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Naringin extraction method

An extraction method and naringin technology, applied in the field of naringin extraction, can solve the problems of toxicity, low extraction rate, high operating cost, etc., and achieve the effects of reducing the amount of raw materials and enzymes, and reducing production costs.

Active Publication Date: 2017-11-28
陈泓达 +1
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  • Application Information

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Problems solved by technology

The existing extraction methods of naringin mainly include hot water method, alcohol extraction method, alkali extraction and acid precipitation method, supercritical CO 2 Extraction method, ultrasonic-assisted extraction method, enzymatic heating water extraction method (refer to applicant’s prior patent application ZL201510895047.3), in which supercritical CO 2 The equipment and operating costs required by the extraction method are relatively expensive, while the alcohol extraction method and the alkali extraction and acid precipitation method often use toxic methanol, or ethanol, calcium hydroxide and other components that are not conducive to subsequent food processing, and the existing methods of extraction The ratio is not high, basically no more than 7% by naringin weight to material weight (see Hubei Agricultural Science, Volume 50, No. 8, April 2011, the extraction method of naringin and its application research progress)

Method used

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  • Naringin extraction method

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The preparation of embodiment 1 naringin

[0027] (1) Take by weighing 1kg of fresh pomelo peel, add 7kg of water and soak for 6 hours;

[0028] (2) Add 250,000 U (5g) of bromelain, adjust the pH to 7.0, perform enzymatic hydrolysis at 50°C for 30 minutes, heat to 100°C to inactivate the enzyme for 10 minutes, and cool down;

[0029] (3) Add 50000U (5g) of cellulase and 100000U (2.5g) of pectinase, adjust the pH to 4.5, perform enzymatic hydrolysis at 50°C for 1 hour, heat to 100°C to inactivate the enzyme for 10 minutes, and then cool;

[0030] (4) filtering to obtain the naringin extract;

Embodiment 2

[0031] Example 2 Determination of naringin extraction rate

[0032] Chromatographic conditions:

[0033] The mobile phase is methanol-water-glacial acetic acid (35:61:4), the flow rate is 1.0ml / min, the detection wavelength is 283nm, and the column temperature is 25°C. Preparation of standard curve:

[0034] Accurately weigh 20 mg of naringin standard substance, and set the volume to 20 mL with methanol to obtain a 1 mg / mL standard solution. Draw 1 mL, 2 mL, 4 mL, 6 mL, and 9 mL of the standard solution into a 10 mL volumetric flask, and take a sample of 20 μL. Conditions were used to determine the peak area, with the peak area as the ordinate and the concentration as the abscissa, a linear regression was performed. The regression equation is: Y=28.753X+680.2 (r=0.9993).

[0035] Get the naringin extract obtained in Example 1 step (4) and the naringin extract (amylase , pectinase and cellulase enzymatic hydrolysis system) each in three parts, after centrifuging at 3000r / mi...

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Abstract

The invention provides a naringin extraction method. Naringin is extracted from shaddock peel with an enzyme hydrothermal process. Particularly, naringin is extracted from the shaddock peel by using 250 U / g bromelain, 50 U / g cellulase and 100 U / g pectinase assisted with a hot water process in the naringin extraction method.

Description

a technical field [0001] The invention relates to the field of extracting plant active substances, in particular, the invention relates to a method for extracting naringin. Two background technology [0002] Pomelo is the ripe fruit of pomelo, a plant of Rutaceae. It is produced in Fujian, Jiangxi, Guangdong, Guangxi and other southern regions of China. It tastes sweet and nutritious, and is a popular fruit variety. Traditional Chinese medicine believes that pomelo has the effects of clearing away heat and fire, eliminating food accumulation, constipation, antithrombotic, anti-inflammatory, and lowering blood pressure. It is one of the most therapeutically beneficial fruits. [0003] The active ingredient of pomelo is mainly naringin, which is a flavonoid compound. There is a strong ultraviolet absorption peak at 282nm. The existing extraction methods of naringin mainly include hot water method, alcohol extraction method, alkali extraction and acid precipitation method, su...

Claims

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Application Information

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IPC IPC(8): A23L33/105
CPCA23V2002/00A23V2200/30
Inventor 陈泓达李齐
Owner 陈泓达
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