Multifunctional Dizyme Clostridium and Its Application

A double-enzyme Clostridium, multifunctional technology, applied in the field of microorganisms, to achieve the effects of easy cultivation, fast growth and high hydrogen production rate
CN107217023BActive Publication Date: 2020-05-05YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
Publication Date
2020-05-05

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Abstract

The invention belongs to the technical field of microbes, and in particular relates to a multifunctional novel double-enzyme clostridium and its application in fermenting hydrogen production and increasing acetic acid production. The bacterial strain is Clostridium bifermentans EZ-1, and the preservation number is CGMCC NO.13913. The bacteria obtained in the invention can utilize glucose to produce clean fuel hydrogen and metabolites such as acetic acid and butyric acid. Adding appropriate concentrations of nano-magnetite and ferrihydrite can regulate the metabolic pathway of the bacteria to increase the production of acetic acid. Through the detection of microbial fuel cells, it was found that the strain has the ability to produce electricity, and the maximum current output density can reach 6.3mA / m 2 . This strain is the first reported case of a new multi-functional double-enzyme Clostridium, which can be applied to biomass fermentation to produce biological products such as hydrogen, acetic acid and butyric acid, and has the potential to produce electricity, so it has broad application prospects.
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Description

technical field

[0001] The invention belongs to the technical field of microbes, and in particular relates to a multifunctional novel double-enzyme clostridium and its application in fermenting hydrogen production and increasing acetic acid production. Background technique

[0002] Clostridium bienzyme is a strictly anaerobic fermentative hydrogen-producing bacterium that can produce hydrogen and volatile short-chain fatty acids from fermentation substrates such as glucose, and its Gram stain is positive.

[0003] With the increasingly prominent climate change and air pollution problems caused by the use of fossil fuels, biohydrogen has great application prospects because of its clean and renewable characteristics and high combustion heat (122kJ / g). Biohydrogen is mainly produced by hydrogen-producing microorganisms through dark fermentation. Such microorganisms mainly include Clostridium, Enterobacter, and Bacillus, among which Clostridium is easy to cultivate and has high ...

Claims

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