Method for detecting S<2> based on calcein-rhodamine B-gold ion system

A technology of calcein and gold ions, applied in the field of detection of S2-based on calcein-rhodamine B-gold ion system, can solve the problems of low sensitivity, high cost, time-consuming, etc.

Active Publication Date: 2017-09-01
ANHUI NORMAL UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] It is well known that sulfide ions (S 2- ) are widely distributed in natural water and wastewater, sulfide ions (S 2- ) concentration is an important environmental index and aquatic pollution index, so far, there are many traditional methods for detecting sulfur ions (S 2- ) concentration, the traditional methods include electrochemical, chromatography, spectrometry, titration and gravimetric analysis, etc., these detection methods are applied to sulfide ion (S 2- ) in the detection report, although the methods for these sulfide ions have certain selectivity, but these methods are not good for sulfide ions (S 2- ) detection is very complex and time-consuming, in addition, these detection methods are often high cost, low sensitivity

Method used

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  • Method for detecting S&lt;2&gt; based on calcein-rhodamine B-gold ion system
  • Method for detecting S&lt;2&gt; based on calcein-rhodamine B-gold ion system
  • Method for detecting S&lt;2&gt; based on calcein-rhodamine B-gold ion system

Examples

Experimental program
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Embodiment 1

[0042] A Calcein-Rhodamine B-Au(III) System Based on the Detection of S 2- The method, described method comprises the following steps:

[0043] (1) Take 100μL of 10 -5 mol / L of calcein and 1000μL 10 -4 mol / L rhodamine B in 10mL test tube, add 200μL 10 -4 mol / L chloroauric acid solution, then add 100 μL of PBS buffer solution, adjust the pH to 5.9, stir and react for 25 minutes, and prepare the aqueous solution of calcein-rhodamine B-Au(III);

[0044] (2) Add 0 μL, 1 μL, 8 μL, 30 μL, 50 μL, 80 μL, 130 μL, 160 μL, 190 μL, 220 μL, 240 μL, 260 μL to the above-mentioned aqueous solution of calcein-rhodamine B-Au(III) at a concentration of 10 -3 mol / L of S 2- solution, diluted to 10 mL with deionized water, to obtain S 2- The final concentrations of the solutions were 0, 0.1 μmol / L, 0.8 μmol / L, 3 μmol / L, 5 μmol / L, 8 μmol / L, 13 μmol / L, 16 μmol / L, 19 μmol / L, 22 μmol / L, 24 μmol / L, 26μmol / L, mixed and stirred for 25min to obtain the detection system;

[0045] (3) The excitation w...

Embodiment 2

[0049] Calcein@RhB / Au(III) system to S 2- selective experiment

[0050] Add some other common anions, such as SO 3 2- 、HPO 4 2- 、Ac - , NO 2 - , F - , SO 4 2- , NO 3 - , Cl - , I - , CO 3 2- For the selectivity test, measure the fluorescence intensity, and make a histogram with (F0-F) / F0 as the ordinate and the ion species as the abscissa, and then add these ions to the Calcein@RhB / Au(III) system Add S to 2- , measure its fluorescence intensity, and make a histogram with (F0-F) / F0 as the ordinate and ion species as the abscissa. In this test, the final concentration of other anions is 120 μmol / L, S 2- The final concentration was 6 μmol / L, and other experimental conditions were the same as in Example 1.

[0051] The result is as Figure 9 It can be seen from the figure that when other anions of the same concentration are added to the Calcein@RhB / Au(III) fluorescent system, the fluorescence intensity of the Calcein@RhB / Au(III) system is quenched by other anion...

Embodiment 3

[0053] Influence of Reaction Time on the Fluorescence Intensity of the System

[0054] The reaction time t is one of the very important parameters, and the reaction time t will affect the sensitivity of target analyte detection. In the case that other conditions are the same as in Example 1, S 2- The final concentration was 1 μmol L -1 , change the reaction time of steps (1) and (2), and test the change of the fluorescence intensity of the system under different reaction times, the results are as follows Figure 10 As shown, it can be seen from the figure that when the reaction reaches 25 minutes, the fluorescence intensity of the system tends to be stable, so the reaction time of the control system in the present invention is 25 minutes.

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Abstract

The invention discloses a method for detecting S<2-> based on a calcein-rhodamine B-gold ion system. The method comprises the following steps: adding S<2-> solutions with different volumes into aqueous solutions of calcein-rhodamine B-gold ions; testing fluorescence intensity of the system under a condition that the excitation wavelength is 450nm; realizing detection on S<2-> by carrying out ionic quenching on fluorescence of the aqueous solutions of calcein-rhodamine B-gold ions through S<2->. The method is simple in operation and has low detection limit; moreover, the detection on S<2> can be quickly and accurately realized, and interference from other ions can be eliminated.

Description

technical field [0001] The invention relates to a system based on calcein-rhodamine B-gold ion system to detect S 2- Methods. Background technique [0002] With the continuous development of my country's economic construction, environmental pollution such as air and water has seriously endangered the homeland on which human beings depend for survival. Among them, the discharge of sulfur compounds is the most serious water pollution, which has seriously threatened human health, survival and development. For the human body, although natural elemental sulfur is non-toxic and harmless to the human body, sulfur-containing compounds such as dilute sulfuric acid, sulfate, sulfurous acid and sulfite are toxic. Highly toxic, concentrated sulfuric acid will corrode human skin. The study found that sulfur (S 2- ) Waste water will not only cause the death of aquatic animals and plants, but also endanger human health, and even corrode some metal equipment stored in water bodies. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/38
Inventor 许美姣黄雨阳刘金水
Owner ANHUI NORMAL UNIV
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