Ion Repulsion Method for Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometer

Abstract

An ion repulsion method of a matrix-assisted laser desorption / ionization time-of-flight mass spectrometer relates to the time-of-flight mass spectrometer, enables the matrix ions to be deflected out by an electric field before reaching a detector to thereby enable the detector only to receive the sample ions, enables the service life of the detector to be prolonged and the signal to noise ratio of an instrument to be increased simultaneously. The method comprises the steps that a deflection electrode group is set in a field-free area of a time-of-flight mass analyzer; a laser pulse signal is detected, synchronized, and broadened; the broadened laser pulse signal is amplified, is converted into a synchronous high-voltage pulse, and is applied on the deflection electrode group; before reaching the detector, the matrix micromolecules deflect by the action of a pulsed electric field and cannot reach the detector, while the sample macromolecules pass the deflection electrodes after the pulse action time, are not influenced by the electric field and are detected normally.

Description

technical field [0001] The invention relates to a time-of-flight mass spectrometer, in particular to an ion repulsion method for a matrix-assisted laser desorption ionization time-of-flight mass spectrometer. Background technique [0002] Matrix Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometer (Matrix Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometer, MALDI-TOFMS) is a new type of soft ionization biological mass spectrometer developed in recent years. With the characteristics of high sensitivity, high accuracy and high resolution, it has been widely used in genomics and proteome research. [0003] Matrix-assisted laser desorption ionization time-of-flight mass spectrometer is mainly composed of two parts: matrix-assisted laser desorption ionization source (MALDI) and time-of-flight mass analyzer (TOF). Its basic principle: the sample is dispersed in the matrix molecules and forms a co-crystal. When the crystal is irradiated with las...

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Problems solved by technology

But the existence of the matrix also brings some problems at the same time: the bulk analysis will also occur during the ionization process of the matrix molecules absorbing energy from the laser pulse, and reach the detector to give ion signals, resulting in 1) the detector receives a large amount of useless Matrix ions shorten the life of the detector; 2) matrix ions will interfere with the detection of sample molecules at the same time, reducing the signal-to-noise ratio of the instrument

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