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Method of using glutamine to inhibit Pb absorption of Brassica rapa subsp. Chinensis and increase ability to resist Pb contamination

A technology of glutamine and glutamine mother, applied in the field of agricultural biology, can solve problems such as metabolic process disorder, plant death, rare heavy metals, etc., and achieve the effects of reducing accumulation and reducing pollution

Active Publication Date: 2017-11-03
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the heavy metal content in the plant accumulates to a certain extent, it will cause the metabolic process disorder in the plant, affect the normal growth and development of the plant, and cause the death of the plant in severe cases
At present, there are extensive studies on the stress effects of heavy metals including Pb on plants and their mechanisms at home and abroad, but there are relatively few research reports and application technologies on reducing the damage of heavy metals to plants.

Method used

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  • Method of using glutamine to inhibit Pb absorption of Brassica rapa subsp. Chinensis and increase ability to resist Pb contamination
  • Method of using glutamine to inhibit Pb absorption of Brassica rapa subsp. Chinensis and increase ability to resist Pb contamination
  • Method of using glutamine to inhibit Pb absorption of Brassica rapa subsp. Chinensis and increase ability to resist Pb contamination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Inhibition of different concentrations of glutamine on Pb pollution in Chinese cabbage

[0015] Mix nutrient soil, perlite and vermiculite with water in a ratio of 4:1:1. The basic characteristics of the mixed nutrient soil are as follows: pH 6.3, total nitrogen content 1.38 g / kg, total phosphorus content 0.12%, and total potassium content 2.1 %, the total organic matter content is 21.2 g / kg, the available nitrogen content is 0.11 g / kg, the available phosphorus content is 11.2 mg / kg, and the lead content is 13 mg / kg. Get 60 plastic seedling pots, add 5 Kg of the above-mentioned mixed nutrient soil to each pot, and add Pb(NO 3 ) 2 Make the Pb content in each pot of nutrient soil be 400 mg / k. The above seedling pots were equally divided into 4 groups (15 pots in each group).

[0016] The pakchoi (Silkworm) seeds (purchased from Zhejiang Academy of Agricultural Sciences) were sown in seedling pots with a row spacing of 10 × 10 cm, and 50 plants were planted i...

Embodiment 2

[0021] Example 2: Effect of glutamine treatment on the Pb content of Chinese cabbage roots and aerial parts grown in soil with different Pb pollution concentrations

[0022] Take 60 plastic seedling pots, add 5 Kg of the above-mentioned mixed nutrient soil to each pot, and divide the above-mentioned seedling pots into 3 groups on average (20 pots in each group). Add Pb(NO 3 ) 2 The Pb content in the nutrient soil of the first group of seedling pots was 200 mg / k, the Pb content of the nutrient soil of the second group of seedling pots was 400 mg / k, and the Pb content of the third group of nutrient soil in the seedling pots was 600 mg / k. According to the method described in Example 1, sow pak choy in each of the above pots. At the same time, culture was carried out according to the method described in Example 1.

[0023] 10 days after sowing, divide each group of seedling pots into 2 groups on average, spray 200 mL of 10 mmol / L glutamine evenly on the first group of pak choy ...

Embodiment 3

[0028]Embodiment 3: The influence of glutamine treatment on the growth of Chinese cabbage in different Pb polluted soils

[0029] Take 40 plastic seedling pots, add 5 Kg of the above-mentioned mixed nutrient soil to each pot, and divide the above-mentioned seedling pots into 4 groups on average (10 pots in each group). Pb(NO 3 ) 2 The Pb content in the nutrient soil of the first group of seedling pots was 200 mg / k, the Pb content of the nutrient soil of the second group of seedling pots was 400 mg / k, and the Pb content of the third group of nutrient soil in the seedling pots was 600 mg / k. Add the same amount of deionized water (0 mg / kg Pb) to the fourth group of seedling pots. According to the method described in Example 1, sow pak choy in each of the above pots. At the same time, culture was carried out according to the method described in Example 1.

[0030] Ten days after sowing, the first to third groups of seedling pots were divided into two groups on average, and 10 ...

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Abstract

The invention discloses application and method of glutamine to inhibit heavy metal Pb absorption of plants to relieve vegetable heavy metal contamination and reduce vegetable damage by Pb and growth inhibition by Pb. Soil Pb absorption by plants is inhibited via glutamine treatment herein; by applying 10 mmol / L of glutamine to Brassica rapa subsp. Chinensis and other vegetables grown in soil with different Pb contamination levels, it is possible to effectively inhibit soil Pb absorption by Brassica rapa subsp. Chinensis, reduce Pb accumulation in roots and stem leaves of Brassica rapa subsp. Chinensis, and relieve Pb contamination of Brassica rapa subsp. Chinensis.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and specifically relates to an application and a method for using glutamine treatment to inhibit the absorption of heavy metal Pb by pakchoi and improving the anti-Pb pollution ability of pakchoi. Background technique [0002] The people regard food as the first priority, and food safety is the first priority. Food safety is a major issue related to the national economy and people's livelihood. Agricultural products can not only be eaten directly, but also the basic raw materials for food processing. Their safety has a decisive impact on the safety of ordinary people's vegetable baskets and rice bags. In recent years, the safety of agricultural products caused by soil and other environmental heavy metal pollution has become increasingly serious. Environmental pollution safety incidents of agricultural products with excessive heavy metal content including lead (Pb) have occurred frequentl...

Claims

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Application Information

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IPC IPC(8): A01N37/46A01P21/00
CPCA01N37/46
Inventor 徐茂军董菊芳郑耀奇
Owner HANGZHOU NORMAL UNIVERSITY
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