Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

miRNA, composition as well as application of composition in disease diagnosis

A mir-1262, myocardial infarction technology, applied in the field of disease diagnosis, can solve problems such as insufficient

Inactive Publication Date: 2017-09-15
QINGDAO MEDINTELL BIOMEDICAL CO LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have shown that miR-30a is related to myocardial hypertrophy, and can be used as a potential clinical value for the diagnosis of acute myocardial infarction biomarkers, but these are far from enough, more candidate molecular markers are needed for accurate clinical diagnosis and treatment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • miRNA, composition as well as application of composition in disease diagnosis
  • miRNA, composition as well as application of composition in disease diagnosis
  • miRNA, composition as well as application of composition in disease diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Collection of samples and extraction of total RNA

[0046] The peripheral blood of patients with myocardial infarction and 6 cases of healthy controls were collected in the hospital from March 2015 to September 2016. Diagnostic criteria for acute myocardial infarction: developed according to the diagnostic criteria recommended by the third "Global Unified Definition of Myocardial Infarction" in 2012. An increase and / or decrease in the level of myocardial necrosis markers (mainly troponin) was detected, exceeding the 99th percentile value of the upper limit of the reference value at least once, and accompanied by at least one of the following ischemic symptoms.

[0047] 1) Symptoms of myocardial ischemia;

[0048] 2) New ischemic ECG changes;

[0049] 3) Pathological Q waves appear in the electrocardiogram;

[0050] 4) Imaging evidence shows new loss of myocardial activity or new local wall motion abnormality;

[0051] 5) Coronary angiography or autopsy fou...

Embodiment 2

[0053] Example 2 Sequencing, data analysis and electronic verification

[0054] Sequencing: The miRNA was sequenced using llumina Hiseq2500 / Miseq second-generation high-throughput sequencing technology, and the data processing was completed through the processes of removing adapters, removing low-quality, and decontaminating to obtain the final data. The miRNA sequencing raw data was background-corrected by transcriptome data analysis software, and then the t-test was performed to obtain the P value, and then the Fisher test was used to combine the P values ​​to screen for differentially expressed miRNAs.

[0055] Finally, miR-942-5p, miR-1262, and miR-520c-3p were selected from the candidate differentially expressed miRNAs for later experimental verification.

Embodiment 3

[0056] Embodiment 3 Electronic Verification

[0057] Electronic verification: 3 sets of mRNA data sets (GSE48060, GSE34198 and GSE61145-GPL6884) and 2 sets of miRNA data sets (GSE61741, GSE31568) were screened from the GEO (Gene Expression Omnibus) database, and 3 sets of mRNA data sets (GSE48060, GSE34198 and GSE61145 -GPL6884) has a total of 13,680 genes. We calculated and merged the effect value by using the metaMA package and using the combined P-value method to obtain 612 (FDR1) were found. The results showed that the electronic verification results were consistent with the expression trend of the sequencing results.

[0058] The method for evaluating the efficacy of a single miRNA molecule or a diagnostic model is to establish a receiver operating characteristic (ROC) curve, and judge the ability of diagnosis by calculating the area under the curve (Area UnderCurve). The value of the area under the ROC curve is between 1.0 and 0.5. In the case of AUC>0.5, the closer the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to miRNA, composition as well as an application of composition in disease diagnosis, in particular to miR-942-5p, miR-1262 as well as the application of composition of the miR-942-5p and the miR-1262 in diagnosis of myocardial infarction. The miRNA participates in different pathological processes of cardiovascular remodeling, including myocardial hypertrophy, fibrosis, myocardial function change, angiogenesis and the like, detection of dynamic change of miRNA can possibly provide clues for production and development of diseases, clinical early intervention is further guided, and the development of diseases is effectively controlled. The researched miR-942-5p and miR-1262 are closely related with myocardial infarction, can be used separately or jointly for clinical diagnosis and prevention detection of myocardial infarction and lay a foundation for research and development of clinically related diagnosis reagents.

Description

technical field [0001] The present invention relates to the field of disease diagnosis, in particular to miRNA, compositions and their application in diagnosing diseases, and more specifically to the application of miR-942-5p, miR-1262 and their compositions in diagnosing myocardial infarction. Background technique [0002] Recent studies have shown that miRNA is involved in the regulation of transcription and expression of 30%-50% of genes, and plays a very important role in the physiological and pathological mechanisms of the cardiovascular system. Existing reports have shown that miRNAs are involved in different pathological processes of cardiovascular remodeling, including myocardial hypertrophy, fibrosis, changes in myocardial function, and angiogenesis, etc. Since changes in miRNAs are earlier than changes in genes and proteins, they are also earlier than the onset of disease symptoms. Therefore, detecting the dynamic changes of miRNA may provide clues for the occurren...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68A61K45/00A61P9/10
Inventor 李曙光孙锦云
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com