Dydrogesterone degrading bacterium and application thereof

A technology of dehydroprogesterone and degrading bacteria, applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., to achieve the effect of fast degradation rate

Active Publication Date: 2017-08-29
GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports on the degradation of dehydroprogesterone by bacterial strains at home and abroad, and relevant research is urgently needed

Method used

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  • Dydrogesterone degrading bacterium and application thereof
  • Dydrogesterone degrading bacterium and application thereof
  • Dydrogesterone degrading bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Domestication, isolation and identification of dehydroprogesterone-degrading bacteria DGT2

[0021] The source of bacteria was collected from the flushing water of a large-scale pig farm in Foshan City, Guangdong Province. Collect sufficient flushing water, mix it and divide it into three 1L Erlenmeyer flasks, 500mL each; add dehydroprogesterone standard samples to make the concentration of dehydroprogesterone in the wastewater reach 500μg / L; seal with multi-layer gauze Then acclimatize and cultivate at 25°C and 150rpm in the dark; about 1 week later, add a higher concentration of dehydroprogesterone; repeat this method for acclimation several times, until 60 days, the concentration of dehydroprogesterone is 5mg / L.

[0022] After the acclimation is stopped, take 100 μL of the acclimatization solution on the MH agar medium, and spread the plate with the triangle coating method; after the plate is coated several times, select bacteria with different shapes, col...

Embodiment 2

[0025] Example 2: Degradation of high concentration dehydroprogesterone by bacterial strain DGT2 in MH broth medium

[0026] Prepare MH broth medium, sterilize and cool to room temperature, subpackage into 1L Erlenmeyer flasks under aseptic conditions, add dehydroprogesterone to make the initial concentration reach 5mg / L, the experiment is divided into two groups: (1) In the bacteria experimental group, the bacterial strain DGT2 was inoculated into the MH broth medium, and mixed evenly; (2) in the sterile control group, the DGT2 strain was not inoculated, and sodium azide (final concentration of 0.5% was added to the broth medium) ). All Erlenmeyer flasks were sealed with multi-layered gauze, wrapped in tin foil to avoid light, and placed in a shaker (150rpm, 25°C) for cultivation; the experimental group and the control group were set in three parallels, and the concentration of dehydroprogesterone in the culture medium was continuously measured. Residue, analyze strain DGT2 ...

Embodiment 3

[0028] Example 3: Degradation of high-concentration dehydroprogesterone by bacterial strain DGT2 in pig wastewater

[0029]The wastewater was collected from the flushing water of a large-scale pig farm in Foshan City, Guangdong Province. The sterilized wastewater was divided into conical flasks under aseptic conditions, and dehydroprogesterone standard samples were added to make the dehydroprogesterone concentration in the wastewater reach 5 mg / L. The experiment was divided into two groups: (1) the bacteria experimental group, the strain DGT2 was inoculated into the sterilized pig raising wastewater, and mixed evenly; (2) the sterile control group, the DGT2 strain was not inoculated, and azide was added to the wastewater NaCl (0.5% final concentration). All Erlenmeyer flasks were sealed with multi-layer gauze, wrapped in tin foil to avoid light, and placed in a shaker (150rpm, 25°C) for cultivation; the experimental group and the control group were set up in 3 parallels, and ...

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Abstract

The invention discloses a dydrogesterone degrading bacterium and application thereof and belongs to the technical field of biological treatment of environmental organic pollutants. The dydrogesterone degrading bacterium is found from scouring water from a large-scale pig farm in Foshan, Guangdong, is prepared via manual enrichment culture and isolation and purification, and is named Bacillus sp. DGT2, which is collected on 2 Nov., 2016, under GDMCC NO: 60101, in Guangdong Culture Collection Center of Guangdong Institute of Microbiology with the address of Level 5, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The dydrogesterone degrading bacterium is efficient in degrading dydrogesterone and is applicable to the degradation of single dydrogesterone contamination and the degradation of mixture contaminants of various steroid hormones, and a new germplasm resource is provided for the biological treatment of farming wastewater containing steroid hormones, such as dydrogesterone.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and in particular relates to a dehydroprogesterone-degrading bacterium and its application. Background technique [0002] Dehydroprogesterone is a synthetic progestin, which has stronger selectivity and bioavailability than natural progesterone, and is widely used in the treatment of human diseases (such as infertility, threatened abortion, recurrent abortion, menstrual disorders, Endometriosis, etc.) and large-scale livestock and poultry breeding (such as inducing synchronous ovulation, preventing abortion and promoting growth, etc.). Due to the imperfection of wastewater treatment facilities and the limited effect of traditional treatment processes, dehydroprogesterone substances are generally detected in the receiving environment and groundwater around sewage treatment plants and large-scale pig farms, with concentrations ranging from 2 to 2188 ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F101/34C02F103/20
CPCC02F3/34C02F2101/34C02F2103/20C12N1/205C12R2001/07
Inventor 刘双双应光国陈军刘有胜张晋娜胡立新
Owner GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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