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Anti-tumor composition as well as preparation method and application thereof

An anti-tumor and composition technology, applied in the field of anti-tumor compositions and their preparation, can solve the problems of specificity, enzymatic properties that cannot be adapted to scale, low-cost practical application, and lack of simultaneous directional hydrolysis of Panax notoginseng saponins, etc. , to achieve the effect of significant anti-tumor effect, controllable quality and mild reaction conditions

Inactive Publication Date: 2017-09-08
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the biotransformation of saponins mainly includes microbial transformation and enzymatic transformation, but there are still the following disadvantages: (1) Usually, the transformation of microorganisms or enzymes is carried out for a certain saponin, and there is no simultaneous directional hydrolysis by a biological enzyme Several saponins rich in Panax notoginseng saponins; (2) Nooginseng saponin extracts with stronger antitumor activity have not been oriented to the anti-tumor function. Deeply processed products
(3) The biological enzyme activity, specificity, and enzymatic characteristics used cannot adapt to large-scale, low-cost practical applications

Method used

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  • Anti-tumor composition as well as preparation method and application thereof
  • Anti-tumor composition as well as preparation method and application thereof
  • Anti-tumor composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Purpose of the experiment

[0029] Comparing the transformation of Panax notoginseng saponins by β-glucosidases from different sources, three main saponins in the Panax notoginseng saponins extract with strong specificity, excellent enzymatic properties, and simultaneous directional hydrolysis were screened out, which can significantly improve the resistance of the transformed products. Tumor active beta-glucosidase.

[0030] 2. Experimental materials

[0031] 2.1 Test drugs

[0032] Panax notoginseng saponins: purity ≥ 98%, purchased from Chengdu Master Biological Technology Co., Ltd.

[0033] 2.2 Recombinant glucosidase (all are β-glucosidase from three different sources constructed in our laboratory)

[0034] GH3Thermotoga petrophila RKU-1 (hereinafter referred to as Tpebgl3);

[0035] GH3 Thermotoga thermarum DSM 5069;

[0036] GH3 Aspergillus niger NL-1.

[0037] 2.3 cells

[0038] Mouse breast cancer cell 4T1, human breast cancer cell MCF-7, mouse colon ...

Embodiment 2

[0077] 1. Purpose of the experiment

[0078] Apoptosis Detection and Western Blot Analysis of Tumor Cells Effected by Conversion Products of Panax notoginseng Saponins

[0079] 2. Experimental materials

[0080] 2.1 Test drugs

[0081] The conversion product of Panax notoginseng saponins mentioned in Example 1 after being treated with glucosidase Tpebgl3.

[0082] 2.2 cells

[0083] For the mouse breast cancer cell 4T1, see 2.3 in Example 1 for the culture method.

[0084] 3. Experimental protocol

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Abstract

The invention discloses an anti-tumor composition as well as a preparation method and an application thereof, and belongs to the technical field of anti-tumor drugs. The composition is prepared by degrading a panax notoginseng saponin extract by virtue of [beta]-glucosaccharase. The preparation method provided by the invention is mild in reaction conditions, low in amount of side products and simple in technological operations, and the finished product (the anti-tumor composition) is clear in components and controllable in quality. The prepared panax notoginseng saponin conversion product (the anti-tumor composition) is obviously better than the panax notoginseng saponin extract in effect of inhibiting proliferation of such tumors as breast cancer, liver cancer, colon cancer, lung cancer and the like.

Description

technical field [0001] The invention belongs to the technical field of anti-tumor drugs, and in particular relates to an anti-tumor composition prepared by using extreme thermostable glycosidase, a preparation method and application thereof. Background technique [0002] Panax notoginseng is a plant of the genus ginseng of the family Araliaceae. It is a precious Chinese medicinal material in my country. The rhizome is used as medicine. Saponins are the main active ingredients of Panax notoginseng. So far, more than 70 monomeric saponins have been isolated from different parts of Panax notoginseng, including ginsenosides Rb1, Rd, Re, Rg1 and notoginsenoside R1. Among them, ginsenosides Rb1, Rg1 and notoginsenoside R1 are the main components of the total saponins of notoginseng. These three components all show certain pharmacological effects in anti-fatigue, maintaining blood circulation, improving myocardial ischemia, anti-arrhythmia, sedation, anti-aging and anti-tumor. ...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61K31/704A61P35/00C12P1/00
CPCA61K31/704A61K36/258A61K2236/19C12P1/00A61K2300/00
Inventor 赵林果王靖秋房仙颖吴涛李琦裴建军
Owner NANJING FORESTRY UNIV
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