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Use of functional small peptides extracted from deep-sea fishes

A deep-sea fish and functional technology, applied in the field of small peptide preparation, can solve the problems of loss of activity and low utilization rate, and achieve significant creative effects

Inactive Publication Date: 2017-11-28
兰溪市沉默生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen can be used to repair skin scars, anti-aging, blemishes and whitening, etc. It is a holy product for beauty, but the utilization rate of collagen supplementation through eating is extremely low, and most of it will be decomposed by the body and lose its activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The use of functional small peptides extracted from deep-sea fish, as a protective agent or absorption-promoting agent for collagen, for cosmetics or health products or food or feed or medicine.

[0020] The use of functional small peptides extracted from deep-sea fish includes step-by-step enzymatic hydrolysis, isoelectric point precipitation, secondary ultrafiltration and nanofiltration, and freeze-drying. The step-by-step enzymatic hydrolysis step is to add pure water homogenate to deep-sea fish surimi, then add Porphyra zebra protease and active short peptide for enzymatic hydrolysis, adjust the pH, and then add flavor protease for enzymatic hydrolysis to obtain an enzymatic hydrolysis solution. Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence, and many different active peptides can be released by hydrolyzing marine products with marine proteases. First use Porphyra zebra pr...

Embodiment 2

[0028] The use of functional small peptides extracted from deep-sea fish, as a protective agent or absorption-promoting agent for collagen, for cosmetics or health products or food or feed or medicine.

[0029] The most preferred method for extracting functional small peptides from deep-sea fish includes the following steps:

[0030]1) Step-by-step enzymatic hydrolysis: Add pure water homogenate to deep-sea fish surimi, then add Porphyra zebra protease and active short peptide for enzymatic hydrolysis, adjust the pH, add flavor protease for enzymatic hydrolysis, and obtain an enzymatic hydrolysis solution. 26 times the volume of water was added to deep-sea fish surimi, the amount of Porphyra zebra protease added was 6wt‰, the enzymatic hydrolysis temperature was 32°C, the pH was 8.0, the enzymatic hydrolysis time was 34min, and the enzyme was inactivated after the enzymatic hydrolysis. The amount of flavor protease added is 2wt%, the pH of enzymolysis is 6.4, the temperature o...

Embodiment 3

[0035] The use of functional small peptides extracted from deep-sea fish, as a protective agent or absorption-promoting agent for collagen, for cosmetics or health products or food or feed or medicine.

[0036] The most preferred method for extracting functional small peptides from deep-sea fish includes the following steps:

[0037] 1) Step-by-step enzymatic hydrolysis: Add pure water homogenate to deep-sea fish surimi, then add Porphyra zebra protease and active short peptide for enzymatic hydrolysis, adjust the pH, add flavor protease for enzymatic hydrolysis, and obtain an enzymatic hydrolysis solution. 30 times the volume of water was added to deep-sea fish surimi, the amount of Porphyra zebra protease added was 5.5wt‰, the enzymatic hydrolysis temperature was 32°C, the pH was 8.0, the enzymatic hydrolysis time was 34min, and the enzyme was inactivated after the enzymatic hydrolysis. The added amount of flavor protease is 1.8wt%, the pH of enzymolysis is 6.4, the temperat...

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Abstract

The invention discloses use of functional small peptides extracted from deep-sea fishes. The functional small peptides are applied to cosmetics or health-care products or food or feedstuff or drugs as collagen protectants or absorption promoters. According to the use of the functional small peptides extracted from the deep-sea fishes, the functional small peptides are prepared through the steps of step-by-step enzymolysis, isoelectric-point precipitation, secondary ultrafiltration and nanofiltration and freeze drying. The functional small peptides capable of protecting collagen from being decomposed by gastric acid can be obtained through carrying out synergistic enzymolysis by adopting marine protease and terrestrial protease, firstly, adopting porphyra yezoensis protease to carry out enzymolysis, and then, continuing to adopt flavorzyme to carry out enzymolysis. The use has the beneficial effects that the obtained functional small peptides and the collagen are bonded through intermolecular forces and hydrogen bonds, and the functional small peptides are attached to surfaces of collagen molecules and wrap the collagen molecules, so that the collagen can be protected from being decomposed by the gastric acid and can be promoted to reach connective tissue, cartilage and skin corium layer of animals.

Description

technical field [0001] The invention relates to the field of preparation of small peptides, in particular to the use of functional small peptides extracted from deep-sea fish. Background technique [0002] Due to the low content of naturally occurring marine active peptides in organisms and the difficulty of extraction, it is difficult to meet the needs of mass production and supply, and the cost of chemical synthetic peptides is expensive. Therefore, people pay more attention to the way of developing proteolysis products to obtain active polypeptides. Because the living environment of marine organisms is completely different from that of terrestrial organisms, such as extreme environments such as high pressure, low temperature, high temperature, and high salinity, in order to adapt to these extreme marine biological environments, marine biological proteins are closely related to terrestrial biological proteins in terms of amino acid composition and amino acid sequence. At ...

Claims

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Application Information

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IPC IPC(8): A61K38/39A61K47/42A61K47/64A61P17/00A61P17/02A61K8/64A61K8/65C12P21/06C07K14/46C07K1/36C07K1/34C07K1/30A61Q19/08A61Q19/02A23L33/18A23K20/147
CPCA61K38/39A23K20/147A23L33/18A23V2002/00A61K8/64A61K8/65A61K47/42A61Q19/02A61Q19/08C07K14/461C12P21/06A23V2200/30A23V2250/55
Inventor 周建波
Owner 兰溪市沉默生物科技有限公司
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