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Endometrial stem cell preparation and application thereof

A stem cell preparation and endometrium technology, applied in the field of regenerative medicine and biology, can solve the problems of increasing the risk of secondary inflammation of the endometrium, increasing the risk of secondary infertility, abnormal growth of the endometrium, etc. Achieve the effect of avoiding damage to uterine tissue, avoiding immune rejection, and promoting repair

Inactive Publication Date: 2017-12-01
GUANGZHOU ZISHENG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, the number of patients with induced abortion and medical abortion has increased year by year. Multiple induced abortions in a short period of time can easily cause endometrial damage, resulting in abnormal growth of endometrium and intrauterine adhesions, increasing the risk of secondary inflammation of the endometrium. risk, making the risk of secondary infertility significantly increased
At present, the stem cell preparations used in the repair of scarred uterus mainly include autologous bone marrow stem cell preparations and autologous fat stem cell preparations. The source of stem cells needs to be sampled through wounds, which is not only difficult to sample, but also easy to cause certain physical damage to patients.

Method used

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  • Endometrial stem cell preparation and application thereof
  • Endometrial stem cell preparation and application thereof
  • Endometrial stem cell preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of endometrial stem cells

[0029] After consultation and consent, menstrual blood samples from healthy women were collected according to the instructions of the menstrual blood collection kit (Hangzhou Yiwensai, S-Evans biosciences); In liquid, store at 4°C, send the sample to the laboratory for centrifugation within 24h ~ 48h; then take the supernatant and quickly test whether there is bacterial contamination under sterile conditions, if it is negative, continue to isolate and identify, For the isolation, culture and identification process of endometrial stem cells, please refer to the following literature:

[0030] Zhou Hua, Song Lina, Zhang Min. Isolation, culture and identification of human endometrial stem cells in vitro. Chinese Journal of Traditional Chinese Medicine [J]. 2014.32(8):1907-1908.

Embodiment 2

[0031] Example 2 Preparation and Evaluation of Endometrial Stem Cell Preparation

[0032] 1. A preparation of endometrial stem cell preparation:

[0033] 1) Diluting human serum albumin with physiological saline to obtain a human serum albumin solution, wherein the volume percentage concentration of human serum albumin is 5%;

[0034] 2) Add HGF and SDF-1 respectively to the human serum albumin solution obtained in step 1), so that the final concentration of HGF is 20 ng / mL, and the final concentration of SDF-1 is 20 ng / mL;

[0035] 3) Using the human serum albumin solution containing HGF and SDF-1 obtained in step 2) to resuspend the endometrial stem cells obtained in Example 1 to obtain the endometrial stem cell preparation, wherein the concentration of endometrial stem cells is 5.0×10 6 a / mL;

[0036] 4) Divide the endometrial stem cell preparation obtained in step 3) into syringes, then put it into a sterile empty box, transfer the empty box containing the preparation t...

Embodiment 3

[0048] Example 3 Investigating the healing effect of different stem cell contents on the scarred uterus

[0049] Stem cell preparations were prepared according to the formula shown in Table 1, and then X-ray scoring was performed, and the results are shown in Table 2. Lane-Sandhu X-ray score showed: at 2, 4, and 8 weeks after operation, there was no statistical significance in group A compared with control group 1, P>0.05; the uterine scar repair effect of group B, group C combined with group D was significantly better In control group 1 and group A, the difference was statistically significant (P6 ~2.0×10 7 The effect of repairing uterine scar is more obvious when the concentration is 1 / mL.

[0050] Table 1

[0051] group

Specific composition

Control group 1

5% human serum albumin

Group A

2.5×10 6 cells / mL endometrial stem cells + 5% human serum albumin

Group B

5.0×10 6 cells / mL endometrial stem cells + 5% human serum albumi...

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Abstract

The invention belongs to the technical field of regenerative medicine and biology and particularly relates to an endometrial stem cell preparation and application thereof. The endometrial stem cell preparation includes endometrial stem cells, human serum albumin, hepatocyte growth factors and stromal cell-derived factors-1. The endometrial stem cells are selected as stem cell sources, so that material taking is convenient, the traumatic effect is avoided, and ethical limitation is avoided; and the endometrial stem cells and the cell factors take effects collaboratively, repairing of a scarred uterus is effectively facilitated, and the conversion rate of the stem cell preparation is increased. The invention further provides the application of the endometrial stem cell preparation in preparing of scarred uterus medicines.

Description

technical field [0001] The invention belongs to the technical field of regenerative medicine and biology, and in particular relates to an endometrial stem cell preparation and application thereof. Background technique [0002] In recent years, the number of patients with induced abortion and medical abortion has increased year by year. Multiple induced abortions in a short period of time can easily cause endometrial damage, resulting in abnormal growth of endometrium and intrauterine adhesions, increasing the risk of secondary inflammation of the endometrium. risk, making the risk of secondary infertility significantly increased. At present, the stem cell preparations used in the repair of scarred uterus mainly include autologous bone marrow stem cell preparations and autologous fat stem cell preparations. The source of stem cells needs to be sampled through wounds, which is not only difficult to sample, but also easy to cause certain physical damage to patients. Contents ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/38A61P15/08A61K38/18A61K38/19A61K35/545
CPCA61K38/385A61K35/545A61K38/1833A61K38/19A61K2300/00
Inventor 马颖马铎陈祥
Owner GUANGZHOU ZISHENG BIOLOGICAL TECH CO LTD
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