Method for preparing dual-signal nitrogen bag graphene oxide quantum dot aptamer sensor

Abstract

The invention relates to a method for preparing a dual-signal nitrogen bag graphene oxide quantum dot aptamer sensor. The method comprises the steps that a prepared magnetic nanoparticle MCNCs / PMAA chelate ligand having high saturation susceptibility and biocompatibility serves as a capture probe, a gold-nitrogen bag graphene oxide quantum dot serves as Raman, a fluorescent substrate chelate aptamer short-chain complementary DNA serves as a signal molecule, and the aptamer, surface enhanced Raman spectroscopy and a fluorescence spectrum technology are utilized to construct the dual-signal sensor to implement toxin residue detection study. When toxins exist, the structure of the aptamer in a detection system is affected, and the toxins undergo specific recognition combination with the aptamer, thereby causing different concentrations of signal molecules to be released in the presence of different concentrations of toxins. Subsequently, the detection system is magnetically adsorbed. Free signal molecules released from supernatant can amplify Raman and fluorescence signals to different extents. According to characteristic peak maps of different Raman and fluorescence intensities, the purpose of dual-signal sensitive indirect detection of toxin residues is achieved.

Description

technical field [0001] The invention relates to a method for preparing a Raman fluorescent double-signal nitrogen-coated graphene oxide quantum dot aptamer sensor for toxin residue detection, specifically relates to the combination of sensor technology, Raman technology and fluorescence technology to prepare MCNCs / PMAA magnetic nano-microspheres chelate nucleic acid aptamers as capture molecules, gold-nitrogen-coated graphene oxide quantum dots simultaneously serve as Raman-enhanced substrates and fluorescent groups chelate aptamers and short-chain complementary DNA as signal molecules to achieve typical toxin residues Rapid, sensitive and effective detection of Raman and fluorescence dual signals. Background technique [0002] In the long river of history, there are countless food safety incidents caused by mycotoxins. At the same time, the occurrence of the incidents has also brought a baptism to the field of food safety testing technology again and again. Commonly used ...

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Problems solved by technology

[0003] At present, using MCNCs / PMAA magnetic nanospheres-aptamers as capture probes and cDNA-APS as signal molecules to construct a sensor system for sensitive and effective detection of antibiotic residues has not been reported.

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