Porous artificial nerve stent and preparation method thereof

An artificial nerve and uniform technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve secondary injuries and other problems, and achieve the effect of promoting repair, high preparation efficiency, and simple preparation method

Active Publication Date: 2017-12-01
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the second stage, non-degradable materials such as polyethylene, polyvinyl chloride, etc. are used to artificially provide a microenvironment for nerve regeneration. After the repair is completed, a second operation is required to remove the material, which is likely to cause secondary damage.

Method used

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  • Porous artificial nerve stent and preparation method thereof
  • Porous artificial nerve stent and preparation method thereof
  • Porous artificial nerve stent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Using an electronic balance, weigh a certain amount of bacterial cellulose, configure 1L of 1% NaOH aqueous solution, heat it to 90°C with a magnetic stirrer, cut the bacterial cellulose into small pieces and immerse it in it, boil for 30 minutes, cut and disperse it with a mixer for about 5 minutes, Discard the supernatant after centrifuging at 1000rpm for 10min, wash with deionized water until neutral, add 60wt% sulfuric acid solution, perform hydrolysis in a water bath at 50°C in a constant temperature heating magnetic stirrer for 3h, add deionized water to the hydrolyzate After dilution, the diluted solution was centrifuged at 2000rpm for 10min, the supernatant was discarded, and an appropriate amount of NaOH solution was added to adjust the pH value to neutrality, then put into a dialysis bag, and dialyzed with ultrapure water for 3 days, during which time the water was changed every 4h. Freeze in a -80°C refrigerator for 1 hour, and then freeze-dry in a freeze drye...

Embodiment 2

[0035] Using an electronic balance, weigh a certain amount of bacterial cellulose, configure 1L of 1% NaOH aqueous solution, heat it to 90°C with a magnetic stirrer, cut the bacterial cellulose into small pieces and immerse it in it, boil for 30 minutes, cut and disperse it with a mixer for about 5 minutes, Discard the supernatant after centrifuging at 1000rpm for 10min, wash with deionized water until neutral, add 60wt% sulfuric acid solution, perform hydrolysis in a water bath at 50°C in a constant temperature heating magnetic stirrer for 2h, add deionized water to the hydrolyzate After dilution, the diluted solution was centrifuged at 2000rpm for 10min, the supernatant was discarded, and an appropriate amount of NaOH solution was added to adjust the pH value to neutrality, then put into a dialysis bag, and dialyzed with ultrapure water for 3 days, during which time the water was changed every 4h. Freeze in a -80°C refrigerator for 1 hour, and then freeze-dry in a freeze drye...

Embodiment 3

[0037] Using an electronic balance, weigh a certain amount of bacterial cellulose, configure 1L of 1% NaOH aqueous solution, heat it to 90°C with a magnetic stirrer, cut the bacterial cellulose into small pieces and immerse it in it, boil for 30 minutes, cut and disperse it with a mixer for about 5 minutes, Discard the supernatant after centrifuging at 1000rpm for 10min, wash with deionized water until neutral, add 60wt% sulfuric acid solution, perform hydrolysis in a water bath with a constant temperature heating magnetic stirrer at 50°C, add deionized water to the hydrolyzate for dilution After dilution, the solution was centrifuged at 2000rpm for 10min, the supernatant was discarded, and an appropriate amount of NaOH solution was added to adjust the pH value to neutrality, put into a dialysis bag, and dialyzed with ultrapure water for 3 days, during which the water was changed every 4h, - Freeze in a refrigerator at 80° C. for 1 hour, and then freeze-dry in a freeze dryer to...

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Abstract

The invention belongs to the technical field of the medical biological material, and particularly relates to a porous artificial nerve stent and a preparation method thereof. The lactic acid-glycolic acid copolymer / bacterial cellulose artificial nerve stent is prepared by using a freeze-drying method. The method comprises the following steps: firstly dissolving a certain amount of the bacterial cellulose crystal whisker and the lactic acid-glycolic acid copolymer in an organic solvent, and pouring in a tetrafluoroethylene mould, and freeze-drying to obtain the lactic acid-glycolic acid copolymer / bacterial cellulose artificial nerve stent. The prepared artificial nerve stent has the good biocompatibility, hydrophilic performance and higher porosity, and is capable of satisfying the requirement of the neural restoration. The bacterial cellulose is good for promoting the nerve cell adhesion and proliferation, and repairing the nerve injury. An autologous nerve graft method can be replaced by the artificial nerve stent, and the damaged nerves at two ends are bridged, so the nerve injury is repaired.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a porous artificial nerve support and a preparation method thereof. Background technique [0002] The problem of nerve damage has become more and more prominent in recent years, which has brought immeasurable losses and injuries to individuals and families. Nerve damage is mainly caused by accidents or sudden diseases. It can be repaired by autologous nerve transplantation. If the nerve damage is too serious and the damage length is too long, the source of autologous nerve transplantation is limited, and autologous nerve transplantation is likely to cause damage to other undamaged parts and affect other Therefore, it is particularly important to choose a method that can replace autologous transplantation to repair nerve damage. Artificial nerve scaffolds are increasingly attracting the attention of researchers because of their controllable microenvironmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/56A61L27/58
CPCA61L27/26A61L27/56A61L27/58A61L2430/32C08L67/04
Inventor 王欣宇林飞蔡正伟姚远
Owner WUHAN UNIV OF TECH
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