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Method for preparing placenta stem cell composite fibroin dressing

A technology of placental stem cells and silk protein, which is applied in the field of preparation of placental stem cell composite silk protein dressings, can solve problems such as limiting the application of BMSCs, and achieve the effects of accelerating skin repair, slow degradation speed, and convenient material extraction

Inactive Publication Date: 2017-09-05
WUHU YANGZHAN NEW MATERIAL TECH SERVICE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies mainly focused on BMSCs derived from bone marrow, but BMSCs need to be obtained through bone marrow puncture, and the number decreases with age, which limits the application of BMSCs, and the selection and application of scaffold materials have always affected the development of this technology. problem

Method used

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Examples

Experimental program
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Embodiment Construction

[0021] Below in conjunction with embodiment the present invention is described in further detail.

[0022] A preparation method of placental stem cell composite silk protein dressing, comprising the following steps:

[0023] Step 1: Mechanically strip the placental amniotic tissue, and make the remaining villi tissue with an area of ​​10cm 2 , over 75% alcohol, then rinse with PBS containing 0.1% penicillin-streptomycin to wash away the blood stains, and cut it into 1mm 2 After the small pieces were submerged in low-glucose basal medium (L-DMEM) containing 0.1% collagenase type IV, digested at 37°C for 15-45 minutes to obtain cell tissue fluid;

[0024] Step 2: transfer the cell tissue fluid to a clean centrifuge tube after being sieved through a 200-mesh sieve, centrifuge at 1200rpm for 10min, discard the supernatant and inoculate it in low-sugar basal medium (L-DMEM) containing 10% fetal bovine serum at 37°C, 5% CO 2 After culturing in the incubator for 5 days, the medium...

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PUM

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Abstract

The invention discloses a method for preparing a placenta stem cell composite fibroin dressing, and belongs to the field of composite biological material preparation. The method comprises the following steps: performing in-vitro culture on chorionic mesenchymal stem cells (CMSCs), preparing a silk fibroin nano fiber membrane and inoculating the chorionic mesenchymal stem cells (CMSCs). The placenta stem cell composite fibroin dressing prepared by using the method is convenient in material obtaining, is not involved with problems such as social ethics, is capable of accelerating skin repairing and has good tissue repairing effects.

Description

technical field [0001] The invention belongs to the field of composite biological material preparation, and in particular relates to a preparation method of placental stem cell composite silk protein dressing. Background technique [0002] In recent years, stem cells with proliferation and differentiation potential have become good seed cells in skin tissue engineering. The research on the application of embryonic stem cells in skin tissue engineering provides a theoretical basis for the construction of tissue engineered skin in the repair of skin damage. However, there are moral and ethical issues and tumorigenicity limiting factors in the medical application of embryonic stem cells, while mesenchymal stem cells (Mesenchymal Stem Cells, MSCs), a pluripotent stem cell with multidirectional differentiation potential, are good seed cells for skin It has attracted much attention in tissue engineering research. Previous studies mainly focused on BMSCs derived from bone marrow,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/36A61L27/38A61L27/60A61L27/58
Inventor 张春松吴长应
Owner WUHU YANGZHAN NEW MATERIAL TECH SERVICE CO LTD