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Preparation method of multi-layer absorbable biological membrane

A biofilm and collagen film technology, applied in medical science, prosthesis, etc., can solve problems such as insufficient degradation rate of mechanical properties, and achieve the effects of preventing cerebrospinal fluid leakage, saving operation time, and preventing adhesion.

Active Publication Date: 2017-08-18
TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, type I collagen plays a crucial role in the regeneration and repair of dura mater. However, the existing technology is still insufficient in terms of mechanical properties, whether it is sutured, degradation rate, etc., and needs further improvement and optimization.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0057] The preparation method of multi-layer absorbable biofilm provided by the invention comprises the following steps:

[0058] Step 1: Prepare chondroitin sulfate acetic acid solution and collagen acetic acid swelling solution;

[0059] Step 2: adding chondroitin sulfate acetic acid solution to the collagen acetic acid swelling solution to obtain a collagen-chondroitin sulfate slurry, and stirring the solution uniformly and vacuumizing it during the preparation process;

[0060] Step 3: The collagen chondroitin sulfate solution is vacuum freeze-dried for the first time to obtain a collagen film with a three-dimensional structure;

[0061] Step 4: Press the collagen film into a dense collagen film;

[0062] Step 5: Pour the collagen chondroitin sulfate solution into a freeze-dried dish covered with a dense collagen film or brush a layer of uniform collagen-chondroitin sulfate slurry on the dense collagen layer, and place the collagen film evenly on the dense collagen layer,...

Embodiment 1

[0102] Preparation of Type I Collagen Swelling Solution

[0103] Thaw high-purity type I collagen extracted from bovine Achilles tendon, dissolve 3 grams of collagen in 500 ml of 0.5% acetic acid solution, and stir for 1-2 hours to obtain collagen acetic acid swelling solution.

[0104] Preparation of collagen-chondroitin sulfate slurry

[0105] Slowly add 400 ml of chondroitin sulfate acetic acid aqueous solution with a concentration of 0.4% to the above-mentioned collagen acetic acid swelling solution, the stirring speed is 15000-20000 rpm, the stirring time is 1-2 hours, vacuumize to remove bubbles, and obtain collagen-sulfuric acid Chondroitin Serum.

[0106] Preparation of Dense Collagen Membrane

[0107] Pour 150 ml of the above-mentioned collagen-chondroitin sulfate slurry into a stainless steel freeze-drying tray with a thickness of 4±2 mm, and freeze-dry by a special gradient cooling-drying method to obtain a collagen film. Press the collagen film into a dense coll...

Embodiment 2

[0118] Preparation of Type I Collagen Swelling Solution

[0119] Thaw high-purity type I collagen extracted from bovine Achilles tendon, dissolve 4 grams of collagen in 600 ml of 0.5% acetic acid solution, and stir for 1-2 hours to obtain a collagen acetic acid swelling solution.

[0120] Preparation of collagen-chondroitin sulfate slurry

[0121] Slowly add 500 ml of chondroitin sulfate acetic acid aqueous solution with a concentration of 0.4% to the above-mentioned collagen acetic acid swelling solution, the stirring speed is 15000-20000 rpm, the stirring time is 1-2 hours, vacuumize to remove bubbles, and obtain collagen-sulfuric acid Chondroitin Serum.

[0122] Preparation of Dense Collagen Membrane

[0123] Pour 150 ml of collagen-chondroitin sulfate slurry into a stainless steel freeze-drying tray with a thickness of 4±2 mm, and freeze-dry by gradient cooling and drying to obtain a collagen film. Press the collagen membrane Type I into a dense collagen layer with a th...

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Abstract

The invention relates to a preparation method of a multi-layer absorbable biological membrane. The method comprises the steps of preparing a chondroitin sulfate acetic acid solution and a collagen acetic acid swelling solution; adding the chondroitin sulfate acetic acid solution to the collagen acetic acid swelling solution to obtain collagen-chondroitin sulfate slurry; carrying out first vacuum freeze-drying on a collagen chondroitin sulfate solution to obtain a collagen membrane; pressing the collagen membrane into a compact collagen membrane; pouring the collagen chondroitin sulfate solution into a freeze-drying plate paved with the compact collagen membrane or directly adhering a compact collagen layer to the collagen membrane through the collagen-chondroitin sulfate slurry, carrying out second vacuum freeze-drying to obtain a compound collagen membrane; crosslinking the compound collagen membrane; and sterilizing the crosslinked compound collagen membrane to obtain the multi-layer absorbable biological membrane. The multi-layer absorbable biological membrane formed by a loose layer and the compact layer is prepared from high-purity I-type collagen as a raw material; and the multi-layer absorbable biological membrane has good biocompatibility, and is nontoxic and free of antigen reaction.

Description

technical field [0001] The invention relates to the technical field of tissue engineering and regenerative medical materials, in particular to a repair operation for dura mater defects, including supratentorial and cerebral hemisphere surgery, subtentorial and posterior fossa surgery, skull base minimally invasive surgery, and keyhole surgery Preparation of layered absorbable biofilms for surgical repair of cerebrospinal fluid leaks. Background technique [0002] The dura mater is an important tissue structure on the surface of the brain tissue, and it is an important barrier to protect the brain tissue. The integrity of the dura mater is very important for patients undergoing brain surgery. Meningeal repair materials are of great significance for rebuilding the integrity of the dura mater, protecting brain tissue, and preventing complications such as cerebrospinal fluid leakage, intracranial infection, encephalocele, and epilepsy. Trauma, tumor erosion, and the surgical pr...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/24A61L27/20A61L27/50A61L27/56A61L27/58
CPCA61L27/20A61L27/24A61L27/50A61L27/56A61L27/58C08L5/08
Inventor 康承桂王旭强李晓静
Owner TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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