Electrochemical sensor for detecting estradiol as well as preparation method and application of electrochemical sensor

An estradiol and electrochemical technology is applied in the field of electrochemical analysis to achieve the effects of low cost, good stability and excellent electrocatalytic activity

Active Publication Date: 2017-09-22
HENGYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If there is a document disclosing Pt / multi-walled carbon nanotubes (the detection range is 5×10 -7 ~1.5×10 -5 mol / L, the detection limit is 1.8×10 -7 mol / L; LinXQ et al., Biosens Bioelectron, 2006, 22:253-259), graphene / dicetyl phosphoric acid (detection range is 4.0×10 -7 ~2.0×10 -5 mol / L, the detection limit is 7.7×10 -8 mol / L; Janegitz BC et al., Mater Sci Eng C, 2014,37:14-19) modified electrodes to prepare electrochemical sensors for estradiol. Although the detection range and detection limit have been improved, there is still room for further improvement. space

Method used

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  • Electrochemical sensor for detecting estradiol as well as preparation method and application of electrochemical sensor
  • Electrochemical sensor for detecting estradiol as well as preparation method and application of electrochemical sensor
  • Electrochemical sensor for detecting estradiol as well as preparation method and application of electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation of embodiment 1 palladium-nitrogen doped reduced graphene oxide (Pd / N-RGO)

[0039] (1) Synthesis of nitrogen-doped reduced graphene oxide (N-RGO) by wet chemical method: add 50 mg of graphene oxide to 50 mL of water, sonicate for 10 min, adjust the pH of the dispersion to 10 with ammonia water, and then add 1 mL of hydrazine React at 85°C for 15 hours; after the reaction, centrifuge the product, wash with water until the pH of the product is neutral, then centrifuge again, and then dry the product at 60°C to obtain N-RGO;

[0040] (2) Take 5mg N-RGO and sonicate in 5mg water for 15min to form solution 1; take 2mg PdCl 2 Add 1mL water and ultrasonically disperse for 5min to form solution 2; take 10mg NaBH 4 Add 1mL of water and stir to dissolve to form solution 3;

[0041] (3) Under the condition of magnetic stirring, add solution 2 and solution 3 to solution 1 drop by drop respectively, and the addition time is controlled to be 30min, then centrifuged...

Embodiment 2

[0043] Example 2 Preparation of a chemically modified electrode for detecting estradiol

[0044] Take 2 mg of Pd / N-RGO prepared in Example 1, after ultrasonication in 5 mL of N,N-dimethylformamide for 10 min, apply it on the surface of the working electrode with a micro-sampler, and dry it under an infrared lamp for use Chemically modified electrode (also known as Pd / N-RGO modified electrode) for detecting estradiol.

[0045] The chemically modified electrode prepared in this example for the detection of estradiol was used as the working electrode, the platinum electrode was used as the counter electrode, and the Ag-AgCl electrode was used as the reference electrode to form a three-electrode system, and the three-electrode system was assembled and combined with electrochemical A workstation was connected to test the following properties of the electrodes.

[0046] For the convenience of comparison, GO / glassy carbon electrodes and N-RGO / glassy carbon electrodes were prepared a...

Embodiment 3

[0059] Embodiment 3 is used for detecting the electrochemical sensor of estradiol

[0060] The chemically modified electrode used to detect estradiol prepared in Example 2 was used as the working electrode, the platinum electrode was used as the counter electrode, and the Ag-AgCl electrode was used as the reference electrode to form a three-electrode system. After the three-electrode system was assembled, it was connected to the electrochemical workstation connected to form an electrochemical sensor.

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Abstract

The invention relates to the technical field of electrochemical analysis, particularly discloses an electrochemical sensor for detecting estradiol as well as a preparation method and application of the electrochemical sensor. A chemically modified electrode of the electrochemical sensor for detecting the estradiol is a working electrode; the preparation method of the chemically modified electrode comprises the following steps: firstly, preparing a nitrogen-doped reductive graphene oxide; secondly, taking the nitrogen-doped reductive graphene oxide (N-RGO) and dispersing the nitrogen-doped reductive graphene oxide with water to obtain a solution I; dispersing PdC12 with water to obtain a solution II; taking NaBH4, and dissolving the NaBH4 with water to form a solution III; thirdly, dropwise adding the solution II and the solution III into the solution I respectively and then standing for 30 minutes or above; carrying out centrifugation and purification to obtain palladium-nitrogen-doped reductive graphene oxide (Pd / N-RGO); fourthly, modifying the surface of the working electrode with the palladium-nitrogen-doped reductive graphene oxide to obtain the chemically modified electrode for detecting the estradiol. The chemically modified electrode and the electrochemical sensor disclosed by the invention have lower detection limit and wider detection range.

Description

technical field [0001] The invention relates to the technical field of electrochemical analysis, in particular to an electrochemical sensor for detecting estradiol and its preparation method and application. Background technique [0002] Estradiol (Estradiol) alias courtship diol, chemical formula is C 18 h 24 o 2 . It is a steroid estrogen, which has endocrine disrupting effects, can promote the growth of animals and improve the lean meat ratio of poultry and livestock, and is widely used in poultry and livestock breeding. Therefore, through the food chain, it can affect the content of estradiol in the human body, thereby affecting human health. There is evidence that certain diseases such as breast and prostate cancer are linked to estradiol intake; excess estradiol can also reduce male fertility and produce severe congenital malformations in children. In addition, estradiol and its derivatives also have a wide range of pharmacological effects. Clinically, the qualit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 李俊华许志锋刘梦琴张复兴邹欢陈悦
Owner HENGYANG NORMAL UNIV
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