L27/50A61L27/26B33Y10/00B33Y80/00
CPCA61L27/26A61L27/50A61L27/52A61L27/56A61L27/58B33Y10/00B33Y80/00C08L89/00
Inventor 王曙东樊理山刘华
Owner YANCHENG INST OF IND TECH
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