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Preparation of copper ion complexing type fluorescent molecule probe, and application of copper ion complexing type fluorescent molecule probe in detection of salicylic acid

A fluorescent molecular probe and copper ion technology, applied in the field of chemical analysis and detection, can solve the problems of harsh experimental conditions, inability to respond to the biological environment of cells, and inability to detect in vivo, achieving high sensitivity and fast response time

Active Publication Date: 2017-09-01
GUIZHOU UNIV
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Problems solved by technology

Among the existing methods for detecting SA concentration, there are mainly ultraviolet spectrophotometry, fluorescence spectrophotometry, colorimetry, electrochemical analysis, gas chromatography and high performance liquid chromatography, etc., but these methods cannot be detected in vivo. Detection, can not reflect the role of SA and conduction path
In the study of SA action sites, there are mainly surface plasmon resonance technology (SPR) and isotope tracer method, but surface plasmon resonance technology is mainly limited to in vitro protein experiments, which cannot reflect the biological environment of cells, while isotope tracer method It is necessary to use isotope-labeled exogenous SA, the experimental conditions are harsh and cannot reflect the production and conduction process of endogenous SA, and the above methods cannot detect endogenous salicylic acid in real time

Method used

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  • Preparation of copper ion complexing type fluorescent molecule probe, and application of copper ion complexing type fluorescent molecule probe in detection of salicylic acid
  • Preparation of copper ion complexing type fluorescent molecule probe, and application of copper ion complexing type fluorescent molecule probe in detection of salicylic acid
  • Preparation of copper ion complexing type fluorescent molecule probe, and application of copper ion complexing type fluorescent molecule probe in detection of salicylic acid

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Embodiment

[0025] 1. Probe 1-Cu 2+ preparation of

[0026] Weigh 0.0037g curcumin in a 10mL volumetric flask, dissolve and settle to volume with N,N-dimethylformamide: water (7:3, v / v) mixed solution to obtain a concentration of 10 -3 M curcumin stock solution, weighed 0.0013g Cu(NO 3 ) 2 In a 10mL volumetric flask, dissolve and make to volume with a mixed solution of N,N-dimethylformamide: water (7:3, v / v) to obtain a concentration of 10 -3 Cu(NO 3 ) 2 Stock solution, and then mix the above two solutions at a volume ratio of 1:1 to obtain probe 1-Cu 2+ .

[0027] 2. Probe 1-Cu 2+ Selectivity to salicylic acid

[0028] In the system of N,N-dimethylformamide: water (7:3, v / v), to 1-Cu 2+ (10μM) were added 20 times the equivalent of catechol (Catechol), o-toluic acid (2-MeBA), benzoic acid (BA), acetylsalicylic acid (AcetylSA), p-hydroxybenzoic acid (4- OHBA), phenol (phenol), m-hydroxybenzoic acid (3-OHBA), o-methoxybenzoic acid (2-MeOBA), methyl salicylate (MeSate), salicylalde...

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Abstract

The invention relates to a copper ion complexing type fluorescent molecule probe which recognizes salicylic acid in a manner of fluorescence starting; and the structure of the probe is shown as that in the description. The probe has relatively good selectivity to salicylic acid and is not affected by catechol, o-methylbenzoic acid (2-MeBA), benzoic acid (BA), acetylsalicylic acid (AcetylSA), p-hydroxybenzoic acid (4-OHBA), phenol, m-hydroxybenzoic acid (3-OHBA), o-methoxybenzoic acid (2-MeOBA), salicylic acid methyl ester (MeSate), salicylaldehyde and other salicylic acid analogues. The probe has the characteristics of high sensitivity, high response speed and low detection limit in the process of recognizing SA. Fluorescence images of the probe for recognizing salicylic acid in kidney cells of mouse are obtained by a two-photon confocal fluorescence microscope.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to the application of a copper ion complexed fluorescent molecular probe in the detection of salicylic acid. Background technique [0002] Salicylic acid (SA) is a signal molecule necessary for inducing plant systemic acquired resistance (SAR), and plays a very important role in mediating plant SAR signal transduction. In recent years, since the role of SA in stimulating the mechanism of plant SAR has attracted much attention, it has become very urgent to find a method that can detect endogenous SA in plants. Among the existing methods for detecting SA concentration, there are mainly ultraviolet spectrophotometry, fluorescence spectrophotometry, colorimetry, electrochemical analysis, gas chromatography and high performance liquid chromatography, etc., but these methods cannot be detected in vivo. The detection cannot reflect the action location and conductio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C49/255C07C45/77C09K11/06G01N21/64
CPCC07C45/77C07C49/255C09K11/06C09K2211/1007C09K2211/188G01N21/6428G01N21/6486
Inventor 杨松陈冲王培义杨林林吴志兵李虎薛伟
Owner GUIZHOU UNIV
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