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Method for measuring content of calycosin-7-glucoside in lung tonifying and blood circulation promoting capsules

A technology for glucosinolates and glucosinolates, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of undocumented methods for measuring the content of glucosinolates on glucosinolates, and achieves strong specificity, good accuracy, feasibility, and precision. high effect

Active Publication Date: 2017-10-24
GUANGDONG LEIYUNSHANG PHARM CO LTD
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The first part of the 2015 edition of "Chinese Pharmacopoeia" records the prescription, preparation method and content determination method of Bufei Huoxue Capsules. It only records the content determination methods of astragaloside IV and paeoniflorin, but does not record the content determination method of acteosin glucoside.

Method used

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  • Method for measuring content of calycosin-7-glucoside in lung tonifying and blood circulation promoting capsules
  • Method for measuring content of calycosin-7-glucoside in lung tonifying and blood circulation promoting capsules
  • Method for measuring content of calycosin-7-glucoside in lung tonifying and blood circulation promoting capsules

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The selection of embodiment 1 chromatographic conditions

[0053] 1. Mobile phase

[0054] Referring to the 2015 edition of "Chinese Pharmacopoeia" in Astragalus medicinal materials [Content Determination] Calycosin glucoside, acetonitrile was used as mobile phase A, 0.2% formic acid solution was used as mobile phase B, and when the elution gradient was adjusted to the following table 2, Calycosin Both flavonoid glucoside and calycosin are well separated.

[0055] Table 2 Mobile phase elution gradient

[0056]

[0057] 2. Detection wavelength

[0058] Take the methanol solution of calycocoside glucoside and calycocoside reference substance to measure the ultraviolet absorption spectrum, both of them have large absorption at 260nm, so 260nm is selected as the detection wavelength. See attached figure 1 And attached figure 2 .

Embodiment 2

[0059] The preparation of embodiment 2 sample

[0060] 1. Preparation of reference substance solution: Take appropriate amount of actcocoside glucoside and acteocoside reference substance respectively, weigh them accurately, add 50% methanol to make a mixed solution containing 30 μg of actcocoside glucoside and 20 μg of actcocoside per 1 ml, and get ready.

[0061] 2. Preparation of the test solution: Take the content of this product, grind it finely, take about 1.2g, accurately weigh it, put it in a stoppered conical flask, add 50ml of methanol precisely, weigh it, heat and reflux for 1 hour, put Cool, weigh again, make up for the lost weight with methanol, shake well, filter, accurately measure 25ml of the subsequent filtrate, recover the solvent to dryness, add 50% methanol to the residue to dissolve, transfer to a 5ml measuring bottle, add 50% Methanol to the mark, shake well, that is.

[0062] 3. The choice of solvent for the preparation of the test solution: This test...

Embodiment 3

[0068] Embodiment 3 system suitability test

[0069] Draw the reference substance solution and the test solution into the liquid chromatograph for determination. Under this chromatographic condition, calycocoside glucoside, calycocoside and other components achieve baseline separation, and the resolution R>1.5. According to different chromatographic column theoretical plates (Hitachi column is about 13,000; Sepax column is about 15,000; Welch column is about 17,000; Thermo column is about 12,000), and the number of theoretical plates for this sample is not less than 12,000 based on calycosin glucoside. See Table 5, Table 6 and Appendix image 3 .

[0070] Table 5 System Adaptability Data (Calycosin Glucoside Reference Substance)

[0071]

[0072] Table 6 system suitability data (calycosin reference substance)

[0073]

[0074] Note: The resolution in the table is the resolution data in the precision

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Abstract

The invention provides a method for measuring content of calycosin-7-glucoside in lung tonifying and blood circulation promoting capsules. The method has good separation degree, high specificity, high precision, good repeatability, high recovery rate, good stability and measurement result accuracy. Besides, the method can also be used for measuring the content of calycosin simultaneously, so that the stability of medicine quality as well as the safety and effectiveness of clinical medication can be guaranteed.

Description

1. Technical field [0001] The invention belongs to the technical field of quality detection of traditional Chinese medicines, and relates to a method for determining the content of calycosin glucoside in Bufei Huoxue capsules. 2. Background technology [0002] Bufei Huoxue Capsules are composed of astragalus, red peony root, psoralen, tonify qi and activate blood, nourish the lung and strengthen the kidney. It is used for cor pulmonale (in remission) syndrome of qi deficiency and blood stasis. Palpitations, shortness of breath, cold limbs and fatigue, soreness of the waist and knees, cyanotic lips, pale tongue with white coating or dark purple tongue, etc. [0003] The first part of the 2015 edition of "Chinese Pharmacopoeia" records the prescription, preparation method and content determination method of Bufei Huoxue Capsules. It only records the content determination methods of astragaloside IV and paeoniflorin, but does not record the content determination method of acteo...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/884
Inventor 朱晓枭关泽明苏薇薇钱坤关敏怡谢扬辉唐清何宇辉
Owner GUANGDONG LEIYUNSHANG PHARM CO LTD
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