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Bacillus stearothermophilus spore culture aid, culture medium and application

A technology of thermophilic steatosis spores and culture medium, applied in the direction of bacteria, methods using spores, methods based on microorganisms, etc., can solve the problems of low efficiency and long culture period, so as to improve the germination rate, shorten the germination time, and improve the work efficiency. efficiency effect

Active Publication Date: 2020-07-31
CHINA NAT CENT FOR FOOD SAFETY RISK ASSESSMENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on this, it is necessary to provide a method for cultivating spores of Bacillus stearothermophilus for the problem that the efficiency of detection by Bacillus stearothermophilus is low due to the long cultivation period when bromocresol purple glucose peptone agar medium is used for spore culture. Adjuvants, media and applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Heat and mix 2g DL-tryptophan, 0.05g glutathione, 0.02g p-aminobenzoic acid and 4g xanthan gum, add 20g fructose, 2g beef powder, add water to 100mL, stir and dissolve to obtain a mixed solution , filter and sterilize the above mixed solution with a 0.22um sterile filter to prepare a culture aid.

[0040] Add 10.0g of peptone, 5.0g of glucose, and 4.0g of agar into 800mL of distilled water, heat and stir until completely dissolved, adjust the pH to 7.1±0.1, then add 0.6mL of 2% bromocresol purple ethanol solution, mix well, and set to volume to 990mL, autoclaved at 115°C for 30min, and naturally cooled to 60°C to prepare a culture medium.

[0041] Add 10 mL of culture aids to the culture medium cooled to 60°C and mix well to prepare a culture medium.

[0042] Add the spore suspension of Bacillus stearothermophilus to the medium to a final concentration of 5×10 spores 5 CFU, add 0.2mL of culture medium containing spore suspension to a sterile 5mL centrifuge tube to pre...

Embodiment 2

[0054] After heating and mixing 2g DL-tryptophan, 0.05g glutathione, 0.02g p-aminobenzoic acid, and 4g xanthan gum, add 20g fructose and 2g beef powder, add water to 100mL, stir and dissolve to obtain a mixed Liquid, the above mixed liquid is filtered and sterilized with a 0.22um sterile filter to prepare a culture aid.

[0055] Add 10.0g peptone and 5.0g glucose in 800mL distilled water, heat and stir until completely dissolved, adjust the pH to 7.1±0.1, then add 0.6mL of 2% bromocresol purple ethanol solution, mix well, and dilute to 990mL, 115 ℃ autoclave for 30 minutes, and naturally cooled to 60 ℃ to prepare the culture medium.

[0056] Add 10 mL of culture aids to the culture medium cooled to 60°C and mix well to prepare a culture medium. Use sterilized centrifuge tubes to pack 5mL / tube into caps to prepare detection culture tubes for use.

[0057] Add Bacillus stearothermophilus spores to the test culture tube until the final concentration of Bacillus stearothermophil...

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Abstract

The invention provides a culture additive for bacillus stearothermophilus spores. Every 100 mL of the culture additive contains 1-20 g of fructose, 0.1-10 g of DL-tryptophan, 0.01-5 g of glutathione and 0.1-10 mg of para-aminobenzoic acid. According to the culture additive for bacillus stearothermophilus spores, fructose, DL-tryptophan, glutathione and para-aminobenzoic acid contained in the culture additive can synergistically promote earlier germination of bacillus stearothermophilus spores, increase the germination rate, shorten the germination time of bacillus stearothermophilus spores and improve the working efficiency when bacillus stearothermophilus spores are used as standard strains.

Description

technical field [0001] The invention relates to the technical field of microbial culture medium, in particular to a bacillus stearothermophilus spore culture aid, culture medium and application. Background technique [0002] Bacillus stearothermophilus (Bacillus stearothermophilus) is a thermophilic aerobic bacillus, facultatively anaerobic, the bacterial propagule G-Lan's stain is purple, and the bacterial spores can be colored with malachite green. Its bacterial propagule has low requirements on medium, and it grows well on bromocresol purple glucose peptone agar, and its surface is rough and beige. The lowest growth temperature is 28°C, and the highest growth temperature is 70-77°C. The optimum temperature for growth and reproduction is 56-65°C, colonies can be formed after 24 hours of cultivation, and no colonies can be seen after 24 hours at 37°C. It grows well in the medium of pH 6.8-7.2, but cannot grow when the pH value is close to 5. [0003] The spores produced ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00C12N1/20C12Q1/04C12R1/07
CPCC12N1/20C12N3/00C12Q1/04
Inventor 吴永宁骆鹏杰陈霞李敬光赵云峰尚晓虹邱楠楠
Owner CHINA NAT CENT FOR FOOD SAFETY RISK ASSESSMENT