Method for producing fucoxanthin by culturing smooth nitzia under light
A fucoxanthin and light culture technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the optimization of uncultivated conditions, the difficulty of industrial application, and the low yield of fucoxanthin with biomass concentration. and other problems to achieve the effect of reducing production costs, shortening production cycles, and reducing pollution risks
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Embodiment 1
[0051] Autotrophic culture of Nitzschia smoothis
[0052] Nitzschia laevis UTEX 2047 (purchased from the Microalgae Collection of the University of Texas at Austin, Culture Collection of Algae at The University of Texas Austin, UTEX for short) was selected as the production strain.
[0053] The process steps are as follows:
[0054] A, inoculating the Nitzschia smoothis that produces strain after the activation is placed in the aseptic seed culture medium that is placed in the shake flask and cultured heterotrophically for 3 days to make a seed liquid, and the Nitzkiplano cells in the seed liquid are in the logarithmic growth phase;
[0055] B. Using a 250mL Erlenmeyer flask as a culture vessel, transfer to 100mL of fermentation medium and sterilize it. The seed solution in step A is inserted into the sterile fermentation medium according to the inoculation amount of 10% by volume ratio, and the fermentation liquid is prepared by shaking table autotrophic culture, and the cul...
Embodiment 2
[0062] Polyculture culture of Nitzschia smoothis
[0063] The difference between Example 2 and Example 1 is that the seed medium and fermentation medium in Example 2 also contain the following raw materials: 5 g / L of glucose, and the rest of the content is the same as that of Example 1.
[0064] The effect analysis of embodiment 1,2:
[0065] Compared under autotrophic and polyculture conditions, Nitzschia smoothis, polyculture culture growth state is better, and polyculture biomass is up to 2.68g / L, and autotrophy is only 0.70g / L (see figure 1 ). like figure 2 , the autotrophic fucoxanthin content is 1.0%, and the polyculture is up to 1.2%. It was calculated that the yield of fucoxanthin under polyculture conditions was higher than that under autotrophy, and the optimal yield was 4.59 mg / (L·d).
Embodiment 3
[0067] Polyculture culture of Nitzschia smoothis
[0068] The difference between Example 3 and Example 2 is that the seed medium and the fermentation medium also contain the following raw materials: glucose 20g / L, and the production strain is Nitzschia laevis (Nitzschia laevis) CCMP 559 (purchased from U.S. Ocean Micro Algae and Microbiota Collection Center, National Center for Marine Algae and Microbiota, NCMA for short), and all the other contents are the same as in Example 2.
[0069] The effect analysis of embodiment 3:
[0070] The growth state of the production strain in this embodiment is better, and the biomass is up to 2.88g / L. The content of autotrophic fucoxanthin is 0.9%. It is calculated that the yield of fucoxanthin produced by fermentation of the production strains in this example is higher than that of autotrophy, and the optimal yield is 4.32 mg / (L·d).
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Abstract
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