Determination of fertility potential from redox potential of biological samples

A fertility and sample technology, applied in biological testing, animal delivery, measuring devices, etc., can solve problems that are not suitable for rapid and large-scale measurement

Active Publication Date: 2020-01-17
卡鲁斯生物技术公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These types of measurement and detection systems are not suitable for rapid, large-scale measurements of biological fluid samples in clinical settings for assessing or monitoring human or animal health

Method used

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  • Determination of fertility potential from redox potential of biological samples
  • Determination of fertility potential from redox potential of biological samples
  • Determination of fertility potential from redox potential of biological samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0123] Example 1: Establishment of Oxidation-Reduction Potentials in Semen and Seminal Plasma

[0124] This example demonstrates that ORP can be measured in semen and seminal plasma samples.

[0125] Male collaborators of couples concerned about infertility were recruited from a single international andrology laboratory (clinical research) or a national urology laboratory (technical research). Each participant donated a single semen sample. Semen samples were allowed to liquefy at room temperature for approximately 30 minutes according to WHO guidelines on liquefaction (5th edition, 2010). After liquefaction, the sample was divided into two fractions. One fraction was centrifuged at 300g for 7 minutes to separate the seminal plasma.

[0126] semen analysis

[0127] After liquefaction, ejaculate volume, sperm cell count, sperm concentration, morphology, and total forward motility were determined using the WHO semen quality guidelines (5th edition, 2010). Smears were staine...

Embodiment 2

[0133] Example 2: Comparison of sORP / concentration measurements using fresh and frozen samples

[0134] Semen samples were obtained and processed as described in Example 1. Then, the samples were stored at -80°C for 120 min before being thawed, allowed to reach room temperature and sORP was measured. Figure 9 The results of the analysis are shown in .

[0135] The results demonstrated that freezing samples (whether as semen or seminal plasma) did not alter sORP values ​​and further confirmed the lack of difference between semen and seminal plasma samples.

Embodiment 3

[0136] Example 3: Effect of Time on Oxidation-Reduction Potential in Semen and Seminal Plasma

[0137] Semen samples were obtained and processed as described in Example 1. sORP was measured immediately (0 min) after liquefaction. The samples were then left at room temperature for 120 minutes and retested (120 minutes). Figure 10 and 11 The results are shown in .

[0138] The results demonstrated no difference in sORP values ​​between samples tested immediately and samples measured at later time points.

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Abstract

Methods and systems for measuring and using the oxidation-reduction potential (ORP) of biological samples are provided. The system generally includes a test strip and a readout device for determining ORP. The measured ORP is then used to determine characteristics related to the fertility of the subject of the sample or derived sample. Some of the characteristics that can be measured include the quality of the semen sample, oocytes or fertilized eggs. The measured ORP values ​​can also be used to determine specific characteristics of the sperm sample, such as the morphology of the sperm, the motility of the sperm, the number of cells in the sample, and the concentration of cells in the sample. Knowledge of such characteristics and fertility potential can be used to identify individuals who could benefit from particular fertility treatments.

Description

[0001] field of invention [0002] The present invention relates to methods and devices for measuring the oxidation-reduction potential (ORP) of a fluid sample and using such ORP to determine the fertility potential of a sample and / or a subject. [0003] Background of the invention [0004] Many biological fluids, such as whole blood, plasma, serum, semen, uterine and vaginal fluids have an oxidation-reduction potential (ORP). Clinically, the ORP of such fluids provides the oxidative state of the animal. More specifically, the ORP of such fluids is related to the state of health, disease and biological processes. [0005] A redox system or redox system involves the transfer of electrons from a reducing agent to an oxidizing agent according to the following equation: [0006] [0007] where ne - equal to the number of electrons transferred. At equilibrium, the redox potential (E), or oxidation-reduction potential (ORP), is calculated according to the Nernst-Peters equatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D19/00G01N33/92G01N27/26
CPCG01N27/4168A61B10/0045A61B10/0058A61B2010/0074G16H50/30G01N33/48707
Inventor 拉斐尔.巴尔-奥尔戴维.巴尔-奥尔伦纳德.T.瑞尔金伯利.B.比贾斯塔德
Owner 卡鲁斯生物技术公司
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