Penicillium citrinum fungus for thin film degradation and application thereof

A technology for degrading film and Penicillium citrinum, applied in the field of agricultural biology

Active Publication Date: 2017-11-17
INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of biodegradable polyethylene material, the present invention provides screening, identification and application of a strain of degradable polyethylene powder Penicillium citrinum PT1

Method used

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  • Penicillium citrinum fungus for thin film degradation and application thereof
  • Penicillium citrinum fungus for thin film degradation and application thereof
  • Penicillium citrinum fungus for thin film degradation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Screening and Identification of Example 1 Penicillium citrnum PT1

[0029] Screening of Penicillium citrnum PT1: Sprinkle the polyethylene powder exposed in the natural environment for 7 days at 5% (W / V) evenly on the agar-based inorganic salt medium plate, culture at 30°C for 3-7 days, and pick the polyethylene powder. For the strains that can grow on the powder, streak on a solid medium to obtain a single colony (LB medium for bacteria, PDA medium for fungi, Gaoshi No. 1 medium for actinomycetes), and store at 4°C for future use. Inoculate the above-mentioned isolated strains into a liquid inorganic salt medium containing polyethylene powder and polyester biodegradable mulch film with a size of 15cm×2cm, culture on a shaker at 150r / min at 30°C, and observe the degradation of polyethylene powder and mulch film. Screening for strains capable of rapidly degrading polyethylene materials.

[0030] Identification of Penicillium citrnum PT1: extract the total DNA of the fun...

Embodiment 2

[0031] Example 2 Detection of Penicillium citrnum PT1's ability to degrade polyethylene powder

[0032] method:

[0033] Sterilization of polyethylene powder: Place polyethylene powders of different molecular weights (2000, 400000) in dry sterile stainless steel pans, spread them into thin layers, and irradiate them under a ultra-clean table ultraviolet lamp for 4 hours.

[0034] Penicillium citrnum PT1 degraded polyethylene powder test method is: add 100ml basic inorganic salt culture medium in 250ml Erlenmeyer flask, sterilize with high temperature and damp heat for standby, add 2 kinds of molecular weight (2000, 400000) polyethylene powder (with Sterilization), the addition amount is 5g / L, the inoculation loop is inserted into the Penicillium citrinum PT1 fungus, 150r / min, 30°C shaker culture, observe the change of polyethylene powder, and collect the gas and degradation effect images in the bottle every 7d ( figure 2 ), gas chromatography to determine gas composition and...

Embodiment 3

[0039] Example 3 Detection test of Penicillium citrnum PT1 degrading biodegradable plastic film

[0040] method:

[0041] Sterilization method of plastic film: soak in sterile 3% KCl solution for more than 30 minutes, rinse with sterile water for 3 times, soak in alcohol for 15 minutes (stirring is required during the soaking process, which is conducive to full disinfection), rinse with sterile water for 3 times, absorb with sterile filter paper After drying the surface moisture, dry it with ultra-clean typhoon and irradiate with ultraviolet light for half an hour.

[0042] Under sterile conditions, inoculate the Penicillium citrnum PT1 strain into the liquid basic inorganic salt medium containing 15cm×2cm sterilized polyester biodegradable plastic film (black and white), 150r / min, 30℃ Cultivate on a shaking table, observe the changes of the plastic film, and set up 3 parallel and blank controls in the experiment. The formula of the basic inorganic salt medium is as described ...

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Abstract

The invention discloses a penicillium citrinum fungus for thin film degradation and an application thereof. The classification and name of the enicillium citrinum are (Penicillium citrinum) PT1, the enicillium citrinum is preserved in the Guangdong General Microbiological Culture Collection Center (GDMCC), and the preservation number is GDMCC No:60130. Polyethylene powder of different molecular masses can be degraded through the PT1 fungi, the PT1 fungi can grow with a biodegradable mulching film and erode a common polyethylene mulching film, can grow with polyethylene as an unique carbon source and rapidly degrade the polyethylene, and meanwhile has the degradation effect on the biodegradable mulching film.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology, and relates to a Penicillium citrinum PT1 fungus capable of degrading films and its application, in particular to a Penicillium citrinum PT1 fungus capable of degrading polyethylene films and / or polyester films and its application. Background technique [0002] Polyethylene is widely used in daily life, chemical industry, construction and other industrial fields due to its light weight, corrosion resistance, wear resistance and good insulation properties. This artificially synthesized polymer compound with stable molecular structure biodegrades in nature Very slow. With the popularity of polyethylene plastics, polyethylene plastics and relatively low cost are often used as the main production raw materials to prepare mulch films. Since the use of mulch in my country in the 1970s, polyethylene mulch is one of the types of agricultural mulch widely used in agriculture. Polyethylene p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A62D3/02C12R1/80A62D101/20A62D101/28
CPCA62D3/02A62D2101/20A62D2101/28C12N1/14C12N1/145C12R2001/80
Inventor 李夏顾文杰杨少海吴杭涛徐培智卢钰升解开治孙丽丽
Owner INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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