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Probiotic compound with functions of enhancing immunity, regulating intestines and stomach and reducing weight and preparation method and application thereof

A technology for regulating the stomach and complexes, applied in the direction of bacteria used in food preparation, medical preparations containing active ingredients, applications, etc., can solve the problems of ineffective, toxic and side effects, single types of probiotic compositions, etc., to achieve Improving human immunity, weight loss effect, and anti-oxidation effect

Inactive Publication Date: 2017-12-15
鄂尔多斯一康医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved by the present invention is to overcome the single type of probiotic product effect and poor stability in the prior art, and the effect of the probiotic composition in the prior art is not obvious in reducing blood fat and promoting metabolism, and because it contains Chinese herbal medicine The ingredients inevitably have defects of toxic and side effects, so as to provide a probiotic compound with the functions of improving immunity, regulating the stomach and losing weight, its preparation method and application

Method used

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  • Probiotic compound with functions of enhancing immunity, regulating intestines and stomach and reducing weight and preparation method and application thereof
  • Probiotic compound with functions of enhancing immunity, regulating intestines and stomach and reducing weight and preparation method and application thereof
  • Probiotic compound with functions of enhancing immunity, regulating intestines and stomach and reducing weight and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0054] The preparation method of embodiment 1 probiotic compound

[0055] (1) Put the proportioned amount of compound culture medium into the fermenter, first add 4 / 5 of water, heat and stir to dissolve, then add water and the remaining water, and then sterilize at 121°C for 30 minutes for later use;

[0056] (2) Add the mother species of Lactobacillus acidophilus, Saccharomyces cerevisiae, or Bacillus licheniformis into sterilized physiological saline, use the inoculation tool to absorb the bacterial liquid, transplant it into the fermenter, seal it, and cultivate it at 30-40°C for 5 days;

[0057] (3) Add the second culture medium into the fermenter, measure the pH value again to be 3.0-4.0, and culture at 30-40° C. for 5-10 days.

[0058] The distribution ratio of each component of the compound medium is: 10 parts by weight of peptone, 5 parts by weight of sodium acetate, 1 part by volume of Tween 80, 3 parts by weight of yeast extract, 2 parts by weight of ammonium citrate...

Embodiment 2

[0063] Embodiment 2 The preparation method of probiotic compound

[0064] (1) Put the proportioned amount of compound culture medium into the fermenter, first add 4 / 5 of water, heat and stir to dissolve, then add water and the remaining water, and then sterilize at 121°C for 30 minutes for later use;

[0065] (2) Add the mother species of Lactobacillus acidophilus, Saccharomyces cerevisiae, or Bacillus licheniformis into sterilized physiological saline, use the inoculation tool to absorb the bacterial solution and transplant it into the fermenter, and then measure the pH value to 3.0-4.0, seal it, 30 Culture at -40°C for 5 days.

[0066] The distribution ratio of each component of the compound medium is: 10 parts by weight of peptone, 5 parts by weight of sodium acetate, 1 part by volume of Tween 80, 3 parts by weight of yeast extract, 2 parts by weight of ammonium citrate, 3000 parts by volume of water, and 10 parts by volume of soybean sprouts , 5 parts by weight of glucose...

Embodiment 3-6

[0070] The preparation method of embodiment 3-6 probiotic complex is the same as embodiment 1

[0071] Table 1 Raw material proportions by weight of each embodiment

[0072] The weight ratio of the raw materials of the probiotic compound is: 15-18 parts of Lactobacillus acidophilus, 2-6 parts of Saccharomyces cerevisiae, and 1.5-2.5 parts of Bacillus licheniformis.

[0073]

[0074]

[0075] Table 2 The weight ratio of the second medium raw materials in each embodiment

[0076] Example

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Abstract

The invention discloses a probiotic compound with functions of enhancing immunity, regulating intestines and stomach and reducing weight and a preparation method and an application thereof, belonging to the technical field of biological agents. The probiotic compound is prepared by common submerged fermentation of mother strain mycelia of lactobacillus acidophilus, saccharomyces cerevisiae, and bacillus licheniformis in a complex culture medium, wherein the complex culture medium comprises the following components: peptone, sodium acetate, Tween 80, yeast extract, ammonium citrate, soybean sprouts, glucose, soy peptone, beef broth, sodium chloride and water; and the lactobacillus acidophilus is Lactobacillus ACCC 19940, the saccharomyces cerevisiae is Saccharomyces ACCC 20107, and the bacillus licheniformis is Bacillus ACCC 01094. A probiotic component and a culture medium component in the probiotic compound provided by the invention are synergized and supplemented with each other, so that the probiotic compound has remarkable effects in improving immunity, regulating intestines and stomach and reducing weight.

Description

technical field [0001] The invention relates to the technical field of microbial preparations, in particular to a probiotic compound with the functions of improving immunity, regulating intestines and stomach, and reducing weight. Background technique [0002] Immunity is the body's own defense mechanism, which is the body's ability to recognize and eliminate any foreign matter (viruses, bacteria, etc.) . Under the action of the immune system of the normal body, the mutated cells will be inhibited and decomposed. However, for the immunocompromised population, since the immunocompetent cells cannot recognize the mutated cells, the mutated cells reproduce disorderly and eventually lead to disease. Therefore, improving the body's resistance to pathogens by enhancing the body's immunity has become a topic of increasing concern to the population today. [0003] Probiotics, also known as intestinal beneficial bacteria, refer to non-pathogenic microorganisms that can survive in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/064A61P1/14A61P37/04A61P3/04A23L33/135A61K35/742A61K35/747
CPCA23L33/135A61K35/742A61K35/747A61K36/064A23V2002/00A23V2400/113A61K2300/00A23V2200/32A23V2200/332A23V2200/324
Inventor 张永彬边志富郭瑞
Owner 鄂尔多斯一康医院
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