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Bacteria nutrient culture medium and preparation method thereof

A technology of culture medium preparation and bacterial nutrition, applied in biochemical equipment and methods, measuring devices, microbe determination/inspection, etc., to achieve efficient and rapid treatment, growth promotion, and low fat content

Inactive Publication Date: 2017-11-28
内蒙古东达獭兔循环产业研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the problems described above

Method used

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  • Bacteria nutrient culture medium and preparation method thereof
  • Bacteria nutrient culture medium and preparation method thereof
  • Bacteria nutrient culture medium and preparation method thereof

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preparation example Construction

[0059] The method for preparing bacterial nutrient medium of the present invention, bacterium is the pathogenic bacterium that causes rabbit to fall ill, and preparation method comprises the following steps:

[0060] Step 1, preparing rabbit meat powder;

[0061] Step 2: Take 0.6-1.0L of water and put it into a beaker, measure 25-35g of rabbit meat powder, 3-7g of NaCl, 1-3g of sodium citrate, 5-15g of glucose, 5-15g of lactose, and 5-15g of mannitol to dissolve Put in water and stir evenly to get a uniform mixed solution, then measure 15-25g of agar and add it to the mixed solution, heat the mixed solution at 90°C-98°C until the agar is completely melted, and keep stirring and mixing during this heating process liquid;

[0062] Step 3, adding 0.1-0.3 L of water to the mixed solution obtained in step 2 and adding agar, and then adding a pH regulator to adjust the pH value of the mixed solution to 6.8-7.5;

[0063] Step 4, sterilizing the mixed solution obtained in step 3 aft...

Embodiment 1

[0071] Step 101, preparing rabbit meat powder;

[0072] Step 1011, take 150g of raw rabbit meat, add 170ml of water and boil for 10 minutes, remove the water and grind into meat paste;

[0073] Step 1012, add protease to the meat paste obtained in step 1011 at an amount of 30,000 U / g and stir evenly, and place the uniformly stirred meat paste at 60° C. for 12 hours to obtain the heat-preserved meat paste. Add 400ml of water to the meat paste and boil for 15 minutes to inactivate the protease in the meat paste after heat preservation, and form rabbit meat slurry;

[0074] Step 1013, centrifuge the rabbit meat slurry obtained in step 1012 at a speed of 3000 rpm for 5 minutes in a centrifuge, filter out the residue and oil, obtain the filtered rabbit meat slurry, and process the filtered rabbit meat The slurry is dried to form rabbit meat powder.

[0075] Step 102, take 0.8L of water and put it into a beaker, take 30g of rabbit meat powder, 5g of NaCl, 2g of sodium citrate, 10g...

Embodiment 2

[0080] Step 101, preparing rabbit meat powder;

[0081] Step 1011, take 140g of raw rabbit meat, add 150ml of water and cook for 8 minutes, remove the water and grind into meat paste;

[0082]Step 1012, add protease to the meat paste obtained in step 1011 at an amount of 0.29 million U / g and stir evenly, and place the uniformly stirred meat paste at 65° C. for 11 hours to obtain the meat paste after heat preservation. Boil 300ml of meat paste in water for 12 minutes to inactivate the protease in the meat paste after heat preservation, and form rabbit meat slurry;

[0083] Step 1013, centrifuge the rabbit meat slurry obtained in step 1012 at a speed of 3200 rpm for 4 minutes in a centrifuge, filter out the residue and oil, obtain the filtered rabbit meat slurry, and process the filtered rabbit meat The slurry is dried to form rabbit meat powder.

[0084] Step 102, take 1.0L of water and put it into a beaker, take 35g of rabbit meat powder, 3g of NaCl, 3g of sodium citrate, 15...

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Abstract

The invention provides a method for preparing a nutrient culture medium for bacteria. The preparation process of the culture medium is scientific, including the preparation process of rabbit meat powder, and the prepared culture medium has a better effect on the cultivation of bacteria. The present invention also provides a bacterial nutrient medium, which can be used to cultivate common bacteria, and the medium can be used for drug susceptibility experiments, so as to achieve the effect of accurately and effectively using drugs to treat diseases caused by pathogenic bacteria .

Description

technical field [0001] The invention relates to the field of veterinary microorganisms, in particular to a bacterial nutrient medium and a preparation method thereof. Background technique [0002] At present, pathogenic bacteria in animal husbandry farms can cause livestock diseases, and it is difficult to accurately diagnose diseases caused by pathogenic bacteria through clinical symptoms, resulting in inaccurate use of drugs and drug abuse. For example, for rabbit farms, Pasteurella, Bordetella and Clostridium welchii are the main pathogenic bacteria in rex rabbits, but it is difficult to accurately diagnose the diseases caused by the above pathogenic bacteria through clinical symptoms, resulting in inappropriate medication, or Medication is symptomatic, but too much or too little dosage causes pathogenic bacteria to develop resistance to some commonly used antibiotics, resulting in symptomatic medication but no obvious effect. In order to effectively and quickly treat pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/045G01N2333/195
Inventor 王雷郅永伟李振海薛家兵
Owner 内蒙古东达獭兔循环产业研究院
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