Preparation method of multilayer assembled micron-grade mono-disperse polystyrene magnetic composite microspheres

A technology of polystyrene microspheres and magnetic composite microspheres, which is applied in the field of preparation of multilayer assembled micron-scale monodisperse polystyrene magnetic composite Problems such as wide particle size distribution of polymer microspheres

Active Publication Date: 2017-10-13
成都爱兴生物科技有限公司
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  • Application Information

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Problems solved by technology

However, the microspheres obtained by the existing method have a wide particle size distribution, which is not suitable for a wide range of applications in biomedicine, and monodispersity is an important indicator for measuring polymer magnetic microspheres, especially when applied to chemiluminescence immunology, In the field of cell

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  • Preparation method of multilayer assembled micron-grade mono-disperse polystyrene magnetic composite microspheres
  • Preparation method of multilayer assembled micron-grade mono-disperse polystyrene magnetic composite microspheres
  • Preparation method of multilayer assembled micron-grade mono-disperse polystyrene magnetic composite microspheres

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Example Embodiment

[0026] Example 1:

[0027] This embodiment provides a method for preparing multi-layer assembled micron-level monodisperse polystyrene magnetic composite microspheres, and the specific steps are as follows:

[0028] (1) Synthesis of polystyrene microspheres: Take a 250ml round bottom flask, add 3g polyvinylpyrrolidone (Mw=55000), 100mL ethanol and 10mL water, and stir to obtain a homogeneous solution. Weigh 0.08 g of azobisisobutyronitrile as an initiator and dissolve it in 8 mL of distilled styrene monomer. The styrene monomer solution in which azobisisobutyronitrile is dissolved is added to the homogeneous solution obtained above, stirred and protected by nitrogen, the reaction temperature is increased to 70° C., and the reaction is carried out at 150 rpm / min for 20 hours. After the reaction time is up, the supernatant is removed by centrifugation, the supernatant is removed, and the dispersion is repeated six times, followed by vacuum drying at 30°C for 24 hours to obtain polys...

Example Embodiment

[0034] Example 2:

[0035] This embodiment provides a method for preparing multi-layer assembled micron-level monodisperse polystyrene magnetic composite microspheres, and the specific steps are as follows:

[0036] (1) Synthesis of polystyrene microspheres: Take a 250ml round bottom flask, add 2.0g polyvinylpyrrolidone (Mw=55000) and 120mL ethanol, and stir to obtain a homogeneous solution. Weigh 0.2 g of azobisisobutyronitrile as an initiator and dissolve it in 30 mL of distilled styrene monomer. The styrene monomer solution in which azobisisobutyronitrile is dissolved is added to the homogeneous solution obtained above, stirred and protected by nitrogen, the reaction temperature is increased to 68° C., and the reaction is carried out at 150 rpm / min for 20 hours. After the reaction time is up, centrifugation is performed to remove the supernatant, and then disperse with ethanol. Repeat six times, and vacuum-dry at 30°C for 24 hours to obtain polystyrene microspheres with a parti...

Example Embodiment

[0041] Example 3:

[0042] This embodiment provides a method for preparing multi-layer assembled micron-level monodisperse polystyrene magnetic composite microspheres, and the specific steps are as follows:

[0043] (1) Synthesis of polystyrene microspheres: Take a 250ml round bottom flask, add 4.0g polyvinylpyrrolidone (Mw=55000), 40mL ethanol and 60mL ethylene glycol methyl ether, and stir to obtain a homogeneous solution. Weigh 0.3 g of azobisisobutyronitrile as an initiator and dissolve it in 30 mL of distilled styrene monomer. The styrene monomer solution in which azobisisobutyronitrile is dissolved is added to the homogeneous solution obtained above, stirred and protected by nitrogen, the reaction temperature is increased to 72° C., and the reaction is carried out at 150 rpm / min for 20 hours. After the reaction time is up, centrifugation is performed to remove the supernatant, and then disperse with ethanol, repeat six times, and vacuum dry at 30° C. for 24 hours to obtain p...

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Abstract

The invention belongs to the technical field of high molecular functional materials and provides a preparation method of multilayer assembled micron-grade mono-disperse polystyrene magnetic composite microspheres. The preparation method of the polystyrene magnetic composite microspheres comprises the following steps: preparing mono-disperse sulfo or carboxyl polystyrene microspheres with rich negative charges on surfaces by adopting dispersion polymerization; then utilizing electrostatic interaction among the mono-disperse sulfo or carboxyl polystyrene microspheres, strong cation polyelectrolyte and sodium citrate modified Fe3O4 nanoparticles to finally prepare the micron-grade mono-disperse polystyrene magnetic microspheres. According to the preparation method provided by the invention, the mono-disperse polystyrene magnetic microspheres with highly uniform grain diameter and a micron-grade grain diameter can be obtained; the application requirements in the fields of chemiluminescence immunity, cell separation, protein purification and the like can be met; the preparation method provided by the invention also has the advantages of simplicity and convenience for operation, energy saving and consumption reduction and is suitable for industrialized popularization and application.

Description

technical field [0001] The invention belongs to the technical field of polymer functional materials, and in particular relates to a preparation method for multilayer assembly of micron-level monodisperse polystyrene magnetic composite microspheres. Background technique [0002] Magnetic polymer microsphere is a new type of advanced polymer composite functional magnetic material developed in recent years. It is a composite microsphere with certain magnetic properties and special structure formed by combining magnetic inorganic particles and organic polymers by appropriate methods. Magnetic polymer microspheres have both magnetic responsiveness and surface functionality of ordinary polymer microspheres, and are widely used in many fields such as medicine, biochemistry, immunoassay and molecular biology, especially in immobilized enzymes, targeted drugs, etc. It has broad application prospects in cell separation and other aspects. [0003] At present, the methods for preparing...

Claims

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Application Information

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IPC IPC(8): C08L25/06C08K3/22C08K9/04C08J3/12C08F8/00C08F8/36C08F112/08
CPCC08F8/00C08F8/36C08J3/12C08J2325/06C08K3/22C08K9/04C08K2003/2275C08K2201/011C08L25/06C08F112/08
Inventor 程敏卓
Owner 成都爱兴生物科技有限公司
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