Winged bean peptiglycan product and preparation method thereof
A quadrilateral bean and product technology, applied in the field of food processing, can solve the problems of serious loss of nutrient components, low added value of product development, restricting planting enthusiasm, etc., to improve lysine content, overcome special beany smell, and facilitate industrialization. production effect
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Embodiment 1
[0033] Take fresh square beans and soak them in an aqueous solution with a pH value of 6 for 10 hours. The amount of water added to submerge the square beans shall prevail, and the petals shall be broken and peeled. Add 100kg of peeled square beans to 700L of water for refining, keep stirring at 25°C for 60 minutes, filter once with three layers of gauze, and perform microfiltration once with a 100-mesh microfilter to obtain 740L of square bean filtrate I and 50kg of filter residue I; add 1600L of ethanol to filtrate I, let it stand for 25min, and centrifuge at 7000rpm for 24min to obtain 24.6kg of precipitate II and 2280L of supernatant II.
[0034] Add 20L water to dissolve the precipitate II, centrifuge at 8000rpm for 20min to obtain 23L supernatant III and 22.4kg precipitate III; add 80L water to suspend the precipitate III, then add trypsin to hydrolyze at 40°C for 4h, and the final concentration of trypsin reaches 400U / L or so to obtain enzymatic solution I; the supernat...
Embodiment 2
[0039] Take fresh square beans and soak them in an aqueous solution with a pH value of 6.5 for 12 hours. The amount of water added to submerge the square beans shall prevail, and the petals shall be broken and peeled. Add 150 kg of peeled square beans to 900 L of water for refining, keep stirring at 30 ° C for 60 minutes, filter once with three layers of gauze, and perform microfiltration once with a 150-mesh microfilter to obtain 980 L of square bean filtrate I and 60kg of filter residue I; add 2000L of ethanol to filtrate I, let it stand for 25min, and centrifuge at 8000rpm for 25min to obtain 38.5kg of precipitate II and 2850L of supernatant II.
[0040] Add 40L water to dissolve the precipitate II, centrifuge at 8000rpm for 20min to obtain 45L supernatant III and 34.5kg precipitate III; add 120L water to suspend the precipitate III, then add neutral protease and hydrolyze it at 45°C for 4.5h. When the concentration reached 450U / L, the enzymatic solution I was obtained; the...
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