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A kind of lysinic bacillus that degrades the sour and odorous matter of dairy cow manure and its application

A technology of Bacillus lysine, dairy cow feces, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of a large number of manpower and equipment investment and consumption, improve biomass and catalytic performance, strong growth adaptability, The effect of high resistance to organic acids

Active Publication Date: 2020-03-17
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the traditional centralized treatment method is used for manure treatment, it is necessary to wash the manure with water, and then process it through solid-liquid separation, anaerobic fermentation, composting, etc. This method requires a lot of manpower, water and electricity, equipment investment and consumption

Method used

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  • A kind of lysinic bacillus that degrades the sour and odorous matter of dairy cow manure and its application
  • A kind of lysinic bacillus that degrades the sour and odorous matter of dairy cow manure and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Enrichment culture and separation and purification of Bacillus lysine LF-N1

[0025] Take 0.2g of cow feces accumulation and inoculate it in 100mL liquid screening medium. The medium formula is: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, 5g / L dry cow manure, acetic acid , Propionic acid, n-butyric acid, isobutyric acid, n-valeric acid, isovaleric acid are each 1g / L, as well as 0.1g / L indole and 0.1g / L skatole, adjust the pH to 7.0; culture 37℃, 200r·min -1 Incubate on a shaker for 48h. Then take 100 μL of the bacterial solution from the above-mentioned culture solution, inoculate it in a new liquid screening medium (the medium formula is the same as above), and repeat the above-mentioned culture operation 2 to 3 times. Then take 100 μL of bacterial solution from the final culture solution and inoculate it in an acclimation medium for acclimation culture. The formula of the acclimation medium is: tryptone 10g / L, yeast extract 5g / L, sodium chloride...

Embodiment 2

[0026] Example 2 SEM observation, identification and species analysis of strains

[0027] The isolated and purified monoclonal colony Bacillus lysine LF-N1 was inoculated into 50mL liquid LB medium. The medium formula is: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, take 5μL The liquid sample is used to prepare a scanning electron microscope sample. In the imaging photo with a magnification of 10,000x, the morphology of Bacillus lysine LF-N1 is as figure 1 Rod shape shown.

[0028] Take 1.5ml of fresh Bacillus lysine LF-N1 culture solution, centrifuge 12,000g for 1min, remove the supernatant, and use the solution provided in the genomic DNA extraction kit (purchased from Axygen) to the cell pellet Resuspend, extract genomic DNA according to the operating instructions of the kit, and finally dissolve the DNA pellet in 100μL TE buffer (10mM Tris, 1mM EDTA, pH8.0), and dilute the genomic DNA to 20mg / L, and take 1μL Used as a template for 16S rDNA amplification. The pri...

Embodiment 3

[0030] Example 3 Analysis of the degradation effect of organic acid odor by Bacillus lysine LF-N1

[0031] A single colony of Lysine Bacillus LF-N1 on the LB plate was inoculated into 50mL liquid screening medium. The medium formula was: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, 5g / L dry cow manure, adjust the pH to 7.0, add 1g / L acetic acid, propionic acid, n-butyric acid, isobutyric acid, n-valeric acid, isovaleric acid, and 0.1g / L indium after the medium is sterilized Indole and skatole. Incubate on a shaker at 37°C and 200r·min-1 for 24 to 48 hours, and then inoculate all the culture solution into 1000ml liquid screening medium. The medium formula is: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, 5g / L dry cow manure, in addition to 0.1g / L indole and skatole, and 1g / L or 2g / L acetic acid, propionic acid, n-butyric acid, isobutyric acid, n-valeric acid, isoamyl Acid, adjust the pH to 7.0; 37°C, 200rpm, shake culture for 24~192h, regularly sample...

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Abstract

The invention discloses a lysine bacillus for degrading sour and odorous substances in cow feces and an application thereof. The Bacillus lysinica LF-N1 provided by the present invention has been preserved in the China Center for Type Culture Collection (CCTCC), and its preservation number is CCTCC No: M 2017289. Lysinibacillus LF‑N1 can effectively degrade the short-chain sour and smelly fatty acid compounds contained in biomass substrates such as wood chips, and is suitable for the production of high-efficiency microbial inoculum products in the ecological breeding mode of dairy cow breeding beds. The Bacillus lysinica LF-N1 in the present invention can also efficiently degrade indole and skatole, two types of organic pollutants in the livestock and poultry breeding industry, so it has a good application prospect in ecological breeding and environmental management, etc. .

Description

Technical field [0001] The invention belongs to the technical field of environmental microbial application, and specifically relates to a lysine bacillus degrading the acid odor of cow feces and its application. Background technique [0002] my country’s livestock and poultry breeding industry is gradually becoming large-scale and intensive, and its breeding mode and breeding technology have been greatly improved. However, this large-scale breeding method promotes the development of my country’s agricultural economy and is also due to the high breeding density. Factors such as restrictions on land use have brought about various environmental pollution problems, causing the largest source of agricultural non-point source pollution to become pollution from the breeding industry, and urging agricultural pollution emissions to gradually exceed industrial non-point source emissions in recent years. [0003] The amount of manure treatment in the dairy farming process is particularly large...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F11/02C02F103/20C12R1/07
CPCC02F11/02C02F2103/20C12N1/20C12N1/205C12R2001/07
Inventor 孙俊松赵利超余从田吴海英史吉平姜标
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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