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A method for extracting cyclic adenosine monophosphate from microbial fermentation broth

A technology of microbial fermentation liquid and cyclic adenosine monophosphate, which is applied in the direction of sugar derivatives, sugar derivatives, sugar derivatives preparation, etc., can solve unreached problems and achieve low cost and simple operation

Active Publication Date: 2020-04-21
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method uses carbon chromatography to replace the traditional ion exchange and resin chromatography process, claiming that its yield can reach 75%, and the purity is not less than 97.5%, but this yield value has not been reached in practical applications

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  • A method for extracting cyclic adenosine monophosphate from microbial fermentation broth

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Embodiment 1

[0028] A method for extracting cyclic adenosine monophosphate from microbial fermentation broth, comprising the following steps:

[0029] 1) The microbial fermentation liquid is passed through a 0.45 μm filter membrane at a temperature of 65° C. to obtain a microbial fermentation filtrate;

[0030] 2) Use 2-6mm granular fruit shell activated carbon (purchased from Henan Gongyi City Zhonghuan Environmental Protection Material Trading Co., Ltd.) to fill the activated carbon column with a volume of 1:10 times, and use 2N NaOH solution at a temperature of 80 °C after packing the column (2.5 times the volume) soak the column for 2 hours, wash with water to neutral (pH value 7.0) to complete the activation, after activation, pickle with 2N HCl solution to pH value 2.5;

[0031] 3) Adjust the pH value of the microbial fermentation filtrate to 2.0 with 2N HCl solution, and put it on the column for adsorption to ensure that the pH value of the effluent is not greater than 2.0;

[0032...

Embodiment 2

[0035] A method for extracting cyclic adenosine monophosphate from microbial fermentation broth, comprising the following steps:

[0036] 1) The microbial fermentation liquid is passed through a 0.45 μm filter membrane at a temperature of 50° C. to obtain a microbial fermentation filtrate;

[0037] 2) Use 2-6mm granular fruit shell activated carbon to fill the activated carbon column with a volume of 1:10 times. After loading the column, soak the column with 2.5N NaOH solution (2 times the volume) at a temperature of 70°C for 1.5h, and wash it with water until Neutral (pH 7.0) completes the activation, after activation, it is acid-washed with 1.5N HCl solution to pH 2.0;

[0038] 3) Adjust the pH value of the microbial fermentation filtrate to 1.5 with 2N HCl solution, and put it on the column for adsorption to ensure that the pH value of the effluent is not greater than 1.5;

[0039] 4) Soak the column with alkaline solution for 1 hour before elution, and then use 5 times th...

Embodiment 3

[0042] A method for extracting cyclic adenosine monophosphate from microbial fermentation broth, comprising the following steps:

[0043] 1) The microbial fermentation liquid is passed through a 0.2 μm filter membrane at a temperature of 80° C. to obtain a microbial fermentation filtrate;

[0044]2) Use 2-6mm granular fruit shell activated carbon to fill the activated carbon column with a volume of 1:10 times. After loading the column, soak the column with 1.5N NaOH solution (3 times the volume) at a temperature of 80 ° C for 2.5 hours, and wash it with water until Neutral (pH value 7.0) completes the activation, after activation, pickle with 2.5N HCl solution to pH value 3.0;

[0045] 3) Adjust the pH value of the microbial fermentation filtrate to 2.2 with 2N HCl solution, and put it on the column for adsorption to ensure that the pH value of the effluent is not greater than 2.2;

[0046] 4) Soak the column with alkaline solution for 2 hours before elution, and then use 3 t...

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Abstract

The invention discloses a method for extracting cyclic adenosine monophosphate from microbial fermentation broth, and belongs to the technical field of microbial fermentation product extraction. In this method, the activated activated carbon column is acid-washed to a pH value of 2.0-3.0, and the microbial fermentation filtrate is adjusted to a pH value of 1.5-2.2. value, crystallization and drying to obtain cyclic adenosine monophosphate. The method has the advantages of simple operation, low cost, safety, high efficiency and no pollution, the yield of cyclic adenosine monophosphate is as high as 85%, and the purity is over 99%.

Description

technical field [0001] The invention relates to a method for extracting cyclic adenosine monophosphate from microbial fermentation broth, and belongs to the technical field of extraction of microbial fermentation products. Background technique [0002] Cyclic adenosine monophosphate is also known as adenosine-3',5'-cyclic phosphate, or cAMP for short. It is a substance that exists widely in the human body and has important physiological activities. In the human body, cyclic adenosine monophosphate can be generated from adenosine triphosphate through the catalysis of adenosine activating enzyme. As a second messenger substance involved in the regulation of cell functions, it plays a role in hormone regulation of physiological functions and substance metabolism in cells, and plays an important role in the metabolism of sugar and fat, as well as the synthesis and regulation of nucleic acids and proteins. [0003] Cyclic adenosine monophosphate is a protease activator, a deriv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/213C07H1/06
CPCC07H1/06C07H19/213
Inventor 常景玲李志刚张勉李东霄康书会
Owner HENAN INST OF SCI & TECH