Ferriporphyrin organic frame material peroxidase mimic, and preparation method and application thereof
An organic framework and material simulation technology, which is applied in the direction of chemical reaction of materials for analysis, material analysis, chemiluminescence/bioluminescence, etc., can solve the problems of restriction enzyme application, preparation and purification, expensive storage, instability, etc., to achieve Easy post-treatment, good peroxidase activity, and low detection limit
Inactive Publication Date: 2017-11-17
UNIV OF JINAN
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The invention discloses a ferriporphyrin organic frame material peroxidase mimic. The erriporphyrin organic frame material is prepared from 1,6,7,12-tetrachloro-3,4,9,10-tetracarboxylic acid dianhydride and 5,10,15,20-tetra(4-aminophenyl) porphyrin iron through a solvothermal technology. The ferriporphyrin organic frame material has excellent peroxidase-like catalysis activity, can rapidly and effectively catalyze 3,3',5,5'-tetramethyl benzidine oxidation for generating a typical blue solution in the presence of hydrogen peroxide, and can be used for detecting low-concentration H2O2, and the absorbance of a catalysis product is linearly related with the concentration, in a concentration range, of the hydrogen peroxide. The linear range of detection H2O2 is 1-200 [mu]M, and the detection limit is 0.14 [mu]M.
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Property | Measurement | Unit |
Michaelis constant | 0.18 | mM |
Michaelis constant | 0.78 | mM |
tensile | MPa | |
Particle size | Pa | |
strength | 10 |
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