Method for preparing allinase, allicin and garlic polysaccharide from garlic slice processing wastewater
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A technology for processing wastewater and garlic polysaccharide, applied in the fields of environmental management, processing and utilization of Chinese herbal medicine resources, and sewage treatment, can solve the problems that have not been seen before, and achieve the effect of realizing full and reasonable utilization and solving the problem of environmental pollution.
Active Publication Date: 2017-09-19
WUXI HONGRUI BIOMEDICAL TECH CO LTD
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Embodiment 1
[0032] Peel garlic, weigh 30g fresh garlic cloves, slice, soak in 60g deionized water at room temperature ( figure 2 ), Escherichia coli ( image 3 ), Bacillus subtilis ( Figure 4 ) have antibacterial effect.
Embodiment 2
[0034] Take 30mL of garlic flake processing wastewater, mix it with sterilized LB medium, and make a medium with a concentration of 30% (V / V), mix well, pour it on a plate, and observe Staphylococcus aureus, Escherichia coli, subtilis after 24 hours The growth situation of bacillus finds that garlic juice all has bacteriostasis to Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and its effect is suitable with embodiment 1.
Embodiment 3
[0036] (1) Peel the garlic, weigh 100g of fresh garlic cloves, slice them, soak them in 200g of deionized water at room temperature ( Figure 5 ), the size is about 55kDa, according to the corresponding literature, the size of alliinase monomer is 53.8kDa. Therefore, alliinase was successfully isolated by ultrafiltration.
[0037] Take 1ml of alliin solution, add 1ml of enzyme solution, react in a 25ml stoppered test tube for 3 minutes, then immediately add 2ml of 10% trichloroacetic acid to terminate the reaction, add 1ml of 2,4-dinitrophenylhydrazine, keep at 25°C for 5 minutes, then add 5ml of 2.5mol / L NaOH, after 10 minutes of reaction, colorimetric at 520nm (with the reaction system of inactivated enzyme, substrate, 2,4-dinitrophenylhydrazine, and NaOH as the reference solution). It is determined that the activity of the alliinase obtained by adopting the production process of the present invention is 70 U / mg. Vitality recovery 65.32%.
[0038] (2) After the permeate treat...
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Abstract
The invention discloses a method for preparing allinase, allicin and garlic polysaccharide from garlic slice processing wastewater. The method includes steps: (1) after pre-cooling of the garlic slice processing wastewater, adopting an ultra-filtration membrane being 10,000Da in molecular weight cut-off to realize ultra-filtration condensation, and combining cut-off liquid to obtain allinase crude fluid; (2) subjecting ultra-filtration filtrate to nano-filtration condensation through a 300Da nano-filtration membrane, extracting nano-filtration concentrate with CH2Cl2 for multiple times, keeping raffinate for preparation of garlic polysaccharide at the next step, combining CH2Cl2 extraction liquid, and removing CH2Cl2 by reduced pressure distillation to obtain oily liquid mainly composed of allicin; (3) collecting CH2Cl2 raffinate obtained at the step (2), and performing spray drying after reduced pressure distillation to obtain garlic polysaccharide extract. According to the method, respective extraction of various biological active components in the garlic slice processing wastewater is realized, three valuable products are acquired, recycling of the garlic slice processing wastewater is realized, and problems of resource waste and environment pollution in a garlic slice processing process are solved.
Description
technical field [0001] The invention not only belongs to the technical field of sewage treatment and environmental treatment, but also belongs to the technical field of processing and utilization of Chinese herbal medicine resources, and relates to a method for preparing alliinase, allicin and garlic polysaccharide by using garlic chip processing wastewater. Background technique [0002] Garlic (Allium Sativam L,), also known as garlic and gourd, is a plant of the genus Allium in the family Liliaceae. It was first cultivated in countries along the Mediterranean Sea such as ancient Egypt, ancient Rome and ancient Greece. More than 100 years ago, garlic was introduced into our country, and gradually spread throughout the country. Garlic is not only a plant that can cure and prevent diseases, but also a good seasoning. [0003] For the convenience of storage, consumption and long-distance transportation, millions of tons of fresh garlic are processed into dry products such as...
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