Method for inducing adventitious buds of jerusalem artichoke

A bud induction, Jerusalem artichoke technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low germination rate, slow progress of Jerusalem artichoke tissue culture, difficult to achieve hybrid breeding, etc., and achieve good repeatability.

Inactive Publication Date: 2017-10-24
河池市智汇科技咨询有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of asexual reproduction makes it difficult to obtain Jerusalem artichoke seeds, and the germination rate is extremely low, making it difficult to achieve cross breeding
[0004] With the development of modern biotechnology, more researchers use tissue culture to cultivate Jerusalem artichoke. However, it is difficult to obtain regeneration plants from Jerusalem artichoke callus. Therefore, the progress of tissue culture of Jerusalem artichoke is still slow. Propagation of axillary buds and shoot tips, in vitro mutagenesis and polyploid mutagenesis using tubers and shoot tips of Jerusalem artichoke, but the tissue culture system of Jerusalem artichoke to induce complete plants through callus has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The method for inducing adventitious buds of Jerusalem artichoke in this example uses young cotyledons as explants, cultures them in dark until calluses appear, treats them with negative ion photocatalyst for 8 minutes, and then soaks them in 70% alcohol for 15 minutes. Rinse twice; inoculate on the callus medium, set the culture temperature at 22°C-26°C, and culture in dark for 15 days; transfer to the adventitious bud induction medium, set the culture temperature at 24°C-27°C, Cultured under light until adventitious buds grow; the callus culture medium is MS culture medium with 1 mg / L 6-benzylpurine, 0.15 mg / L coconut milk and 35 g / L sucrose; the adventitious bud induction medium is MS culture Add 0.7mg / L naphthaleneacetic acid, 0.5mg / L inositol, and 0.2mg / L pantothenic acid.

[0016] The preparation method of the adventitious bud culture medium in this example is: mix the ingredients in MS except agar, then use distilled water to make up the volume, adjust the pH val...

Embodiment 2

[0019] The method for inducing adventitious buds of Jerusalem artichoke in this example uses young cotyledons as explants, cultures them in dark until calluses appear, treats them with negative ion photocatalyst for 9 minutes, and then soaks them in 70% alcohol for 13 minutes. Rinse 5 times; inoculate on the callus medium, set the culture temperature at 22°C-26°C, and culture in dark for 12 days; transfer to the adventitious bud induction medium, set the culture temperature at 24°C-27°C, Cultured under light until adventitious buds grow; the callus culture medium is MS medium with 2mg / L 6-benzylpurine, 0.25mg / L coconut milk and 25g / L sucrose; the adventitious bud induction medium is MS culture Add 1mg / L naphthaleneacetic acid, 1mg / L inositol, 0.4mg / L pantothenic acid.

[0020] The preparation method of the adventitious bud medium in this example is as follows: mix the ingredients in MS except agar, then use distilled water to make up the volume, adjust the pH value to 6, divid...

Embodiment 3

[0023] The method for inducing adventitious buds of Jerusalem artichoke in this example uses young cotyledons as explants, cultures them in dark until calluses appear, treats them with negative ion photocatalyst for 10 minutes, and then soaks them in 70% alcohol for 10 minutes. Rinse 3 times; inoculate on the callus culture medium, set the culture temperature at 22°C-26°C, and culture in dark for 8 days; transfer to the adventitious bud induction medium, set the culture temperature at 24°C-27°C, Light culture until adventitious buds grow; the callus culture medium is MS medium with 3mg / L 6-benzyl purine, 0.35mg / L coconut milk and 30g / L sucrose; the adventitious bud induction medium is MS culture Add 1.3mg / L naphthaleneacetic acid, 1.5mg / L inositol, and 0.6mg / L pantothenic acid.

[0024] The preparation method of the adventitious bud medium in this example is as follows: mix the ingredients in MS except agar, then use distilled water to make up the volume, adjust the pH value t...

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PUM

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Abstract

The invention discloses a method for inducing adventitious buds of jerusalem artichoke and relates to the technical field of jerusalem artichoke tissue culture. A callus culture medium is formed by adding 1-3mg/L 6-benzyl purine, 0.15-0.35mg/L coconut milk and 25-35g/L saccharose into a MS culture medium; an adventitious bud inducing medium is formed by adding 0.7-1.3mg/L naphthylacetic acid, 0.5-1.5mg/L inositol and 0.2-0.6mg/L pantothenic acid into the MS culture medium; the adventitious buds successfully grow at last; the induction rate of the adventitious buds is above 15.5% and the repeatability is higher.

Description

technical field [0001] The invention relates to the technical field of Jerusalem artichoke tissue culture, in particular to a method for inducing adventitious buds of Jerusalem artichoke. Background technique [0002] Jerusalem artichoke, the scientific name is Helianthus tuberosus (L.1753), also known as Jerusalem artichoke, ghost ginger, is a perennial herb, native to North America, introduced to Europe in the 17th century, and then introduced to China. Its underground tubers Rich in fructose polymers such as starch and inulin, it can be eaten, cooked or porridge, pickled pickles, dried artichoke in the sun, or used as raw materials for starch and alcohol production. Planting near houses can also beautify. [0003] There are two ways to reproduce Jerusalem artichoke, one is to form zygote seeds through double fertilization, that is, sexual reproduction, and the other is to reproduce asexually through the buds of underground tubers. The seed setting rate and seed germinati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001
Inventor 韦顺华梁胜
Owner 河池市智汇科技咨询有限公司
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