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Method for detecting content of multiple ingredients in astragalus membranaceus and ligustrum lucidum tonic preparation

A technology of Zhenqi strengthening and determination method, which is applied in the direction of measuring device, material separation, analysis of material, etc., and can solve the problem of single quality control system

Active Publication Date: 2017-10-10
长春康彼达科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] This preparation is composed of Ligustrum lucidum and Astragalus membranaceus. In the current quality standard, the identification and content determination items only control the quality of Astragalus membranaceus, and the quality control system is single.

Method used

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  • Method for detecting content of multiple ingredients in astragalus membranaceus and ligustrum lucidum tonic preparation
  • Method for detecting content of multiple ingredients in astragalus membranaceus and ligustrum lucidum tonic preparation
  • Method for detecting content of multiple ingredients in astragalus membranaceus and ligustrum lucidum tonic preparation

Examples

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Effect test

Embodiment 1

[0066] Example 1 of the present invention: the content determination method of salidroside, calycosin glucoside, privetin and formononetin in Zhenqi Fuzheng Granules:

[0067] (1) Chromatographic conditions and system adaptability test: Octadecylsilane silica gel as filler; acetonitrile (A)-water (B) as mobile phase, gradient elution: 0-10min, 95% (A) ~90%(A), 10~30min, 90%(A)~78%(A), 30~40min, 78%(A)~73%(A), 40~55min, 73%(A)~45 %(A); the column temperature is 35°C; the detection wavelength is 220nm; the flow rate is 0.8mL / min; the number of theoretical plates shall not be less than 3000 based on salidroside.

[0068] (2) Preparation of reference substance solution: take appropriate amount of reference substances of salidroside, acteosin glucoside, prigulin and formononetin, and add methanol to make the concentration containing salidroside 0.13mg / mL, acteosin, respectively. A solution of flavonoid glucoside 0.35mg / mL, privetin 0.2mg / mL, and formononetin 0.015mg / mL is availabl...

Embodiment 2

[0071] Example 2 of the present invention: the content determination method of salidroside, calycosin glucoside, privetin and formononetin in Zhenqi Fuzheng Capsules:

[0072] (1) Chromatographic conditions and system adaptability test: Octadecylsilane silica gel is used as filler; acetonitrile (A)-0.02% formic acid aqueous solution (B) is used as mobile phase, gradient elution: 0~10min, 95% (A) ~ 90% (A), 10 ~ 30min, 90% (A) ~ 78% (A), 30 ~ 40min, 78% (A) ~ 73% (A), 40 ~ 55min, 73% (A ) ~ 45% (A). ; 30°C; Detection wavelength 220nm; Flow rate 1mL / min;

[0073] (2) Preparation of reference substance solution: take appropriate amount of reference substances of salidroside, acteosin glucoside, prigulin and formononetin, and add methanol to make the concentration containing salidroside 0.13mg / mL, acteosin, respectively. A solution of flavonoid glucoside 0.35mg / mL, privetin 0.2mg / mL, and formononetin 0.015mg / mL is available.

[0074] (3) Preparation of the test solution: take 5...

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Abstract

The invention relates to a method for detecting the content of effective ingredients of rhodioloside, calycosin-7-glucoside, specnuezhenide and formononetin. The method is high performance liquid chromatography by using rhodioloside, calycosin-7-glucoside, specnuezhenide and formononetin as reference substances and methanol: water / 0.01-5% phosphoric acid aqueous solution / 0.01-5% formic acid aqueous solution / 0.01-5% glacial acetic acid aqueous solution in a ratio of (5-100):(95-0) or acetonitrile: water / 0.01-5% phosphoric acid aqueous solution / 0.01-5% formic acid aqueous solution / 0.01-5% glacial acetic acid aqueous solution in a ratio of (5-100):(95-0). Compared with the prior art, the method has the advantages that the content of four effective ingredients can be simultaneously detected along with comprehensive quality representation, strong specificity, high stability and accurate measurement result, the aim of effectively controlling the quality of a modified preparation can be achieved, and stability of related product quality and clinical administration safety of the preparation can be guaranteed.

Description

technical field [0001] The invention relates to a content determination method of salidroside, calycosin glucoside, privetin and formononetin in Zhenqi Fuzheng preparation, and belongs to the technical field of traditional Chinese medicine quality detection. Background technique [0002] The prescription of Zhenqi Fuzheng preparation is composed of Ligustrum lucidum and Astragalus membranaceus, which have the effects of strengthening the body, nourishing qi and nourishing yin. It has been clinically applied and proved that it is a good immune function enhancer at present, and it can improve human immune function. Protect bone marrow, adrenal cortex and liver function, suitable for the recovery of various diseases caused by wasting, chronic illness (cirrhosis, chronic atrophic gastritis, diabetes, tuberculosis, etc.), postoperative and postpartum patients; radiotherapy and chemotherapy for cancer patients During the period, it has the effect of adjuvant therapy, which can sig...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 魏晓雨姜国栋姜文月王美慧陈泓徐杰边雨曲佳乐
Owner 长春康彼达科技有限公司
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