Method for inducing adventitious buds of jerusalem artichoke
A bud induction, Jerusalem artichoke technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low germination rate, slow progress of Jerusalem artichoke tissue culture, difficult to achieve hybrid breeding, etc., and achieve good repeatability.
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Embodiment 1
[0015] The method for inducing adventitious buds of Jerusalem artichoke in this example uses young cotyledons as explants, cultures them in dark until calluses appear, treats them with negative ion photocatalyst for 8 minutes, and then soaks them in 70% alcohol for 15 minutes. Rinse twice; inoculate on the callus medium, set the culture temperature at 22°C-26°C, and culture in dark for 15 days; transfer to the adventitious bud induction medium, set the culture temperature at 24°C-27°C, Cultured under light until adventitious buds grow; the callus culture medium is MS culture medium with 1 mg / L 6-benzylpurine, 0.15 mg / L coconut milk and 35 g / L sucrose; the adventitious bud induction medium is MS culture Add 0.7mg / L naphthaleneacetic acid, 0.5mg / L inositol, and 0.2mg / L pantothenic acid.
[0016] The preparation method of the adventitious bud culture medium in this example is: mix the ingredients in MS except agar, then use distilled water to make up the volume, adjust the pH val...
Embodiment 2
[0019] The method for inducing adventitious buds of Jerusalem artichoke in this example uses young cotyledons as explants, cultures them in dark until calluses appear, treats them with negative ion photocatalyst for 9 minutes, and then soaks them in 70% alcohol for 13 minutes. Rinse 5 times; inoculate on the callus medium, set the culture temperature at 22°C-26°C, and culture in dark for 12 days; transfer to the adventitious bud induction medium, set the culture temperature at 24°C-27°C, Cultured under light until adventitious buds grow; the callus culture medium is MS medium with 2mg / L 6-benzylpurine, 0.25mg / L coconut milk and 25g / L sucrose; the adventitious bud induction medium is MS culture Add 1mg / L naphthaleneacetic acid, 1mg / L inositol, 0.4mg / L pantothenic acid.
[0020] The preparation method of the adventitious bud medium in this example is as follows: mix the ingredients in MS except agar, then use distilled water to make up the volume, adjust the pH value to 6, divid...
Embodiment 3
[0023] The method for inducing adventitious buds of Jerusalem artichoke in this example uses young cotyledons as explants, cultures them in dark until calluses appear, treats them with negative ion photocatalyst for 10 minutes, and then soaks them in 70% alcohol for 10 minutes. Rinse 3 times; inoculate on the callus culture medium, set the culture temperature at 22°C-26°C, and culture in dark for 8 days; transfer to the adventitious bud induction medium, set the culture temperature at 24°C-27°C, Light culture until adventitious buds grow; the callus culture medium is MS medium with 3mg / L 6-benzyl purine, 0.35mg / L coconut milk and 30g / L sucrose; the adventitious bud induction medium is MS culture Add 1.3mg / L naphthaleneacetic acid, 1.5mg / L inositol, and 0.6mg / L pantothenic acid.
[0024] The preparation method of the adventitious bud medium in this example is as follows: mix the ingredients in MS except agar, then use distilled water to make up the volume, adjust the pH value t...
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