Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for extracting and separating and purifying mannose oligosaccharides from yeast

A sugar oligosaccharide and yeast technology, applied in the field of extraction and separation and purification of mannose oligosaccharides, can solve the problems of separation and purification difficulties, limited promotion and application, and purification difficulties, and achieve the effect of improving production efficiency and reducing costs

Active Publication Date: 2020-11-20
FUDAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the many chemical synthesis steps of polysaccharides with specific structures, the purification is difficult, and the yield is usually only at the milligram level, which greatly limits its promotion and application; biological fermentation or natural product extraction can obtain higher yields, but due to the difficulty of separation and purification, It is difficult to obtain polysaccharides with high purity and precise structure
Among them, Manα1-2Man oligomannose and its derivatives are mostly limited to a small amount of laboratory synthesis due to their special structure, and their commercial products are still blank in the polysaccharide reagent market at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for extracting and separating and purifying mannose oligosaccharides from yeast
  • A method for extracting and separating and purifying mannose oligosaccharides from yeast
  • A method for extracting and separating and purifying mannose oligosaccharides from yeast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Accurately weigh 300 g of purified yeast and add it to a 2000 mL three-necked flask, put the flask in a vacuum drying oven at 50°C for 12 h, take out the flask from the vacuum drying oven, add 800 mL of acetic anhydride to it, and fix the flask in the oil Make sure that the liquid level of the reactant is immersed below the liquid level of the silicone oil in the oil bath, set the temperature of the oil bath to 90°C, add mechanical stirring, and set the stirring rate to 750 r / min. Slowly add 80 mL of concentrated sulfuric acid dropwise to the flask, and start timing when the temperature of the oil bath reaches and stabilizes at 90°C after the addition of concentrated sulfuric acid is completed, and stop heating when the reaction reaches 10 h. The flask was removed from the oil bath as a whole, and after cooling, the mixture in the flask was quickly poured into 5 L of deionized water and kept stirring vigorously, and allowed to stand still to allow the product to precipit...

Embodiment 2

[0039] Embodiment 2 adopts the same experimental procedure as in Example 1, and the difference is that the volume of the added vitriol oil is 60mL, the reaction time is 8 h, and the R of the product to be collected during the rough separation of the chromatographic column f The value is 0.3~0.4, and the product obtained after further purification is fully acetyl-protected mannotetraose, and 1~2 g of mannotetraose (Manα1-3Manα1-2Manα1-2Man) can be obtained after methanol / sodium methoxide deprotection.

[0040] Depend on Figure 1-4 It can be seen that through the separation and purification of the above steps, the purification of mannotriose and mannotetraose is basically achieved. The anomeric carbon of the polysaccharide is clearly visible in the figure 1 H and 13 C signal, due to the polysaccharide backbone in the hydrogen spectrum 1 The complex overlapping of the H signals is serious and cannot be assigned one by one. The signal assignments of the carbon spectrum are cle...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting, separating and purifying mannan oligosaccharides from yeast. The method specifically comprises the following steps of (1) using the yeast as a raw material, using acetic anhydride as a medium, and acidifying by concentrated sulfuric acid; (2) dissolving and extracting a crude product of acidifying by dichloromethane, so as to obtain an extracted crude product; (3) repeatedly reprecipitating and recrystallizing the extracted crude product, and refining; (4) performing column chromatography isolation and purifying, so as to obtain peracetylated protection type mannan oligosaccharides; (5) under the alkaline condition, performing deprotection on the peracetylated protection type mannan oligosaccharide, so as to obtain the mannan oligosaccharides, namely mannotriose (Man(alpha1)-2Man(alpha1)-2Man), and mannotetrose (Man(alpha1)-3Man(alpha1)-2Man(alpha1)-2Man). The method has the advantages that the method is simple, the preparation efficiency of the mannan oligosaccharides is effectively improved, and the cost is reduced.

Description

technical field [0001] The invention relates to the technical field of sugar separation and purification, in particular to a method for extracting, separating and purifying mannose oligosaccharides from yeast. Background technique [0002] Oligomannose is a common polysaccharide, which widely exists on the surface of various pathogenic microorganisms such as bacteria, fungi, and viruses in nature. Mannose-binding lectin (MBL), which is widely present in the immune system of organisms, can bind to mannose residues on the surface of such pathogenic microorganisms and play an important role in the innate immune response of organisms. [0003] Human immunodeficiency virus (Human Immunodeficiency Virus, HIV) has become one of the major threats to modern human health since its discovery. Studies in recent years have found that the surface of HIV capsid gp120 expresses a large number of oligomannose Mannose with a specific structure. 9 (GlcNAc) 2 , the structure is shown in form...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/06C07H1/06
CPCC07H1/06C07H3/06
Inventor 刘一江陈怀俊陈国颂
Owner FUDAN UNIV