A biochemical detection system and method based on electrochemically enhanced reflection spectrum signals
A reflection spectroscopy and biochemical detection technology, applied in the field of biochemical detection systems, can solve the problems of non-specific adsorption and insufficient diffusion of the measured substance, and achieve the effects of good biological affinity, improved electrical conductivity, and improved reflection spectral response.
Active Publication Date: 2019-04-19
ZHEJIANG UNIV
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However, in the actual biological fluid detection, there will be problems such as insufficient diffusion of the analyte and non-specific adsorption.
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[0037] The present invention is described in detail below with reference to the accompanying drawings and specific embodiments, but does not limit the present invention.
[0038] The advantage of the present invention is that the optical and electrical information in the reaction process can be captured at the same time, and the key lies in the design and construction of the system. There are two parts of the system, such as figure 1 , 2 shown. The electrochemical detection system includes: a three-electrode system measurement system 1 and an electrochemical workstation 18. The three-electrode system measurement system 1 includes a silver / silver chloride reference electrode 4, a platinum wire counter electrode 5, and a nanometer working electrode. Cone array sensor 7, electrode holder 6; the three electrodes are respectively connected with the reference electrode interface 17, the counter electrode interface 15 and the working electrode interface 16 of the electrochemical wo...
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Abstract
A biochemical detection system and method based on electrochemical enhanced reflection spectrum signals are disclosed. The method includes firstly building an electrochemical detection system and a reflection spectrum detection system; then immediately preparing a gold nanocone array sensor; performing reduction and deposition of silver nanometer particles on the surface of the sensor through cyclic voltammetry; modifying the surface of the sensor with a sulfydryl-containing receptor molecule through bonding of a metal-sulfur covalent bond, with a product being used for specific detection of a target compound; building a concentration-dependent curve; and finally analyzing a compound of a concentration to be detected. The biochemical detection system and method achieve measurement and analysis of a target compound from two aspects, namely a reflection spectrum aspect and an electrochemical aspect, have advantages of high sensitivity, no need of labeling, real-time and rapid detection, and the like, and can meet requirements of electrochemical detection.
Description
technical field [0001] The invention relates to a biochemical detection system and method based on electrochemically enhanced reflection spectrum signals. Background technique [0002] Metal nanomaterials have good research and practical value in the field of biochemical sensors due to their good electrical conductivity, small size effect, quantum tunneling effect and high specific surface area. Nano-optical sensors can detect specific substances by analyzing changes in interface transmittance or refractive index, and have the advantages of real-time, fast, high sensitivity, and no need for labels. However, problems such as insufficient diffusion and non-specific adsorption of the analyte may occur in actual biological fluid detection. In order to increase the intensity of the signal and the specificity of detection, on the one hand, surface enhancement treatment and specific modification can be used. On the other hand, it is also possible to introduce directional traction...
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IPC IPC(8): G01N27/48G01N21/25
CPCG01N21/25G01N27/48
Inventor 刘清君刘京龙李爽
Owner ZHEJIANG UNIV