Cell composition and preparation method and application thereof

A composition and cell technology, applied in the directions of drug combinations, pharmaceutical formulations, and medical preparations containing active ingredients, can solve the problems of inability to cure Crohn's disease, imbalance of the immune system, and inability to effectively prevent the disease. Disease progression, maintenance of biological activity, remarkable effect of treatment

Inactive Publication Date: 2017-11-17
广东颜值科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of Crohn's disease mainly uses glucocorticoids and immunosuppressants as therapeutic drugs. This method can only relieve the disease, but cannot effectively prevent the development of the disease, let alone cure Crohn's disease; and for a long time The use of glucocorticoids can induce complications such as osteoporosis, high blood pressure, hyperglycemia, and central obesity. At the same time, long-term use of immunosuppressants can also cause imbalances in the body's immune system

Method used

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  • Cell composition and preparation method and application thereof
  • Cell composition and preparation method and application thereof
  • Cell composition and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Isolation and extraction of intestinal stem cells, reference: Ye Zhangqun, Liu Guanlin, Chen Zhiqiang. Construction of mouse intestinal stem cell population. "Journal of Huazhong University of Science and Technology (Medical Edition)" [J], 2006.

[0029] Take intestinal stem cells from passage P2 to passage P5, suck out the medium, add 0.25% trypsin for routine digestion, and then make the cell density 1×10 5 cells / mL cell suspension, then add 5 μL anti-Lgr5 monoclonal antibody to 500 μL cell suspension, then incubate at room temperature in the dark for 20 min, and set up a blank isotype control at the same time; after incubation, centrifuge at 1500 r / min for 5 min, discard The supernatant was washed twice with PBS containing 10% FBS, resuspended with 500 μL of PBS, and tested on the machine.

[0030] The surface antigen detection results of intestinal stem cells are shown in Table 1 below:

[0031] Table 1

[0032]

Lrg5 +

first detection

...

Embodiment 2

[0042] 1, randomly select the mice of 90 SPF grades of similar body weight, divide mice into 6 groups, every group of 15 (male and female); then, each mouse is carried out trinitrobenzenesulfonic acid (2,4, 6-Trinitrobenzenesulfonic Acid, TNBS) enema to manufacture the mouse CD model; then, each model mouse was injected intravenously along the tail according to Table 4, injected once a week, and injected continuously for 4 times, and after 1 month of such conventional feeding All the mice were sacrificed by dislocation, and all indexes were detected.

[0043] References for Crohn's mouse modeling: Liu Shun, Sun Keming, Yang Houlai. Study on the therapeutic effect and mechanism of rapamycin on Crohn's disease mouse model. "Chinese Journal of Endemic Diseases" [J]. 2016 .

[0044] Table 4 Preparations for Injection

[0045] group

injection

Blank control group

normal saline

Control group 1

Infliximab

Control group 2

immunosuppressa...

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PUM

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Abstract

The invention belongs to the field of pharmaceutical preparation and particularly relates to a cell composition and a preparation method and application thereof. The cell composition provided by the invention comprises regulatory T cells, intestinal stem cells and IL-33. The regulatory T cells and the intestinal stem cells serve as active cells, an immune system can further be regulated to inhibit excessive autoimmunity and prevent a disease from deteriorating while the intestinal inflammation of a patient is cured, and the cell composition is widely suitable for the patient with the Crohn disease. Cell factors such as the IL-33 are added into the cell composition, all components conduct the synergistic effect and jointly promote cure of the intestinal inflammation of the patient with the Crohn disease, and the therapeutic effect is remarkable.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and in particular relates to a cell composition and its preparation method and application. Background technique [0002] Crohn's disease is an intestinal inflammatory disease of unknown cause. As the main type of inflammatory bowel disease, it has the characteristics of long course, easy recurrence, invasion to any part of the gastrointestinal tract, and difficult to cure. Crohn's disease is a chronic, recurrent and non-specific transmural inflammation of the digestive tract. The lesions are distributed segmentally and can involve any part of the digestive tract. Among them, the terminal ileum is the most common, and colon and anal lesions are also more common. many. At present, the treatment of Crohn's disease mainly uses glucocorticoids and immunosuppressants as therapeutic drugs. This method can only relieve the disease, but cannot effectively prevent the development of the disease...

Claims

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Application Information

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IPC IPC(8): A61K38/20A61P1/00A61P29/00A61K35/17A61K35/12
Inventor 马颖
Owner 广东颜值科技有限公司
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