Composite nerve conduit and preparation method thereof

A nerve conduit and catheter technology, applied in the field of biomedical tissue engineering, can solve the problems of unfavorable surface morphology for nerve cell adhesion and growth, failure to provide biochemical clues for nerve cell regeneration and growth neurotrophic factors, etc., to achieve excellent mechanical properties, preparation The method is simple and the effect of uniform wall thickness

Active Publication Date: 2017-11-14
北京智枢生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned patents can only bridge the broken ends of nerve defects, but cannot provide biochemical clues such as neurotrophic factors required for nerve cell regeneration and growth, and its surface morphology is not conducive to the adhesion and growth of nerve cells

Method used

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  • Composite nerve conduit and preparation method thereof
  • Composite nerve conduit and preparation method thereof
  • Composite nerve conduit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, preparation composite nerve guide

[0043] Prepare the composite nerve guide as follows:

[0044] (1) Prepare the outer catheter:

[0045] 1) Under room temperature conditions, a certain amount of chitosan was dissolved in 2wt% acetic acid to prepare a solution with a mass fraction of 6wt%. Dissolve a certain amount of polyvinyl alcohol in acetic acid of the same concentration, heat in a water bath at 95°C for 1 hour, and prepare a solution with a mass fraction of 12 wt%. Mix the above two solutions in different proportions, stir evenly, and centrifuge to remove air bubbles before use. The mass ratio of chitosan and polyvinyl alcohol in the mixture is 1:2.

[0046] 2) Using catheter forming equipment, dip the chitosan-polyvinyl alcohol mixture on a mold with a length of 5cm and a diameter of 4mm, fix the stainless steel mold on the brushless motor, and control the mold at a speed of 700rpm through the motor controller Horizontal rotation. After the so...

Embodiment 2

[0054] Embodiment 2, performance test

[0055] 1. Mechanical properties

[0056] The mechanical properties of the outer catheter prepared in step (1) in Example 1 were tested by a universal mechanical tensile testing machine, the calibration distance was 30 mm, and the tensile rate was 5 mm / min. The results show( image 3 ), the outer conduit has superior mechanical properties, with a maximum load of 19N.

[0057] 2. Biological activity

[0058] Taking E8 chick embryo dorsal root ganglion (DRG) as the test object, the biological activity of the composite nerve conduit prepared in Example 1 was verified by the chick embryo dorsal root ganglion (DRG) culture method. The specific operation is as follows:

[0059] Chicken dorsal root ganglia extracted from 8-day-old chicken embryos were placed in the composite nerve conduit, and the composite nerve was co-cultured with the conduit for 48 hours, then the composite nerve conduit was cut, and the dorsal root ganglion was observed...

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Abstract

The invention discloses a composite nerve conduit and a preparation method thereof. The method comprises the following steps of (1) preparing an outer-layer conduit; (2) preparing an inner-layer nanofiber membrane; (3) under the action of an initiator, reacting the inner-layer nanofiber membrane prepared through the step (2) with a polypeptide growth factor, and thus obtaining an inner-layer nanofiber membrane loaded with the polypeptide growth factor; (4) installing the inner-layer nanofiber membrane loaded with the polypeptide growth factor prepared through the step (3) in the outer-layer conduit in the step (1) in a casing way, and thus obtaining the composite nerve conduit. In the composite nerve conduit, the outer-layer conduit has an excellent mechanical property, and can provide a stable microenvironment for growth and proliferation of internal nerve cells. The inner-layer nanofiber membrane provides a physical support for the attachment of the nerve cells, and the polypeptide growth factor loading on the inner-layer nanofiber membrane can simulate a development and growth environment of the nerve cells, so that a biochemistry clue is provided for neurite growth.

Description

technical field [0001] The invention belongs to the field of biomedical tissue engineering, and in particular relates to a composite nerve guide and a preparation method thereof. Background technique [0002] After nerve injury, the peripheral nervous system can achieve self-repair to a certain extent. Schwann cells reactively proliferate to form cell cords, and at the same time, axon shoots grow, and extend into the cell cords after passing through the injured area. Axons in the cell cords The shoot buds continue to grow towards the target cells and eventually form synaptic connections with the target cells. However, if the injury distance is too long, or due to the obstruction of the surrounding connective tissue, the nerve stumps cannot be directly anastomosed, and proliferative neuroma-like tissue will be formed at the nerve injury site, which will affect the function of the limbs. [0003] Central nervous system injury will cause more serious consequences. For example,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/50
Inventor 李晓光王柏曦杨朝阳高钰丹
Owner 北京智枢生物科技有限公司
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