Automatic SVF (Stromal Vascular Fraction) cell separating device and method thereof

Abstract

The invention discloses an automatic SVF (Stromal Vascular Fraction) cell separating device. The device comprises a rack, wherein a liquid feeding component, a main body tank, a filter device and a power device are arranged on the rack; the liquid feeding component and the main body tank are connected through a guide pipe; and the main body tank and the power device are connected through two guide pipes, one guide pipe directly connected with the power device and then connected with a collector and the other guide pipe is connected with the power device after passing through the filter device and then connected with a waste liquid cylinder. By means of the device, SVF cells can be full-automatically collected under the conditions of sealing and asepsis in whole process. The device is reasonable in structural design, convenient and simple in use, controllable in separation process and high in separation rate and is capable of greatly saving the separation time, increasing the separation efficiency and guaranteeing the activity of adipose-derived stem cells. Accordingly, the invention also discloses a corresponding application method and by applying the automatic separating device and the method thereof, the management and the industrialization of large-scale separation and extraction of the SVF cells can be realized.

Description

technical field [0001] The invention belongs to the technical field of stem cell separation and extraction, and in particular relates to an automatic SVF cell separation device and a method thereof. Background technique [0002] Adipose tissue has become a very attractive cell source in tissue engineering and regenerative medicine because it is rich in stem / progenitor cell populations and can be easily harvested. The primary cultured cell population isolated from human lipoaspirate is called adipose-derived vascular stromal fraction (SVF). SVF in adipose tissue contains different ratios of mesenchymal progenitor / stem cell populations, such as adipocytes, endothelial cells, smooth muscle cells, fibroblasts, T cells, pericytes, and M2 macrophages in addition to adipose progenitor / stem cells . After induction, adipose stem cells isolated from SVF can be easily expanded in vitro and have the potential to differentiate into cells of different lineages. They have broad clinical ...

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Problems solved by technology

[0003] However, in the current SVF cell separation and extraction technology, in an open separation and extraction environment, it is easy to cause pollution; and the process is complex and requires more instruments, and the extraction steps are cumbersome and uncontrollable. For example, fat tissue needs to be chopped manually, which is not convenient for clinical care. personnel use

In addition, the traditional way of separating adipose stem cells is to filter the digested tissue suspension with a filter. During the filtration process, the connective tissue gradually blocks the filter pores, resulting in slow filtration and prolonged filtration, which affects the activity of primary cells.

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