A kind of multi-layer hydrogel with hollow tube structure and its preparation method and application

A hydrogel and empty tube technology, applied in the field of material science and engineering, to achieve strong bionic effect, improve nutrient transport efficiency, and good scalability

Active Publication Date: 2020-04-28
上普博源(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current hydrogel step-by-step coating methods, after forming a layer, need to replenish the cross-linking agent or the components required for the cross-linking reaction to form the next layer, and many polyelectrolyte-based cross-linking processes are in strong acid Or strong alkaline conditions are not suitable for the encapsulation of living cells

Method used

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  • A kind of multi-layer hydrogel with hollow tube structure and its preparation method and application
  • A kind of multi-layer hydrogel with hollow tube structure and its preparation method and application
  • A kind of multi-layer hydrogel with hollow tube structure and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1, Preparation of two-stage bifurcated, multilayer hydrogel hollow tube structure

[0072] according to figure 1 The flow chart shown was prepared.

[0073] (1) Preparation of bifurcated gel core

[0074] A two-stage bifurcated mold made of PLA material was manufactured with an FDM printer, and an aqueous gelatin solution containing calcium chloride (0.02g / ml) was poured. The solution has the characteristic of dissolving the low-temperature gel at high temperature, and the process is reversible), and a bifurcated gel core with a diameter of 3 mm is obtained.

[0075] (2) Preparation of the first layer of calcium alginate gel

[0076] At room temperature, soak the prepared bifurcated gel core in sodium alginate aqueous solution (concentration: 0.02g / ml), wait for 30s, due to the diffusion of calcium ions (cross-linking agent) into the sodium alginate solution And cause a cross-linking reaction, the surface of the gel core after taking out is wrapped with a l...

Embodiment 2

[0087] Example 2, Preparation of two-stage bifurcated, cell-containing multilayer hydrogel hollow tube structure

[0088] The preparation method is basically the same as in Example 1, except that:

[0089] 1) In the preparation step of the first layer of calcium alginate gel, soak the gel core in sodium alginate aqueous solution containing endothelial cells (the cell concentration is 2.5×106 individual / ml), to obtain the first layer of endothelial cell gel layer;

[0090] 2) In the preparation step of the second layer of calcium alginate gel, the gel core was soaked in an aqueous solution of sodium alginate containing fibroblasts (the cell concentration was 2.5×10 6 individual / ml), to obtain the second layer of fibroblast gel layer;

[0091] 3) In the preparation step of the third layer of calcium alginate gel, the gel core was soaked in an aqueous solution of sodium alginate containing endothelial cells (the cell concentration was 2.5×10 6 individual / ml), to obtain the thir...

Embodiment 3

[0098] Example 3, construction of three-dimensional thick tissue containing vascular network

[0099] according to Figure 8 The schematic diagram shown is constructed, and the icons: 10 represent the prefabricated three-dimensional bifurcated hollow multilayer vascular network, 11 represent the three-dimensional thick tissue manufacturing process, 12 represent the blood or culture solution perfusion direction, 13 represent the tissue structure manufactured by step 11, 14 represents a multi-layered vessel wall structure, and 15 represents a hollow vessel channel.

[0100] Place the prefabricated vascular network prepared in Example 2 in a matching tissue-forming cavity, exposing the inlet and outlet nozzles of the perfusion culture; around the vascular network, form a structure containing specific tissue cells into the tissue-forming cavity, which is manufactured by step 11 Surrounding the three-dimensional thick tissue structure of the vascular network, the prefabricated vas...

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Abstract

The invention discloses multilayer hydrogel of a hollow tube structure and a preparation method and application of the multilayer hydrogel. The preparation method comprises the following steps: (1) preparing a temperature sensitive gel core containing water-soluble divalent cation salt; (2) soaking the temperature sensitive gel core in a sodium alginate solution, and forming a layer of alginate gel on the surface of the temperature sensitive gel core by virtue of cross-linking reaction; (3) repeating the step (2) for the temperature sensitive gel core treated in the step (2), i.e. forming multiple layers of alginate gel on the surface of the temperature sensitive gel core, and removing the temperature sensitive gel core, thus obtaining the multilayer hydrogel. The method is simple in operation, and rapid in manufacturing, and a hydrogel structure of more than 10 layers can be formed in several minutes; a formed hollow tube is flexible in geometric shape, can be branched, and can be varied in diameter and shapes mainly depending on the geometric shape of the temperature sensitive gel core; the production process is mild in treatment environment and nondestructive to cells, and the multilayer gel structure can support the loading of the cell, macromolecules and small molecules, and the application prospect is wide.

Description

technical field [0001] The invention relates to a multilayer hydrogel with a hollow tube structure, a preparation method and application thereof, and belongs to the field of material science and engineering technology. Background technique [0002] Hydrogel is a kind of gel with water as the dispersion medium. Among them, biomedical hydrogel is a hot spot in recent years, and is widely used as a carrier for drugs, nucleic acids, bacteria, cells, etc. In particular, many biocompatible hydrogels can mimic the components and structures of the extracellular matrix, and are used to attach or wrap cells for the construction of biomimetic tissues and organs. Based on the basic needs of cell growth for a water-soluble environment, hydrogel materials are considered to be the most important carrier materials for encapsulating cells. [0003] The damage, loss and dysfunction of tissues and organs have always been a major problem affecting human health. The development of biomaterials ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/52A61L27/20A61L27/38A61L27/50A61L27/54G01N33/15G16H50/50
CPCA61L27/20A61L27/3804A61L27/3808A61L27/3826A61L27/50A61L27/52A61L27/54A61L2300/258A61L2300/416A61L2430/02A61L2430/06A61L2430/20A61L2430/26A61L2430/28G01N33/15C08L5/04
Inventor 欧阳礼亮孙伟赵雨
Owner 上普博源(北京)生物科技有限公司
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