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Method for improving activity of anaerobic bacteria for producing butanol

An anaerobic bacteria and butanol technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as yield reduction and acid collapse, reduce by-products, alleviate stress, and avoid rapid rise high effect

Active Publication Date: 2017-10-27
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, due to the accumulation of a large amount of organic acids during the fermentation acid production period, if the pH value is not adjusted during this process, it is very likely to cause acid collapse, which will inhibit the fermentation and reduce the yield

Method used

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  • Method for improving activity of anaerobic bacteria for producing butanol
  • Method for improving activity of anaerobic bacteria for producing butanol

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specific Embodiment approach 1

[0021] Specific embodiment one: a kind of method of improving anaerobic bacteria to produce butanol activity of the present embodiment, it is carried out according to the following steps:

[0022] 1. Inoculate the butanol-producing bacteria into the sterilized liquid anaerobic fermentation medium, and shake and cultivate it to the logarithmic phase under constant temperature conditions;

[0023] 2. After the bacterial growth reaches the logarithmic phase in step 1, add the butyric acid solution to the fermentation system of step 1, so that the concentration of the butyric acid solution in the fermentation system reaches 0.6 to 1.5 g / L;

[0024] 3. After adding the butyric acid solution, intermittently add the buffering agent to the fermentation system established in step 2, and continue to vibrate and cultivate under constant temperature conditions to obtain a butanol-based fermentation liquid, that is, to complete the described process of improving the fermentation system. Bu...

specific Embodiment approach 2

[0025] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the liquid anaerobic fermentation medium described in step 1 contains a carbon source with a concentration of 40-60 g / L, 1-3 g / L yeast extract, 0.1~0.3g / L magnesium sulfate, 0.01~0.02g / L manganese sulfate, 0.01~0.02g / L ferrous sulfate, 0.01~0.02g / L sodium chloride, 0.001~0.002g / L vitamin B1, 0.3~0.7 g / L cysteine, 0.001-0.002g / L p-aminobenzoic acid, 0.0001g / L biotin and 0.1-0.2g / L resazurin. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0026] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the liquid anaerobic fermentation medium described in step one contains a carbon source with a concentration of 40-60 g / L, 2 g / L yeast extract, 0.2 g / L magnesium sulfate, 0.01g / L manganese sulfate, 0.01g / L ferrous sulfate, 0.01g / L sodium chloride, 0.001g / L vitamin B1, 0.5g / L cysteine, 0.001g / L p-amino Benzoic acid, 0.0001g / L biotin and 0.1g / L resazurin. Others are the same as in the first embodiment.

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Abstract

The invention relates to a method for improving the activity of anaerobic bacteria for producing butanol, and solves the problems of an existing butanol producing technology that the substrate cost is high; the butanol producing efficiency is low; the content of by-products is high. When butanol producing bacteria are fermented to the logarithmic phase, a certain concentration of butyric acid solution and buffer agents are externally added; after uniform mixing, shaking culture is continuously performed under the constant temperature condition; fermentation liquid containing rich butanol is obtained. The butyric acid is externally added, so that more fermentation substrates enter to the butanol synthesis metabolic pathway; the generation of by-products such as acetic acid and butyric acid of a fermentation system is reduced; the butanol yield is greatly improved; the proportion of the butanol in the fermentation liquid is increased. The two substances of butyric acid and buffer agents are simultaneously selected to be added in the logarithmic phase, so that the bacterium fermentation activity can be simulated; the pH of the fermentation system can also be stabilized not to generate an acid collapse phenomenon. The method has the advantages that the final yield of the butanol is improved; the butanol synthesis rate is also greatly improved; important significance is realized in the aspects of energy sources and environment.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation to produce clean energy, and in particular relates to a method for improving the butanol-producing activity of anaerobic bacteria. Background technique [0002] The year-by-year exploitation and utilization of fossil energy has reduced the storage of fossil energy worldwide, and the continuous increase in oil prices has resulted in an extremely severe energy situation and intensified environmental problems. With the rapid growth of population and the rapid development of social economy, the world's energy demand is increasing day by day. Under the dual pressure of energy depletion and environmental degradation, countries around the world are actively developing renewable and green energy to replace traditional fossil energy. Bioenergy is an important clean energy that can replace fossil fuels. Butanol is a new type of biofuel with great potential. Compared with the current popular...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/16C12R1/145
CPCC12P7/16Y02E50/10
Inventor 曹广丽圣亚春武继文赵磊
Owner HARBIN INST OF TECH
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