Molding method for laying hen salpingitis

A technology of salpingitis and fallopian tubes, which is applied in the field of modeling hen salpingitis, can solve the problems of long-term inflammation, difficult operation, and high surgical environment requirements, and achieve obvious inflammatory characteristics, simple operation, and repeatability good sex effect

Active Publication Date: 2017-12-01
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. Due to the need for laparotomy, the operation is difficult, the requirements for the surgical environment are high, time-consuming and labor-intensive, the number of models made at the same time is limited, and it cannot be used for large-scale model-making in experimental research;
[0005] 2. The opening operation will cause great damage to laying hens, and it is easy to cause secondary systemic infection. After successful modeling, the physiological indicators and production performance of laying hens will change greatly, which does not conform to the actual situation of salpingitis in laying hens in actual production. Models to study the pathogenesis and the control effect of drugs are not suitable
Therefore, this kind of method is used to model salpingitis in laying hens, and often cannot produce typical salpingitis. Even if it is used in experimental animals such as rats and mice, it also has a long time to produce inflammation, mild symptoms, and difficult to control the dosage accurately. And vulnerable to microbial virulence and the impact of the body's immunity and other shortcomings

Method used

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  • Molding method for laying hen salpingitis
  • Molding method for laying hen salpingitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1, 10 Hai-Line gray laying hens in the laying period were randomly divided into 2 groups, 5 birds in each group, which were respectively the blank control group and the model-making group. The modeling group was made with 20% modeling preparation according to the aforementioned method, and the blank control group was not treated. Observe and record the mental state, egg production and abnormal egg production of laying hens in each group.

[0037] Results: The laying hens in the model-making group had clinical symptoms such as pain and restlessness, belly landing, feathers around the cloaca polluted by yellow-white sticky secretions, cloacal eversion and congestion and swelling, and eggs with blood, small eggs, The performance of abnormal eggs such as soft-shell eggs and sand-shell eggs. The laying hens in the blank control group behaved normally, without the above-mentioned performances.

Embodiment 2

[0038] Embodiment 2, according to embodiment one grouping and modeling. Three feathers were randomly selected from each group to be killed, and the abdominal cavity, ovaries and fallopian tubes were observed by laparotomy.

[0039] Results: There was a small amount of effusion in the peritoneal cavity of the laying hens in the model group, the oviduct was swollen and flushed, the diameter became thicker, and the wall of the oviduct became thicker after incision. The oviducts of laying hens in the blank control group were normal in appearance without the above-mentioned pathological changes.

Embodiment 3

[0040] Embodiment 3, according to embodiment 1 grouping and modeling. Three feathers were randomly selected from each group to be killed, and sections of oviduct and uterus were taken for observation.

[0041] Results: In the model group, the fallopian tube epithelial cells were exfoliated, degenerated and necrotic, and a large number of neutrophils infiltrated, and there was a tendency to diffuse. In the blank control group, the oviduct structure was clear without neutrophil infiltration, see figure 1 .

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Abstract

The invention relates to a molding method for laying hen salpingitis. The laying hen salpingitis molding is executed by a fallopian tube perfusion method. The molding method specifically comprises the following steps: according to a method of artificial insemination, keeping a handstand posture of a hen, evaginating and exposing the fallopian tube opening, injecting a molding preparation to the uterus part of the fallopian tube by using a hen seminiferous duct, wherein the dosage is 0.8-1.2 mL / hen, and keeping the handstand posture of the hen for 5-10 min. The molding method for the laying hen salpingitis has the advantages of good repeatability, remarkable inflammatory feature, and practical clinic, and is capable of providing an experiment method for the related research of the laying hen salpingitis.

Description

technical field [0001] The invention relates to a modeling method for experimental animal pathological models, in particular to a modeling method for laying hen salpingitis, which can provide experimental means for related research on laying hen salpingitis. Background technique [0002] Layer hen salpingitis is a relatively common disease of laying hens, which occurs mostly during the peak egg production period of laying hens. Clinically, it is characterized by hemorrhagic salpingitis, decreased egg production, and abnormal egg production, and often causes ovariitis and peritonitis, which brings great economic losses to the production of laying hens. The clinical manifestations of salpingitis are varied, but the main clinical symptoms are decreased egg production rate and abnormal eggs, such as soft-shelled eggs, small eggs, deformed eggs, thin-shelled eggs, sandy-shelled eggs, soft-shelled eggs and bloody eggs. eggs etc. The factors that cause this disease are very compl...

Claims

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Application Information

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IPC IPC(8): A61K31/05A61K31/7004A61K35/644A61K47/26A61K47/22A61P15/00
CPCA61K31/05A61K31/7004A61K35/644A61K47/22A61K47/26A61K2300/00
Inventor 张明军姬晓琪李兴安
Owner HUNAN AGRICULTURAL UNIV
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