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High-yield and high-selenium cordyceps militaris production method

A production method and a technology for Cordyceps militaris are applied in the directions of botanical equipment and methods, applications, organic fertilizers, etc., can solve problems such as toxicity and difficult absorption by the human body, and achieve the effects of promoting growth and development, low cost, and easy large-scale promotion and production.

Active Publication Date: 2017-11-10
ANKANG AGRI SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If supplemented with inorganic selenium, it is difficult for the human body to absorb, and it has certain toxicity
However, relying solely on selenium in natural foods cannot meet the needs of Chinese residents.

Method used

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  • High-yield and high-selenium cordyceps militaris production method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A kind of production method of present embodiment high yield, high selenium Cordyceps militaris, concrete process is as follows:

[0037] S1: Activation of Cordyceps militaris

[0038] Prepare PDA solid medium according to the conventional method, and its medium formula is as follows: potato 200g, glucose 10g, sucrose 5.0g, peptone 5.0g, potassium dihydrogen phosphate 1.0g, magnesium sulfate 0.5g, agar 12g, water 1000mL. The preserved Cordyceps militaris strains were inoculated on the above-mentioned PDA solid medium, activated and cultured for 7 days, and when the mycelia covered 3 / 4 of the test tube, cultured under light for 12 hours to complete the strain activation of the Cordyceps militaris strains.

[0039] S2: Liquid strain preparation

[0040] The strains activated through the above steps are selected from the test tube strains that have undergone color change, and the strains are inoculated in liquid medium for fermentation and culture. The culture time is 5-7...

Embodiment 2

[0051] The production method of a kind of high-yield, high-selenium Cordyceps militaris of the present embodiment, concrete process is identical with embodiment 1, difference only is, in S3, sodium selenite is added according to the amount of 150mg per liter of basic culture solution, methionine Acid was added in an amount of 450 mg per liter of basal culture solution.

Embodiment 3

[0053] The production method of a kind of high-yield, high-selenium Cordyceps militaris of the present embodiment, concrete process is identical with embodiment 1, and difference is only, in S3, sodium selenite is added according to the amount of 200mg per liter of basic culture solution, methionine Acid was added in an amount of 800 mg per liter of basal culture solution.

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Abstract

The embodiment of the invention discloses a high-yield and high-selenium cordyceps militaris production method, and belongs to the technical field of edible fungus cultivation. The method comprises the steps of cordyceps militaris strain activation, liquid strain preparation, culture medium preparation, inoculation culture, cultivation management achieved in a full-light cultivation and oxygen enrichment cultivation mode and the like. According to the method, sodium selenite serves as a selenium source, so that the cost is low; methionine is added so that the selenium element can be easily absorbed by mycelia; the selenium in a substrate is solidified into cordyceps militaris through metabolism of substances in cordyceps militaris mycelium cells, and the selenium content of cordyceps militaris stromata is greatly increased.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, in particular to a production method of high-yield and high-selenium Cordyceps militaris. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, belongs to Eumycota, Ascomycotina, Pyrenomycetes, Clavicipitales, Clavicipitaceae, Cordyceps (Cordyceps), which belong to the same species as Cordyceps sinensis, is the model species of the genus Cordyceps. A large number of studies have shown that the pharmacological effects of Cordyceps militaris are very similar to those of Cordyceps sinensis. Immunity etc. [0003] Selenium is one of the essential trace elements for the human body, and it is also internationally recognized as "the king of anti-cancer elements" and "human immune activator". Cordyceps militaris has extremely high nutritional value and health care value, and selenium is particularly important to human health. Ther...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
CPCA01G18/00C05B7/00C05C3/00C05C11/00C05D5/00C05D9/02C05F11/00
Inventor 张园园王勇张晶叶岚韩宝康杨柱胡波江新华
Owner ANKANG AGRI SCI RES INST