Ferriporphyrin organic frame material peroxidase mimic, and preparation method and application thereof

An organic framework and material simulation technology, which is applied in the direction of chemical reaction of materials for analysis, material analysis, chemiluminescence/bioluminescence, etc., can solve the problems of restriction enzyme application, preparation and purification, expensive storage, instability, etc., to achieve Easy post-treatment, good peroxidase activity, and low detection limit

Inactive Publication Date: 2017-11-17
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most natural enzymes are proteins in nature, which have unavoidable shortcomings such as instab

Method used

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  • Ferriporphyrin organic frame material peroxidase mimic, and preparation method and application thereof
  • Ferriporphyrin organic frame material peroxidase mimic, and preparation method and application thereof
  • Ferriporphyrin organic frame material peroxidase mimic, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0041] Example 1:

[0042] Respectively mix 106mg 0.2mmol of 1,6,7,12-tetrachloro-3,4,9,10-tetracarboxylic dianhydride (TAD), 50mg0.1mmol of 5,10,15,20-tetrakis (4-amino Phenyl) iron porphyrin (FeTAPP), 1000mg imidazole and activated The type molecular sieve was added to a 20mL heat-resistant glass tube, and then subjected to three cycles of freeze-pump-thaw degassing treatment, the tube was sealed, and the reaction was heated at 170°C for 3 days. The precipitate was obtained by centrifugal separation and washed with distilled water, 3% NaOH solution, ethanol, and chloroform; the product was vacuum dried at 120°C overnight.

Example Embodiment

[0043] Example 2:

[0044] In the presence of hydrogen peroxide, the catalytic activity of TAP-COF for catalytic oxidation of TMB was studied using the following method: 200μL 0.32mg / mL TAP-COF, 200μL H 2 O 2 (30mM) and 200μL of TMB (0.8mM) were added to HAc-NaAc (0.2M, pH3.8) buffer to maintain a total reaction volume of 2mL. Incubate for another 10 min at room temperature. The solution changed from colorless to blue image 3 , See the absorption peak at 652nm Figure 4 .

Example Embodiment

[0045] Example 3:

[0046] Add 200μL 0.32mg / mL TAP-COF and 200μL H to HAc-NaAc solution with pH values ​​of 1.7, 2.6, 3.8, 5, 5.8, 6.6, 7.1, 8 respectively. 2 O 2 (30mM) and 200μL TMB (0.8mM), maintaining a total reaction volume of 2mL. Incubate for another 10 min at room temperature. Measure the absorbance at 652nm wavelength, see Figure 5 .

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Abstract

The invention discloses a ferriporphyrin organic frame material peroxidase mimic. The erriporphyrin organic frame material is prepared from 1,6,7,12-tetrachloro-3,4,9,10-tetracarboxylic acid dianhydride and 5,10,15,20-tetra(4-aminophenyl) porphyrin iron through a solvothermal technology. The ferriporphyrin organic frame material has excellent peroxidase-like catalysis activity, can rapidly and effectively catalyze 3,3',5,5'-tetramethyl benzidine oxidation for generating a typical blue solution in the presence of hydrogen peroxide, and can be used for detecting low-concentration H2O2, and the absorbance of a catalysis product is linearly related with the concentration, in a concentration range, of the hydrogen peroxide. The linear range of detection H2O2 is 1-200 [mu]M, and the detection limit is 0.14 [mu]M.

Description

technical field [0001] The invention relates to an iron porphyrin organic framework material with the characteristic of simulating peroxidase, and belongs to the technical field of functional complexes and bionics. Background technique [0002] Endogenous small molecules are produced by a variety of different biochemical reactions in the human body, which can truly reflect the pathological and physiological processes of tissue cell metabolism in the body, and have become a hotspot in the research of disease markers. Has shown excellent practical value. Therefore, detecting endogenous small molecules and revealing their levels and abnormal expression will be of great significance for non-invasive early warning, screening and diagnosis of diseases. [0003] due to H 2 o 2 It can affect most of the biochemical reactions in the human body and plays an important role in human health. In recent years, H 2 o 2 detection has attracted a lot of attention. Various quantitative ...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N21/3577
Inventor 朱沛华宋非非王玉栋王榆成
Owner UNIV OF JINAN
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