Ferriporphyrin organic frame material peroxidase mimic, and preparation method and application thereof

An organic framework and material simulation technology, which is applied in the direction of chemical reaction of materials for analysis, material analysis, chemiluminescence/bioluminescence, etc., can solve the problems of restriction enzyme application, preparation and purification, expensive storage, instability, etc., to achieve Easy post-treatment, good peroxidase activity, and low detection limit

Inactive Publication Date: 2017-11-17
UNIV OF JINAN
2 Cites 18 Cited by

AI-Extracted Technical Summary

Problems solved by technology

However, most natural enzymes are proteins in nature, which have unavoidable shortcomings such as instab...
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Abstract

The invention discloses a ferriporphyrin organic frame material peroxidase mimic. The erriporphyrin organic frame material is prepared from 1,6,7,12-tetrachloro-3,4,9,10-tetracarboxylic acid dianhydride and 5,10,15,20-tetra(4-aminophenyl) porphyrin iron through a solvothermal technology. The ferriporphyrin organic frame material has excellent peroxidase-like catalysis activity, can rapidly and effectively catalyze 3,3',5,5'-tetramethyl benzidine oxidation for generating a typical blue solution in the presence of hydrogen peroxide, and can be used for detecting low-concentration H2O2, and the absorbance of a catalysis product is linearly related with the concentration, in a concentration range, of the hydrogen peroxide. The linear range of detection H2O2 is 1-200 [mu]M, and the detection limit is 0.14 [mu]M.

Application Domain

Chemiluminescene/bioluminescence

Technology Topic

SolventEnvironmental chemistry +10

Image

  • Ferriporphyrin organic frame material peroxidase mimic, and preparation method and application thereof
  • Ferriporphyrin organic frame material peroxidase mimic, and preparation method and application thereof
  • Ferriporphyrin organic frame material peroxidase mimic, and preparation method and application thereof

Examples

  • Experimental program(9)

Example Embodiment

[0041] Example 1:
[0042] Respectively mix 106mg 0.2mmol of 1,6,7,12-tetrachloro-3,4,9,10-tetracarboxylic dianhydride (TAD), 50mg0.1mmol of 5,10,15,20-tetrakis (4-amino Phenyl) iron porphyrin (FeTAPP), 1000mg imidazole and activated The type molecular sieve was added to a 20mL heat-resistant glass tube, and then subjected to three cycles of freeze-pump-thaw degassing treatment, the tube was sealed, and the reaction was heated at 170°C for 3 days. The precipitate was obtained by centrifugal separation and washed with distilled water, 3% NaOH solution, ethanol, and chloroform; the product was vacuum dried at 120°C overnight.

Example Embodiment

[0043] Example 2:
[0044] In the presence of hydrogen peroxide, the catalytic activity of TAP-COF for catalytic oxidation of TMB was studied using the following method: 200μL 0.32mg/mL TAP-COF, 200μL H 2 O 2 (30mM) and 200μL of TMB (0.8mM) were added to HAc-NaAc (0.2M, pH3.8) buffer to maintain a total reaction volume of 2mL. Incubate for another 10 min at room temperature. The solution changed from colorless to blue image 3 , See the absorption peak at 652nm Figure 4.

Example Embodiment

[0045] Example 3:
[0046] Add 200μL 0.32mg/mL TAP-COF and 200μL H to HAc-NaAc solution with pH values ​​of 1.7, 2.6, 3.8, 5, 5.8, 6.6, 7.1, 8 respectively. 2 O 2 (30mM) and 200μL TMB (0.8mM), maintaining a total reaction volume of 2mL. Incubate for another 10 min at room temperature. Measure the absorbance at 652nm wavelength, see Figure 5.
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PUM

PropertyMeasurementUnit
Michaelis constant0.18mM
Michaelis constant0.78mM
tensileMPa
Particle sizePa
strength10

Description & Claims & Application Information

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Classification and recommendation of technical efficacy words

  • Easy to prepare
  • Post-processing is simple

People also interested in

High affinityLow detection limitHigh sensitivityGood stabilityHydrogen peroxideFerricChlorine
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